CAJ | 학술논문

目的探讨δ阿片受体激动剂(DADLE)对脓毒症大鼠血液动力学和氧代谢的影响.方法清洁级健康雄性SD大鼠80只,采用随机数字表法,将大鼠随机分为4组(n=20):假手术组(S组)仅穿线,不结扎穿孔;脓毒症组(SEP组)采用盲肠结扎穿孔法制备脓毒症模型;DADLE1组和DADLE2组分别于制模前0.5 h和制模后即刻经腹腔注射DADLE 10 mg/kg,浓度为0.5mg/ml.于制模后即刻、2 4 6 h(T1～4)时记录心排血量(CO)、MAP、左心室收缩压(LVSP)和心室压力最大变化速率(±dp/dtmax),计算心指数(CI).于T4时从左颈总动脉及右颈外静脉取血样,计算氧供给(DO2)、氧消耗(VO2)、氧摄取率(ER02).结果与S组比较,SEP组MAP、LVSP T2时升高,T3,4时降低,±dp/dtmax升高,DADLE1组和DADLE2组MAP T2时升高,T3,4时降低,LVSP T2,3时升高,T4时降低,±dP/dtmax升高,SEP组、DADLE1组和DADLE2组CI、DO2和VO2降低,ERO2升高.与SEP组比较,DADLE1组和DADLE2组MAP、LVSP、±dp/dtmax T3,4时升高,CI、DO2和VO2升高,ERO2降低(P＜0.05).DADLE1组和DADLE2组间上述各指标差异无统计学意义(P＞0.05).结论 DADLE能改善脓毒症大鼠血液动力学和氧代谢.
목적 탐토δ아편수체격동제(DADLE)대농독증대서혈액동역학화양대사적영향.방법청길급건강웅성SD대서80지,채용수궤수자표법,장대서수궤분위4조(n=20):가수술조(S조)부천선,불결찰천공;농독증조(SEP조)채용맹장결찰천공법제비농독증모형;DADLE1조화DADLE2조분별우제모전0.5 h화제모후즉각경복강주사DADLE 10 mg/kg,농도위0.5mg/ml.우제모후즉각、2 4 6 h(T1～4)시기록심배혈량(CO)、MAP、좌심실수축압(LVSP)화심실압력최대변화속솔(±dp/dtmax),계산심지수(CI).우T4시종좌경총동맥급우경외정맥취혈양,계산양공급(DO2)、양소모(VO2)、양섭취솔(ER02).결과 여S조비교,SEP조MAP、LVSP T2시승고,T3,4시강저,±dp/dtmax승고,DADLE1조화DADLE2조MAP T2시승고,T3,4시강저,LVSP T2,3시승고,T4시강저,±dP/dtmax승고,SEP조、DADLE1조화DADLE2조CI、DO2화VO2강저,ERO2승고.여SEP조비교,DADLE1조화DADLE2조MAP、LVSP、±dp/dtmax T3,4시승고,CI、DO2화VO2승고,ERO2강저(P＜0.05).DADLE1조화DADLE2조간상술각지표차이무통계학의의(P＞0.05).결론 DADLE능개선농독증대서혈액동역학화양대사.
Objective To investigate the effects of δ-opioid receptor agonist DADLE (D-Ala2-D-Leu5-enkephalin) on the hemodynamics and oxygen metabolism in rats with sepsis. Methods Eighty healthy male SD rats were randomly divided into 4 groups ( n = 20 each) : sham operation group (group S), sepsis group (group SEP) ,DADLE, group and DADLE2, group. Sepsis was induced by cecum ligation and puncture (CLP) in SEP, DADLE,and DADLE2 groups. In DADLE1 and DADLE2 groups, 0.5 mg/ml DADLE 10 mg/kg was injected intraperitoneally (IP) 0.5 h before CLP and immediately after CLP respectively. Mean arterial pressure (MAP), left ventricular systolic pressure (LVSP) and ± dp/dtmax were recorded at 0, 2, 4 and 6 h after CLP (T1-4). Blood samples from left common carotid artery and right external jugular vein were collected at T4 for blood gas analysis. The cardiac index (CI), O2 delivery (DO2), O2 consumption (VO2) and O2 extraction rate (ERO2) were calculated.Results Compared with group S, MAP and LVSP were significantly increased at T2, while decreased at T3,4, and ± dp/dtmax was significantly increased in group SEP, MAP was significantly increased at T2, while decreased at T3,4, LVSP was significantly increased at T2,3, while decreased at T4 , and ± dp/dtmax was significantly increased in DADLE, and DADLE2 groups, and CI, DO2 and VO2 were significantly decreased and ERO2 was increased in SEP, DADLE, and DADLE2 groups (P＜0.05). Compared with group SEP, MAP, LVSP and ± dp/dtmax at T3,4 and CI, DO2 and VO2 were significantly increased, while ERO2 was significantly decreased in DADLE1 and DADLE2 groups (P ＜ 0.05). There was no significant difference in the parameters mentioned above between DADLE1 and DADLE2 groups ( P ＞ 0.05). Conclusion δ-opioid receptor agonist DADLE can obviously improve the hemodynamics and oxygen metabolism in septic rats.