CAJ | 학술논문

采用水酶法结合挤压膨化预处理提取大豆蛋白,筛选5种蛋白酶,确定选用碱性蛋白酶作为水解酶;得出碱性蛋白酶提取大豆蛋白的最佳条件;加酶量109%、酶解温度50℃、酶解时间200min、料水比1:4.6、酶解pH8.5,经过验证与对比实验可知在最优酶解工艺条件下总蛋白提取率可达到93.76%左右,比传统的湿热预处理后酶解的总蛋白提取率78.83%提高了近15个百分点.
채용수매법결합제압팽화예처리제취대두단백,사선5충단백매,학정선용감성단백매작위수해매;득출감성단백매제취대두단백적최가조건;가매량109%、매해온도50℃、매해시간200min、료수비1:4.6、매해pH8.5,경과험증여대비실험가지재최우매해공예조건하총단백제취솔가체도93.76%좌우,비전통적습열예처리후매해적총단백제취솔78.83%제고료근15개백분점.
Enzymatic hydrolysis combined with pretreatment of extrusion was used to extract soybean protein. An alkaline proteinase has been screened from 5 proteinases. The optimal condition for soybean protein extraction by alkaline proteinase was 1.9% proteinase, reaction at 50 ℃, 3.3 h of hydrolysis, 1:4.6 of material and water ratio, and pH 8.5. The total protein extraction rate reached to 93.76% under this optimal condition, which was nearly 15% higher than total protein extraction using enzymatic hydrolysis combined with traditional treatment of heating.