CAJ | 학술논문

水通道或水通道蛋白是水分运动的主要通道.以RD28 cDNA和RD28抗体为探针证明了蚕豆(Vicia fabaL.)保卫细胞中存在水通道蛋白,并以气孔运动为指标,结合抗体和抑制剂处理证明水通道蛋白是水分运动的主要通道.研究表明编码质膜水通道蛋白的RD28转录体在叶片保卫细胞、叶肉细胞和维管束中高表达,尤以保卫细胞中最多;荧光免疫染色和Confocal显微镜观察表明,RD28抗体反应主要位于保卫细胞质膜.进一步采用RD28抗体和水通道蛋白抑制剂--HgCl2 (25μmol/L)处理可抑制壳梭孢素(FC)、光照诱导的气孔开放和原生质体体积膨胀以及ABA诱导的气孔关闭,但这种抑制作用可以被水通道抑制剂的逆转剂β-巯基乙醇(ME)逆转.表明蚕豆保卫细胞中存在水通道蛋白并参与蚕豆保卫细胞的运动过程.
수통도혹수통도단백시수분운동적주요통도.이RD28 cDNA화RD28항체위탐침증명료잠두(Vicia fabaL.)보위세포중존재수통도단백,병이기공운동위지표,결합항체화억제제처리증명수통도단백시수분운동적주요통도.연구표명편마질막수통도단백적RD28전록체재협편보위세포、협육세포화유관속중고표체,우이보위세포중최다;형광면역염색화Confocal현미경관찰표명,RD28항체반응주요위우보위세포질막.진일보채용RD28항체화수통도단백억제제--HgCl2 (25μmol/L)처리가억제각사포소(FC)、광조유도적기공개방화원생질체체적팽창이급ABA유도적기공관폐,단저충억제작용가이피수통도억제제적역전제β-구기을순(ME)역전.표명잠두보위세포중존재수통도단백병삼여잠두보위세포적운동과정.
Water channels or aquaporins are the main pathways of water transport. Both the existence and function of aquaporins in the guard cells of Vicia faba L. Were investigated both by using RD28 cDNA and RD28 antibody as probes, and by controlling stomatal movement as a parameter combined with antibody and inhibitor of aquaporins respectively. The results revealed that RD28 mRNA, encoding a plasma membrane aquaporin, expressed in the mesophyll cells and vascular tissues of V． Faba, especially in guard cells. And the location of RD28-like proteins was mainly on plasma membrane of guard cells. The addition of 25　μmol/L HgCl2, an aquaporin blocker, and antibody of RD28 as well, greatly suppressed the stomatal opening or guard-cell protoplast swelling induced by fusicoccin and light, and closing induced by abscisic acid. However, 5 mmol/L β-mercaptoethanol, a reverse reagent of aquaporin blocker, reversed the inhibitory effect of HgCl2 pretreatment on stomatal opening (I.e., HgCl2 was removed after HgCl2 pretreatment for 10 min). The results suggest that the aquaporins in V. Faba are associated with stomatal movement.