CAJ | 학술논문

目的:采用分子系统学方法分析铁皮石斛及其近缘种的遗传多样性,为准确进行基源鉴定、阐明属内的种问关系提供分子证据.方法:采集25种40个石斛样品分离提取DNA,PCR扩增ITS区及5.8S rDNA完整序列并测序,用Mega 3.1软件进行分子遗传进化分析.采用邻接法和最大简约法分析铁皮石斛亲缘关系构造系统树.结果:对25种石斛类植物的ITS区序列进行分析,两种方法得到的系统树基本一致.ITS序列在石斛种间存在极其显著的差异,铁皮石斛与其它各种石斛均有各自特异性的序列位点.结论:ITS序列作为石斛属植物种间的分子鉴定标记是可行的.
목적:채용분자계통학방법분석철피석곡급기근연충적유전다양성,위준학진행기원감정、천명속내적충문관계제공분자증거.방법:채집25충40개석곡양품분리제취DNA,PCR확증ITS구급5.8S rDNA완정서렬병측서,용Mega 3.1연건진행분자유전진화분석.채용린접법화최대간약법분석철피석곡친연관계구조계통수.결과:대25충석곡류식물적ITS구서렬진행분석,량충방법득도적계통수기본일치.ITS서렬재석곡충간존재겁기현저적차이,철피석곡여기타각충석곡균유각자특이성적서렬위점.결론:ITS서렬작위석곡속식물충간적분자감정표기시가행적.
AIM:To apply molecular systematic techniques to revealing the genetic diversity of medicinal plant of Dendrobium candidum and its related species in Dendrobium.METHODS:The internal transcribed spacer (ITS)as well as 5.8S rDNA sequences of 40 samples of 25 species of Dendrobium were carried out on 25 dendrobium samples and amplified using PCR method and sequenced.Mega 3.1 was used to analyze the genetic diversity within the genus.Phylogenetic relationships among species in Dendrobium were estimated by maximum parsimony and neighbor-joining methods to construct similar ITS trees. RESULTS:The phylogenetic analysis indicated that Dendrobium candidum had a markedly difference in the interspecies and possessed the characteristic sequence site.CONCLUSION:ITS sequences as a good molecular marker for authentication between Dendrobium and outgroup is feasibility.