CAJ | 학술논문

背景:目前研究肌肉与骨骼关系的常用动物模型均存在不足.已经有研究表明A型肉毒素可以使局部肌肉萎缩,而不影响周围其他的肌肉.目的:尝试是否可以用局部肌肉注射A型肉毒素的方法建立一种较为理想的肌肉萎缩的动物实验模型.方法:4月龄雄性Wistar大鼠25只,采用氯胺酮(0.2 mL/kg)和速眠新(0.2 mL/kg)肌注麻醉,在股骨背侧中部做-0.5 cm切口,暴露股四头肌,右侧股四头肌肌注2 U(0.2 mL)注射用A型肉毒毒素,左侧股四头肌注射等量生理盐水作为对照.分别于模型建立后的1,2,4,6和8周各取5只做肌肉大体、组织学观察.结果与结论:肌肉大体观察发现:与生理盐水侧对照组相比,注射A型肉毒素侧股四头肌体积明显缩小、质量明显降低(P＜0.01);肌肉组织学观察发现:注射A型肉毒素侧股四头肌较生理盐水侧明显萎缩、肌纤维变细、肌纤维细胞核聚集、肌纤维与肌纤维之间间距变小.肉毒素侧1周和2周出现肌肉质量突降,4,6,8周之间肌肉质量增加缓慢,而各时间点生理盐水侧肌肉质量呈显著增加的趋势.通过对局部肌肉注射A型肉毒素可以理想地建立单个目的肌肉萎缩的实验模型,此模型操作性强、重复性好、特异性高,可应用于肌肉与骨骼关系的相关研究.
배경:목전연구기육여골격관계적상용동물모형균존재불족.이경유연구표명A형육독소가이사국부기육위축,이불영향주위기타적기육.목적:상시시부가이용국부기육주사A형육독소적방법건립일충교위이상적기육위축적동물실험모형.방법:4월령웅성Wistar대서25지,채용록알동(0.2 mL/kg)화속면신(0.2 mL/kg)기주마취,재고골배측중부주-0.5 cm절구,폭로고사두기,우측고사두기기주2 U(0.2 mL)주사용A형육독독소,좌측고사두기주사등량생리염수작위대조.분별우모형건립후적1,2,4,6화8주각취5지주기육대체、조직학관찰.결과여결론:기육대체관찰발현:여생리염수측대조조상비,주사A형육독소측고사두기체적명현축소、질량명현강저(P＜0.01);기육조직학관찰발현:주사A형육독소측고사두기교생리염수측명현위축、기섬유변세、기섬유세포핵취집、기섬유여기섬유지간간거변소.육독소측1주화2주출현기육질량돌강,4,6,8주지간기육질량증가완만,이각시간점생리염수측기육질량정현저증가적추세.통과대국부기육주사A형육독소가이이상지건립단개목적기육위축적실험모형,차모형조작성강、중복성호、특이성고,가응용우기육여골격관계적상관연구.
BACKGROUND: There were disadvantages of animal models in the study of the relationship of muscle and skeleton. Recent study has been demonstrated the effect of Botulinum toxin type A to atrophy local muscle without influencing the surrounding muscle.OBJECTIVE: To construct a reasonable animal model of local muscle atrophy by local injection of Botulinum toxin type A.METHODS: Totally 25 male Wistar rats of 4 months were subjected to ketamine (0.2 mL/kg) and Sumianxin (0.2 mL/kg) by intramuscular injection. A 0.5-cm incision was made on the middle of dorsal femur to expose quadriceps femoris. Right quaddceps femods was injected with 2 Units (0.2 mL) of Botulinum toxin type A, and left quaddcaps femoris of the same rat with the same amount of saline as controls. At 1, 2, 4, 8, 8 weeks after injection, 5 rats were used to take gross observation and histological examination.RESULTS AND CONCLUSION: Gross observation of the muscle tissue showed that, compared with self-controlled group, the volume and weight of the quadriceps femods were decreased significantly (P < 0.01). The histological examination of muscle tissue showed the atrophy of the quadricaps femods from expedmental group was more obvious, the muscle fiber become thin,and the nuclei of the muscle fiber assemble together, with small distance betWeen muscle fibers. Weight of the quadricaps femods treated with Botulinum toxin type A was decreased at 1 and 2 weeks. The increase in weight of muscle was slow among muscle at 4, 6 and 8 weeks. The muscle weight showed an increased tendency in the saline side at vadous time points. Injecting Botulinum toxin type A into local muscle is a reasonable way to set up an experimental model of the atrophy of a destination muscle with strong practice, good repeatability, high stability, and may be used to examine the relationship of muscle and skeleton.