中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2012年
9期
68-72
,共5页
程邦君%赵登秋%邬叶锋%汤建燕
程邦君%趙登鞦%鄔葉鋒%湯建燕
정방군%조등추%오협봉%탕건연
胆囊肿瘤%免疫组织化学%WWOX%Nrf2
膽囊腫瘤%免疫組織化學%WWOX%Nrf2
담낭종류%면역조직화학%WWOX%Nrf2
Gallbladder neoplasms%Immunohistochemistry%WWOX%Nrf2
[目的]研究WWOX抑癌基因和Nrf2转录因子在原发性胆囊癌(PGC)组织中的表达及其与临床病理的关系,以及WWOX和Nrf2表达的相关性.[方法]收集胆囊癌标本54例、胆囊腺瘤标本20例、慢性胆囊炎标本20例.应用免疫组织化学方法检测WWOX蛋白和Nrf2蛋白的表达情况,同时收集胆囊癌患者的临床病理资料,并将结果进行分析.[结果](1)WWOX蛋白在胆囊癌、胆囊腺瘤及慢性胆囊炎组织中阳性表达率分别为48.1％ (26/54)、75.0％ (15/20)和85.0％(17/20),差异有统计学意义(P＜0.01).WWOX蛋白阳性表达与病理学分级(P＜0.01)、Nevin分期(P＜0.05)、淋巴结转移(P＜0.01)有关.(2)Nrf2蛋白在胆囊癌、胆囊腺瘤及慢性胆囊炎组的阳性表达率分别为66.7％ (36/54)、20.0％ (4/20)及5.0％(1/20),差异有统计学意义(P＜0.01).Nrf2蛋白阳性表达水平与病理学分级(P＜0.05)、Nevin分期(P＜0.01)、淋巴结 转移(P＜0.01)有关.(3)WWOX阳性表达与Nrf2阳性表达均与患者的性别、年龄、病理类型及是否伴有结石无显著关系(P＞0.05).(4) WWOX阳性表达与Nrf2阳性表达呈负相关(r=-0.420,p＜0.01).[结论]WWOX和Nrf2表达可能是反映胆囊癌发生、进展、生物学行为和预后的重要生物学标记物；WWOX和Nrf2蛋白在胆囊癌中的表达呈明显的相关性,可能在胆囊癌的发生中起协同作用.
[目的]研究WWOX抑癌基因和Nrf2轉錄因子在原髮性膽囊癌(PGC)組織中的錶達及其與臨床病理的關繫,以及WWOX和Nrf2錶達的相關性.[方法]收集膽囊癌標本54例、膽囊腺瘤標本20例、慢性膽囊炎標本20例.應用免疫組織化學方法檢測WWOX蛋白和Nrf2蛋白的錶達情況,同時收集膽囊癌患者的臨床病理資料,併將結果進行分析.[結果](1)WWOX蛋白在膽囊癌、膽囊腺瘤及慢性膽囊炎組織中暘性錶達率分彆為48.1％ (26/54)、75.0％ (15/20)和85.0％(17/20),差異有統計學意義(P＜0.01).WWOX蛋白暘性錶達與病理學分級(P＜0.01)、Nevin分期(P＜0.05)、淋巴結轉移(P＜0.01)有關.(2)Nrf2蛋白在膽囊癌、膽囊腺瘤及慢性膽囊炎組的暘性錶達率分彆為66.7％ (36/54)、20.0％ (4/20)及5.0％(1/20),差異有統計學意義(P＜0.01).Nrf2蛋白暘性錶達水平與病理學分級(P＜0.05)、Nevin分期(P＜0.01)、淋巴結 轉移(P＜0.01)有關.(3)WWOX暘性錶達與Nrf2暘性錶達均與患者的性彆、年齡、病理類型及是否伴有結石無顯著關繫(P＞0.05).(4) WWOX暘性錶達與Nrf2暘性錶達呈負相關(r=-0.420,p＜0.01).[結論]WWOX和Nrf2錶達可能是反映膽囊癌髮生、進展、生物學行為和預後的重要生物學標記物；WWOX和Nrf2蛋白在膽囊癌中的錶達呈明顯的相關性,可能在膽囊癌的髮生中起協同作用.
