CAJ | 학술논문

目的:建立河南省不同采收期桔梗样品HPLC指纹图谱.方法:采用RP-HPLC法.选用Hypersil C_(18)(250 mm×4.6mm,5μm)色谱柱,流动相:乙腈-0.05%磷酸水溶液,梯度洗脱;流速:0.5 mL/min;柱温30℃;检测波长210 nm.结果:秋季样品整体相似度高于春季,以秋季采收为佳.结论:该法简便可行,重现性好,可为桔梗药材质量控制提供可靠的科学依据.
목적:건립하남성불동채수기길경양품HPLC지문도보.방법:채용RP-HPLC법.선용Hypersil C_(18)(250 mm×4.6mm,5μm)색보주,류동상:을정-0.05%린산수용액,제도세탈;류속:0.5 mL/min;주온30℃;검측파장210 nm.결과:추계양품정체상사도고우춘계,이추계채수위가.결론:해법간편가행,중현성호,가위길경약재질량공제제공가고적과학의거.
AIM:To establish HPLC fingerprints of Platycodon grandiflorum in different picking times in Henan province.METHODS:HPLC chromatography condition:Hypersil C_(18) column(250 mm×4.6 mm,5 μm);the mobile phase was acetonotrile with 0.05% phosphoric acid and the gradient elution mode was applied in chromatographic separation;The flow rate was 0.5 mL/min;The temperature of column was 30℃;the detective wavelength was set at 210 nm;RESULTS:The overall similarity in samples collected in autumn was higher than that in spring,and autumn was the appropriate for harvest.CONCLUSION:This method is simple and accurate with a good reproducibility.It provids a reliable scientific basis for the quality control of Platycodon grandiflorum.