CAJ | 학술논문

目的评价脊髓NF-κB信号通路在大鼠持续性术后痛中的作用.方法选择鞘内置管成功的成年雄性SD大鼠90只,体重200～250 g,采用随机数字表法,将其随机分为3组(n=30):假手术组(S组)、持续性术后痛组(SMIR组)和吡咯烷二硫代氨基甲酸盐组(PDTC组).SMIR组和PDTC组采用皮肤/肌肉切口牵拉法制备大鼠持续性术后痛模型；S组仅分离暴露肌肉,未牵拉.术后1d时开始,PDTC组于30s内鞘内注射NF-KB抑制剂PDTC 10 ng,S组和SMIR组鞘内注射等容量生理盐水,1次/d,连续7d.于术前1 d(T0)、术后1 d(T1)、3 d(T2)、7 d(T3)、12 d(T4)和22 d(T5)时,测定机械缩足反应阈值(MWT)；各时点MWT测定结束后,随机取5只大鼠,断头处死后取脊髓组织,采用ELISA法测定TNF-α含量.结果与S组比较,T1-5时SMIR组MWT降低,T2时PDTC组MWT降低,T3-5时SMIR组和PDTC组脊髓TNF-α含量升高(P＜0.05或0.01)；与SMIR组比较,T2-5时PDTC组MWT升高,T3.4时脊髓TNF-α含量降低(P＜0.05或0.01).结论脊髓NF-κB信号通路参与了大鼠持续性术后痛的发生发展.
목적 평개척수NF-κB신호통로재대서지속성술후통중적작용.방법 선택초내치관성공적성년웅성SD대서90지,체중200～250 g,채용수궤수자표법,장기수궤분위3조(n=30):가수술조(S조)、지속성술후통조(SMIR조)화필각완이류대안기갑산염조(PDTC조).SMIR조화PDTC조채용피부/기육절구견랍법제비대서지속성술후통모형；S조부분리폭로기육,미견랍.술후1d시개시,PDTC조우30s내초내주사NF-KB억제제PDTC 10 ng,S조화SMIR조초내주사등용량생리염수,1차/d,련속7d.우술전1 d(T0)、술후1 d(T1)、3 d(T2)、7 d(T3)、12 d(T4)화22 d(T5)시,측정궤계축족반응역치(MWT)；각시점MWT측정결속후,수궤취5지대서,단두처사후취척수조직,채용ELISA법측정TNF-α함량.결과 여S조비교,T1-5시SMIR조MWT강저,T2시PDTC조MWT강저,T3-5시SMIR조화PDTC조척수TNF-α함량승고(P＜0.05혹0.01)；여SMIR조비교,T2-5시PDTC조MWT승고,T3.4시척수TNF-α함량강저(P＜0.05혹0.01).결론 척수NF-κB신호통로삼여료대서지속성술후통적발생발전.
Objective To investigate the role of NF-κB signaling pathway in the spinal cord in persistent postoperative pain evoked by skin/muscle incision and retraction (SMIR) in rats.Methods Ninety male SD rats weighing 200-250 g in which intrathecal (IT) catheter was successfully implanted without complication were randomly divided into 3 groups ( n =30 each):group sham operation ( group S ) ; groups SMIR and group pyrrolidine dithiocarbarnate (a NF-κB inhibitor) (group PDTC).Persistent postoperative pain was evoked by SMIR according to the method described by Flatters in groups SMIR and PDTC.PDTC 10 ng in 10 μl was injected IT over 30 s once a day for 7 consecutive days after operation in group PDTC.Mechanical paw withdrawal threshold to von Frey filament stimulation (MWT) was measured at 1 d before (T0,baseline) and 1,3,7,12 and 22 d after surgery (T1-5).Five animals in each group were sacrificed at each time point after MWT measurement and their lumbar segments of the spinal cord were removed for determination of TNF-α content (by ELISA).Results SMIR significantly decreased MWT after operation at T1-5 and increased TNF-α content in the spinal cord at T3-5.PDTC significantly attenuated SMIR-induced hyperalgesia and increase in TNF-α content in the spinal cord.Conclusion NF-κB signaling pathway in the spinal cord plays an important role in the development of SMIR-induced persistent postoperafive pain in rats.