CAJ | 학술논문

基因芯片通常指DNA芯片,其基本原理是将指大量寡核苷酸分子固定于支持物上,然后与标记的样品进行杂交,通过芯片扫描检测杂交信号的强弱进而判断样品中靶分子的数量.基因芯片能对微量样品中的核苷酸序列进行检测和分析,其高通量、快速、并行化采集生物信息的特点均优于其他传统基因检测技术,故已广泛应用于医学各领域研究.将近年来基因芯片技术在关节软骨生物学特性、形成及发育、损伤与修复、退变及再生等领域的应用进行综述.
기인심편통상지DNA심편,기기본원리시장지대량과핵감산분자고정우지지물상,연후여표기적양품진행잡교,통과심편소묘검측잡교신호적강약진이판단양품중파분자적수량.기인심편능대미량양품중적핵감산서렬진행검측화분석,기고통량、쾌속、병행화채집생물신식적특점균우우기타전통기인검측기술,고이엄범응용우의학각영역연구.장근년래기인심편기술재관절연골생물학특성、형성급발육、손상여수복、퇴변급재생등영역적응용진행종술.
DNA microarray generally refers to gene chips. Its basic principle is that a large number of oligonucleotide molecules were fixed on the support, and then hybridized with the labeled samples, and then the chip hybridization signal strength was scanned to determine the number of target in the samples. Gene chips can trace the nucleotide sequence in the samples for testing and analyzing. Its characteristics of high-throughput,rapid and parallel acquisition of biological information are better than that of other traditional gene detection technology. It has been widely used in various fields of medical research. This article reviews the application of DNA microarray technology in the study of orthopedics including biological characteristics, formation and development,injury and repair, degeneration and regeneration of articular cartilage.