CAJ | 학술논문

目的探讨辛伐他汀对脓毒症大鼠内皮细胞功能的影响.方法清洁级雌性Wistar大鼠96只,体重200～250异,月龄4月.采用随机数字表法,将大鼠随机分为3组(n=32):假手术组(Ⅰ组)、脓毒症组(Ⅱ组)和辛伐他汀组(Ⅲ组).选模前,Ⅲ组经胃管给予辛伐他汀20 mg/kg,1次/d,连续2周,Ⅰ组和Ⅱ组用等容量生理盐水替代.Ⅱ组和Ⅲ组采用盲肠结扎穿孔法制备脓毒症模型.于术后3、6、24及48 h时分别取8只大鼠,采集颈动脉血样,测定血常规,计数WBC,用ELISA法检测血清B选择素浓度.结果与Ⅰ组比较,Ⅱ组和Ⅲ组WBC计数和血清B选择素浓度升高,术后6 h时达峰值(P＜0.05);与Ⅱ组比较,Ⅲ组WBC计数和血清E.选择素浓度降低(P＜0.05).结论辛伐他汀治疗脓毒症的作用机制与保护内皮细胞功能有关.
목적 탐토신벌타정대농독증대서내피세포공능적영향.방법청길급자성Wistar대서96지,체중200～250이,월령4월.채용수궤수자표법,장대서수궤분위3조(n=32):가수술조(Ⅰ조)、농독증조(Ⅱ조)화신벌타정조(Ⅲ조).선모전,Ⅲ조경위관급여신벌타정20 mg/kg,1차/d,련속2주,Ⅰ조화Ⅱ조용등용량생리염수체대.Ⅱ조화Ⅲ조채용맹장결찰천공법제비농독증모형.우술후3、6、24급48 h시분별취8지대서,채집경동맥혈양,측정혈상규,계수WBC,용ELISA법검측혈청B선택소농도.결과 여Ⅰ조비교,Ⅱ조화Ⅲ조WBC계수화혈청B선택소농도승고,술후6 h시체봉치(P＜0.05);여Ⅱ조비교,Ⅲ조WBC계수화혈청E.선택소농도강저(P＜0.05).결론 신벌타정치료농독증적작용궤제여보호내피세포공능유관.
Objective To investigate the effect of simvastatin on endothelial cell function in a rat model of sepsis. Methods Ninety-six pathogen-free female Wistar rats aged 4 months weighing 200-250 g were randomly divided into 3 groups (n = 32 each): group sham operation (group Ⅰ ); group sepsis (group Ⅱ )and group simvastatin + sepsis(group Ⅲ ) . Sepsis was induced by cecal ligation and puncture (CLP). In group Ⅲ simvastatin 20 mg/kg was given via a gastric tube once a day for 2 weeks. Blood samples were taken from carotid artery at 3,6, 24 and 48 h ( n = 8 at each time point) for WBC count and measurement of serum E-selectin concentration (by ELISA) . Results CLP significantly increased WBC count and serum E-selectin concentration in group Ⅱ as compared with group Ⅰ . The peak values were reached at 6 h after operation. Simvastatin pretreatment attenuated the sepsis-induced increase in WBC count and serum E-selectin concentration in group Ⅲ. Conclusion Protection of endothelial cell function is involved in the mechanism of treatment of sepsis with simvastatin.