CAJ | 학술논문

目的研究佛波酯对培养的人永生化角质形成细胞株HaCaT细胞环氧合酶-2(COX-2)表达水平的影响,探讨佛波酯的皮肤细胞毒性机制.方法以体外培养的HaCaT细胞为对象,采用RT-PCR和Western印迹方法 ,检测HaCaT细胞经0.1,1.0,10mg/L佛波酯处理24 h后COX-2mRNA和蛋白表达情况.结果对照组HaCaT细胞COX-2 mRNA和蛋白微弱表达或不表达,1.0,10.0 mg/L佛波酯组COX-2mRNA和蛋白表达水平均显著高于对照组和0.1 mg/L佛波酯组(P值均＜0.01),而且10.0 mg/L佛波酯组均高于1.0 mg/L佛波酯组,差异均有统计学意义(P＜0.01).结论佛波酯可能通过上调HaCaT细胞表达COX-2,促进前列腺素的合成释放,参与皮肤角质形成细胞恶性肿瘤的发生.
목적 연구불파지대배양적인영생화각질형성세포주HaCaT세포배양합매-2(COX-2)표체수평적영향,탐토불파지적피부세포독성궤제.방법 이체외배양적HaCaT세포위대상,채용RT-PCR화Western인적방법 ,검측HaCaT세포경0.1,1.0,10mg/L불파지처리24 h후COX-2mRNA화단백표체정황.결과 대조조HaCaT세포COX-2 mRNA화단백미약표체혹불표체,1.0,10.0 mg/L불파지조COX-2mRNA화단백표체수평균현저고우대조조화0.1 mg/L불파지조(P치균＜0.01),이차10.0 mg/L불파지조균고우1.0 mg/L불파지조,차이균유통계학의의(P＜0.01).결론 불파지가능통과상조HaCaT세포표체COX-2,촉진전렬선소적합성석방,삼여피부각질형성세포악성종류적발생.
Objective To investigate the effect of phorbol-12-myristate-13-acetate(PMA)on cyclooxygenase-2 (COX-2) mRNA and protein expression in cultured human HaCaT keratinocytes,and the mechanism for cytotoxity of PMA against keratinocytes.Methods RT-PCR and Westem blot were utilized to detect the expression of COX-2 mRNA and protein in cultured HaCaT ells at 24 hours after the treatment with various concentrations of PMA (0.1,1.0,10 mg/L).Results Without any treatment,there was no or a weak expression df COX-2 mRNA and protein in HaCaT cells;incubation witll PMA resulted in the induction of the expression of COX-2 in HaCaT cells.The expression levels of COX-2 mRNA and protein in 10 mg/L PMA-pretreated HaCaT cells were significantly higher than those in 1.0 mg/L PMA-pretreated HaCaT cells,which was in turn higher than that in 0.1 mg/L PMA-pretreated cells and untreated cells;the difrerence was statistically significant (all P＜0.01).Conclusion These results suggest that PMA may be involved in keratinocyte tumorigenesis by upregulating he expression of COX-2 as well as synthesis and release of prostaglandin in keratinocytes.