CAJ | 학술논문

去垢剂是同时具有亲水极性基团和疏水非极性基团的双极性分子,能够使脂膜解体释放膜蛋白,并在溶液中为去膜状态下的膜蛋白提供疏水环境,维持和保护膜蛋白的疏水跨膜结构,在膜蛋白的结构和功能研究中有重要的意义.去垢剂的双极性和理化特性,如临界胶束浓度能够极大影响去垢剂和膜蛋白间的相互作用.在膜蛋白研究中,需要充分利用去垢剂的结构和特性:一方面,需要利用去垢剂代替脂质分子支持和稳定去膜状态下膜蛋白的结构和功能;另一方面,需要控制去垢荆和膜蛋白的相互作用,以满足膜蛋白结构研究如蛋白质结晶试验的要求.简要介绍了去垢荆在膜蛋白研究中的最新应用进展,涉及去垢剂在膜蛋白离体表达、分离和纯化、以及结构研究中的应用.
거구제시동시구유친수겁성기단화소수비겁성기단적쌍겁성분자,능구사지막해체석방막단백,병재용액중위거막상태하적막단백제공소수배경,유지화보호막단백적소수과막결구,재막단백적결구화공능연구중유중요적의의.거구제적쌍겁성화이화특성,여림계효속농도능구겁대영향거구제화막단백간적상호작용.재막단백연구중,수요충분이용거구제적결구화특성:일방면,수요이용거구제대체지질분자지지화은정거막상태하막단백적결구화공능;령일방면,수요공제거구형화막단백적상호작용,이만족막단백결구연구여단백질결정시험적요구.간요개소료거구형재막단백연구중적최신응용진전,섭급거구제재막단백리체표체、분리화순화、이급결구연구중적응용.
Detergents are amphiphilic molecules and able to solubilise membrane proteins from lipid membranes and provide hydrophobic protection for membrane proteins in hydrophilic solutions.Resultant detergent-solubilized membrane proteins can maintain their native trans-membrane structure in lipid-free environments.Therefore,detergents are valuable in structural and functional characterizations of membrane proteins.Interactions between detergents and membrane proteins are affected by the amphiphilic features together with the physical and the chemical properties of detergents,such as critical Micelle concentration.The research on membrane proteins requires investigators to make full use of these special characteristics of detergents.On the one hand,one can use detergents instead of lipid molecules to stabilize and maintain the structure and activity of lipid-free membrane proteins;on the other hand,one needs to control interactions between detergents and membrane proteins for structural investigations on membrane proteins using techniques such as protein crystallization.In this article,the latest bibliography were reviewed related to applications of detergents to in vitro expression,purification,and structural investigation of membrane proteins.