CAJ | 학술논문

目的探讨尾静脉移植人脐血细胞对糖尿病模型小鼠血糖的影响.方法选择60只昆明小白鼠,随机抽取5只作为对照组,其余55只采用腹腔注射链脲佐菌素(STZ)的方法制备糖尿病鼠模型.成模后将小鼠随机分成移植组(26只)和糖尿病组(12只).将分离的人脐血单个核细胞于对数增长期用PKH26标记后,以2×106/ml经尾静脉注射入移植组小鼠体内.于注射后第4天和第14天观察3组小鼠的血糖和胰岛素水平,并用流式细胞仪和荧光显微镜观察PKH26标记的人脐血单个核细胞在体内的分布.结果人脐血单个核细胞植入第4天,移植组小鼠血糖和胰岛素水平与糖尿病组、对照组比较,差异均有统计学意义(P＜0.05);移植第14天移植组小鼠血糖水平及胰岛素水平与糖尿病组比较,差异均无统计学意义(P＞0.05).流式细胞检测发现移植第4天的糖尿病小鼠的胰、脾、肝、骨髓组织内均可见PKH26+细胞,而移植第14天上述各部位未发现PKH26+细胞,荧光显微镜观察上述部位的冷冻切片结果也支持这一结果.结论将人脐血细胞移植到未用免疫抑制剂的糖尿病小鼠体内可存活,并可以在一定时间内降低糖尿病小鼠的血糖水平.
목적 탐토미정맥이식인제혈세포대당뇨병모형소서혈당적영향.방법 선택60지곤명소백서,수궤추취5지작위대조조,기여55지채용복강주사련뇨좌균소(STZ)적방법제비당뇨병서모형.성모후장소서수궤분성이식조(26지)화당뇨병조(12지).장분리적인제혈단개핵세포우대수증장기용PKH26표기후,이2×106/ml경미정맥주사입이식조소서체내.우주사후제4천화제14천관찰3조소서적혈당화이도소수평,병용류식세포의화형광현미경관찰PKH26표기적인제혈단개핵세포재체내적분포.결과 인제혈단개핵세포식입제4천,이식조소서혈당화이도소수평여당뇨병조、대조조비교,차이균유통계학의의(P＜0.05);이식제14천이식조소서혈당수평급이도소수평여당뇨병조비교,차이균무통계학의의(P＞0.05).류식세포검측발현이식제4천적당뇨병소서적이、비、간、골수조직내균가견PKH26+세포,이이식제14천상술각부위미발현PKH26+세포,형광현미경관찰상술부위적냉동절편결과야지지저일결과.결론 장인제혈세포이식도미용면역억제제적당뇨병소서체내가존활,병가이재일정시간내강저당뇨병소서적혈당수평.
Objective To observe the therapeutic effect of human umbilical cord blood cells transplanted via caudal vein on blood glucose in diabetic mice.Methods Sixty Kunming mice were enrolled,5 of which were chosen as control group,the others given intraperitoneal injection of streptozotocin(STZ) to prepare diabetic mouse models.The prepared models were randomized into groups transportation (n=26) and diabetes (n=12).The isolated umbilical cord blood mononuclearcells were labeled by PKH26 in increased logarithmic phase,and then injected,in 2×106/ml,via caudal vein,into transportation group.The levels of serum glucose and insulin were determined on days 4,14 after injection,and the distribution of PHK26-labeled umbilical cord blood mononuclearcells were observed by flow cytometry and fluorescence microscopy.Results On day 4 after transportation,the serum glucose levels decreased significantly(P<0.05),but insulin levels increased when compared with pre-ransplantation(P<0.05).On day 14,serum glucose returned to pre-transplantation level,and insulin level decreased as compared with level on day 4(P<0.05),not significantly different from control.PKH26 positive cells were found in pancreases,spleen,liver and bone marrow on day 4 after transportation,but not on day 14.The frozen section in these parts supported these results by fluorescence microscopy.Conclusion Human umbilical cord blood cells,which can survive in diabetic mice without immunosuppressive agents ,can reduce blood glucose levels in a certain period of time.