国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
14期
1057-1060
,共4页
急性肺损伤%油酸%肺表面活性物质%β肾上腺素能受体激动剂
急性肺損傷%油痠%肺錶麵活性物質%β腎上腺素能受體激動劑
급성폐손상%유산%폐표면활성물질%β신상선소능수체격동제
Acute lung injury%Oleic acid%Pulmonary surfactant%β-adrenergic receptor agonists
目的 探讨肺表面活性物质(PS)系统在油酸性急性肺损伤(ALI)时变化的作用机制及β肾上腺素能受体激动剂的干预作用,为临床治疗ALI开辟新的治疗措施提供理论依据和实践方向.方法 30只SD大鼠随机分成3组(每组10只),即油酸致ALI组(OA组)、异丙肾上腺素治疗组(ISO组)和生理盐水对照组(NS组).观察4 h后测定PaO2、PaCO2、pH值和血清肺表面活性蛋白A(SP-A)浓度;右肺灌洗后,测支气管肺泡灌洗液(BALF)中总蛋白(TP)、总磷脂(TPL)、磷脂酰胆碱(PC)和SP-A浓度;测左肺上段湿/干重比值(W/D),左肺中段作细胞膜β肾上腺素能受体的放射性配基结合分析,左肺下段行病理检查.结果 与NS组比较,OA组BALF中TPL、PC/TPL和SP-A明显减少(P值均＜0.01),而W/D、TP及血清SP-A升高(P＜0.05或P＜0.01).且OA组血清SP-A与同组BALF中SP-A呈负相关(r=-0.910,P＜0.05);与OA组比较,ISO组BALF中TPL、PC/TPL和SP-A升高,而W/D、TP及血清SP-A降低(P＜0.05或P＜0.01).结论 PS失活是油酸性ALI过程中的重要内在因素;ALI大鼠血清SP-A浓度显著升高,推测测定血清SP-A浓度可早期诊断ALI,β肾上腺素能受体激动剂在ALI早期具有一定的保护作用.
目的 探討肺錶麵活性物質(PS)繫統在油痠性急性肺損傷(ALI)時變化的作用機製及β腎上腺素能受體激動劑的榦預作用,為臨床治療ALI開闢新的治療措施提供理論依據和實踐方嚮.方法 30隻SD大鼠隨機分成3組(每組10隻),即油痠緻ALI組(OA組)、異丙腎上腺素治療組(ISO組)和生理鹽水對照組(NS組).觀察4 h後測定PaO2、PaCO2、pH值和血清肺錶麵活性蛋白A(SP-A)濃度;右肺灌洗後,測支氣管肺泡灌洗液(BALF)中總蛋白(TP)、總燐脂(TPL)、燐脂酰膽堿(PC)和SP-A濃度;測左肺上段濕/榦重比值(W/D),左肺中段作細胞膜β腎上腺素能受體的放射性配基結閤分析,左肺下段行病理檢查.結果 與NS組比較,OA組BALF中TPL、PC/TPL和SP-A明顯減少(P值均＜0.01),而W/D、TP及血清SP-A升高(P＜0.05或P＜0.01).且OA組血清SP-A與同組BALF中SP-A呈負相關(r=-0.910,P＜0.05);與OA組比較,ISO組BALF中TPL、PC/TPL和SP-A升高,而W/D、TP及血清SP-A降低(P＜0.05或P＜0.01).結論 PS失活是油痠性ALI過程中的重要內在因素;ALI大鼠血清SP-A濃度顯著升高,推測測定血清SP-A濃度可早期診斷ALI,β腎上腺素能受體激動劑在ALI早期具有一定的保護作用.
목적 탐토폐표면활성물질(PS)계통재유산성급성폐손상(ALI)시변화적작용궤제급β신상선소능수체격동제적간예작용,위림상치료ALI개벽신적치료조시제공이론의거화실천방향.방법 30지SD대서수궤분성3조(매조10지),즉유산치ALI조(OA조)、이병신상선소치료조(ISO조)화생리염수대조조(NS조).관찰4 h후측정PaO2、PaCO2、pH치화혈청폐표면활성단백A(SP-A)농도;우폐관세후,측지기관폐포관세액(BALF)중총단백(TP)、총린지(TPL)、린지선담감(PC)화SP-A농도;측좌폐상단습/간중비치(W/D),좌폐중단작세포막β신상선소능수체적방사성배기결합분석,좌폐하단행병리검사.결과 여NS조비교,OA조BALF중TPL、PC/TPL화SP-A명현감소(P치균＜0.01),이W/D、TP급혈청SP-A승고(P＜0.05혹P＜0.01).차OA조혈청SP-A여동조BALF중SP-A정부상관(r=-0.910,P＜0.05);여OA조비교,ISO조BALF중TPL、PC/TPL화SP-A승고,이W/D、TP급혈청SP-A강저(P＜0.05혹P＜0.01).결론 PS실활시유산성ALI과정중적중요내재인소;ALI대서혈청SP-A농도현저승고,추측측정혈청SP-A농도가조기진단ALI,β신상선소능수체격동제재ALI조기구유일정적보호작용.
Objective To investigate the changes of pulmonary surfactant (PS) in acute lung injury (ALI) induced by oleic acid and the intervention effect of β-adrenergic receptor agonists, and to provide a theoretical basis and practical direction for new therapeutic measures in the treatment of ALI. Methods Thirty Sprague-Dawley rats were randomly divided into ALI group induced by oleic acid (OA group, n = 10) , isoprenaline treatment group (ISO group, n =10) , and normal saline control group (NS group, n = 10). After four hours, PaO2 , PaCO2, pH, and the serum surfactant protein A (SP-A) levels were assayed. After right lung was lavaged, total proteins ( TP ), total phospholipids ( TPL), phosphatidylcholine (PC) and SP-A in the bronchoalveolar lavage fluid (BALF) were determined. The left lung was used for measurement of wet/dry weight ratio (W/D), beta-adrenergic receptor (β-AR) ,and histopathology. Results Compared with NS group, TPL, PC/TPL and SP-A in the BALF significantly decreased (all P ＜0. 01), W/D, TP and serum SP-A were increased ( P ＜0. 05 or P ＜0.01) in OA group. The values of SP-A in the BALF were negatively correlated to those in the serum in OA group ( r = - 0. 910, P ＜0. 05). Compared with OA group, TPL, PC/TPL and SP-A in the BALF increased, W/D, TP and serum SPA decreased in ISO group ( P ＜0. 05 or P ＜0. 01). Conclusions The PS inactivation may be an important internal cause during the course of ALI induced by oleic acid. The serum SP-A concentration is significantly elevated in ALI rats, so serum SP-A may be a marker for the early diagnosis of ALI, and β-adrenergic receptor agonists may play some protective role in the early stage of ALI.
