中国野生植物资源
中國野生植物資源
중국야생식물자원
Chinese Wild Plant Resources
2010年
1期
58-61
,共4页
何涛%淳泽%汪天杰%郑若劫
何濤%淳澤%汪天傑%鄭若劫
하도%순택%왕천걸%정약겁
腋芽%丛生芽%快速繁殖%增殖倍数%铁皮石斛
腋芽%叢生芽%快速繁殖%增殖倍數%鐵皮石斛
액아%총생아%쾌속번식%증식배수%철피석곡
axillary bud%multiple shoot%rapid propagation%propagation coefficient%Dendrobiumofficinale
目的 利用组织培养技术建立铁皮石斛的高效快繁体系,克隆繁殖种苗,为野生居群的恢复提供材料.方法 以铁皮石斛腋芽为外植体,研究不同浓度和配比的基本培养基、激素以及天然提取物对腋芽萌发生长,丛生芽的诱导增殖以及壮苗生根的影响,比较不同培养阶段的增殖倍数,筛选各时期的最适培养基.并对试管苗的形态学特征进行了调查.结果 1/2MS+NAA 0.2 mg/L+BA 1 mg/L适合腋芽的成活生长.BA与香蕉汁组合有利于丛生芽的诱导增殖,其最适比例为1/2MS+NAA 0.5 mg/L+BA2.0 ms/L+香蕉汁100 g/L,增殖倍数最大可达14.0,平均增殖倍数为6.4.培养基中添加100 g/L香蕉汁或200 g/L马铃薯汁均有利于壮苗生根.结论 铁皮石斛通过腋芽形成丛生芽途径建立高效的快繁技术体系获得优质种苗是可行的.
目的 利用組織培養技術建立鐵皮石斛的高效快繁體繫,剋隆繁殖種苗,為野生居群的恢複提供材料.方法 以鐵皮石斛腋芽為外植體,研究不同濃度和配比的基本培養基、激素以及天然提取物對腋芽萌髮生長,叢生芽的誘導增殖以及壯苗生根的影響,比較不同培養階段的增殖倍數,篩選各時期的最適培養基.併對試管苗的形態學特徵進行瞭調查.結果 1/2MS+NAA 0.2 mg/L+BA 1 mg/L適閤腋芽的成活生長.BA與香蕉汁組閤有利于叢生芽的誘導增殖,其最適比例為1/2MS+NAA 0.5 mg/L+BA2.0 ms/L+香蕉汁100 g/L,增殖倍數最大可達14.0,平均增殖倍數為6.4.培養基中添加100 g/L香蕉汁或200 g/L馬鈴藷汁均有利于壯苗生根.結論 鐵皮石斛通過腋芽形成叢生芽途徑建立高效的快繁技術體繫穫得優質種苗是可行的.
목적 이용조직배양기술건립철피석곡적고효쾌번체계,극륭번식충묘,위야생거군적회복제공재료.방법 이철피석곡액아위외식체,연구불동농도화배비적기본배양기、격소이급천연제취물대액아맹발생장,총생아적유도증식이급장묘생근적영향,비교불동배양계단적증식배수,사선각시기적최괄배양기.병대시관묘적형태학특정진행료조사.결과 1/2MS+NAA 0.2 mg/L+BA 1 mg/L괄합액아적성활생장.BA여향초즙조합유리우총생아적유도증식,기최괄비례위1/2MS+NAA 0.5 mg/L+BA2.0 ms/L+향초즙100 g/L,증식배수최대가체14.0,평균증식배수위6.4.배양기중첨가100 g/L향초즙혹200 g/L마령서즙균유리우장묘생근.결론 철피석곡통과액아형성총생아도경건립고효적쾌번기술체계획득우질충묘시가행적.
Objective A high efficiency rapid propagation system was established by tissue culture in order to conduct clone propagation of wild species and provide materials for resume of wild population.Methods Using the axillary buds of D. officinale as explant,effect of the differential concentration and ratio of basal medium,plant growth regulators and natural aqueous extracts were studied on growth of the axillary buds,multiple shoot proliferation and strength of plandets and rootsge.In addition,propagation coefficient and the optimal medium at different culture stage were compared and selected,and morphological characters of planters were investigated.Results 1/2MS medium added with NAA 0.2 mg/L and BA 1 mg/L WaS used for growth of the axillary buds.A combination of NAA 0.5 mg/L,BA 2.0 mg/Land banana juice 100 g/L was suggested to be the best medium for multiple shoot proliferation.The optimal culture medium for strong phndets and rootage was 1/2MS added NAA 0.5 mg/L and banana juice 100 g/L or tomato juice 200 g/L.Conclusion It is possible to obtain good quality plandets by the establishment of high efficiency rapid propagation system of multiple shoot.
