CAJ | 학술논문

目的分析乳腺良恶性钙化患者血清蛋白质表达谱,比较不同性质钙化的血清蛋白质差异.方法钙化型乳腺癌、良性钙化患者血清各10例,经双向凝胶电泳、基质辅助激光解析离子化-飞行时间质谱等对差异蛋白进行分析鉴定和验证.结果良恶性钙化组比较发现钙化型乳腺癌组血清中特异性的蛋白质2种,上调的蛋白质8种,下调的蛋白质7种.钙化型乳腺癌组触珠蛋白表达高于良性钙化组.结论乳腺的恶性钙化和良性钙化患者的血清中某些蛋白存在着差异表达,有可能作为鉴别乳腺钙化病变良恶性质的标志物分子.
목적 분석유선량악성개화환자혈청단백질표체보,비교불동성질개화적혈청단백질차이.방법 개화형유선암、량성개화환자혈청각10례,경쌍향응효전영、기질보조격광해석리자화-비행시간질보등대차이단백진행분석감정화험증.결과 량악성개화조비교발현개화형유선암조혈청중특이성적단백질2충,상조적단백질8충,하조적단백질7충.개화형유선암조촉주단백표체고우량성개화조.결론 유선적악성개화화량성개화환자적혈청중모사단백존재착차이표체,유가능작위감별유선개화병변량악성질적표지물분자.
Objective To find out serum protein difference between breast benign calcification and malignant calcification.Methods The serum samples of the patients with breast cancer and those with benign lesions were collected,10 samples in each category.They all had calcifications on mammygraphy.The mixed protein was assayed by using 2-DE method.The spots with marked different expression were excised from gels and digested into peptides.These peptides were analyzed by MALDI-TOF MS and the peptide mass fingerprintings were got.One of special protein was valided by Western blotting.Results In group of breast cancer with calcification,there were 2 specific proteins.There were 15 differential expression proteins,including 8 up-regulated,and 7 down-regulated.Conclusion Some identified serum proteins have different expression in patients with malignamt or benign calcifications,which may be helpful for the differential diagnosis of benign and malignant calcifications.