[목적]연구WWOX억암기인화Nrf2전록인자재원발성담낭암(PGC)조직중적표체급기여림상병리적관계,이급WWOX화Nrf2표체적상관성.[방법]수집담낭암표본54례、담낭선류표본20례、만성담낭염표본20례.응용면역조직화학방법검측WWOX단백화Nrf2단백적표체정황,동시수집담낭암환자적림상병리자료,병장결과진행분석.[결과](1)WWOX단백재담낭암、담낭선류급만성담낭염조직중양성표체솔분별위48.1％ (26/54)、75.0％ (15/20)화85.0％(17/20),차이유통계학의의(P＜0.01).WWOX단백양성표체여병이학분급(P＜0.01)、Nevin분기(P＜0.05)、림파결전이(P＜0.01)유관.(2)Nrf2단백재담낭암、담낭선류급만성담낭염조적양성표체솔분별위66.7％ (36/54)、20.0％ (4/20)급5.0％(1/20),차이유통계학의의(P＜0.01).Nrf2단백양성표체수평여병이학분급(P＜0.05)、Nevin분기(P＜0.01)、림파결 전이(P＜0.01)유관.(3)WWOX양성표체여Nrf2양성표체균여환자적성별、년령、병리류형급시부반유결석무현저관계(P＞0.05).(4) WWOX양성표체여Nrf2양성표체정부상관(r=-0.420,p＜0.01).[결론]WWOX화Nrf2표체가능시반영담낭암발생、진전、생물학행위화예후적중요생물학표기물；WWOX화Nrf2단백재담낭암중적표체정명현적상관성,가능재담낭암적발생중기협동작용.
[Objective]To investigate expressions of WWOX tumor suppressive gene and Nrf2 transcription factor protein in gallbladder carcinoma and analyze its relation with clinical pathology,and its expression correlation.[Methods] Expressions of WWOX and Nrf2 were determined by an immunohistochemistrical method in 54 cases with carcinoma of gallbladder,20 with adenoma of gallbladder and 20 with chronic cholecystitis.The clinical pathology data from patients with carcinoma of gallbladder was analyzed.[Results] The positive rates of WWOX and Nrf2 protein in carcinoma of gallbladder,adenoma of gallbladder and chronic cholecystitis were 48.1％ (26/54) and 66.7％ (36/54),75.0％ (15/20) and 20.0％ (4/20),85.0％ (17/20) and 5.0％ ( 1/20),respectively.The positive expression of WWOX in carcinoma of gallbladder was significantly lower than those in adenoma of gallbladder and cholecystitis( P ＜0.05),however,the corresponding expression of Nrf2 protein was significantly higher than those in adenoma of gallbladder and cholecystitis ( P ＜ 0.05 ).Both expressions of WWOX and Nrf2 protein in gallbladder carcinoma had significant correlation with pathological grade( P ＜ 0.01 or P ＜ 0.05),Nevin staging(P ＜ 0.05 or P ＜0.01 ) and lymph node metastasis ( P ＜ 0.05 or P ＜ 0.01 ).Based on this findings,we could see the expression of WWOX in carcinoma of gallbladder was negative correlation with expression of Nrf2 protein ( r=- 0.420,P ＜0.01).[Conclusions]The expressive levels of WWOX and Nrf2 might be important biological markers for reflection the carcinogenesis,progression,biological behaviors ; Both expressions of WWOX and Nrf2 protein in gallbladder carcinoma have significant correlation,suggesting they may play a synergistic effect in the carcinogenesis of gallbladder carcinoma.
