中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2008年
8期
1307-1309,插1
,共4页
弥漫性脑损伤%疾病模型%动物
瀰漫性腦損傷%疾病模型%動物
미만성뇌손상%질병모형%동물
Diffuse brain injury%Disease models%animal
目的 复制大鼠弥漫性脑损伤模型,观察伤后病理生理变化.方法 参照Marmarou致伤装置进行改造,采用不同冲击力(80 cm、450 g,120 cm、450 g和180 cm、400 g)打击大鼠头部,制成闭合性弥漫性脑损伤动物模型,观察伤后动物神经体征、生命体征、感觉运动功能及脑组织病理形态学变化.结果 不同冲击力作用下,大鼠伤后神经系统体征、生命体征及感觉运动功能均明显改变.形态学检查证实,不同冲击力下大鼠脑组织均存在弥漫性脑损伤特征,而且随着冲击力增加,伤后病理生理变化更明显(均P＜0.05).结论 成功复制大鼠不同程度弥漫性脑损伤模型,在120 cm、450 g冲击力下动物伤后死亡率低且病理变化典型,适用于弥漫性脑损伤的相关研究.
目的 複製大鼠瀰漫性腦損傷模型,觀察傷後病理生理變化.方法 參照Marmarou緻傷裝置進行改造,採用不同遲擊力(80 cm、450 g,120 cm、450 g和180 cm、400 g)打擊大鼠頭部,製成閉閤性瀰漫性腦損傷動物模型,觀察傷後動物神經體徵、生命體徵、感覺運動功能及腦組織病理形態學變化.結果 不同遲擊力作用下,大鼠傷後神經繫統體徵、生命體徵及感覺運動功能均明顯改變.形態學檢查證實,不同遲擊力下大鼠腦組織均存在瀰漫性腦損傷特徵,而且隨著遲擊力增加,傷後病理生理變化更明顯(均P＜0.05).結論 成功複製大鼠不同程度瀰漫性腦損傷模型,在120 cm、450 g遲擊力下動物傷後死亡率低且病理變化典型,適用于瀰漫性腦損傷的相關研究.
목적 복제대서미만성뇌손상모형,관찰상후병리생리변화.방법 삼조Marmarou치상장치진행개조,채용불동충격력(80 cm、450 g,120 cm、450 g화180 cm、400 g)타격대서두부,제성폐합성미만성뇌손상동물모형,관찰상후동물신경체정、생명체정、감각운동공능급뇌조직병리형태학변화.결과 불동충격력작용하,대서상후신경계통체정、생명체정급감각운동공능균명현개변.형태학검사증실,불동충격력하대서뇌조직균존재미만성뇌손상특정,이차수착충격력증가,상후병리생리변화경명현(균P＜0.05).결론 성공복제대서불동정도미만성뇌손상모형,재120 cm、450 g충격력하동물상후사망솔저차병리변화전형,괄용우미만성뇌손상적상관연구.
Objective To develop-the rat model of diffuse brain injury(DBI)and to observe the pathophysio logical alteration of the rats after.traumatic injury.Methods A modified Marmarou's traumatic device W88 used to establish the animal model of diffuse brain injury with different degree through changing the impact condition that was applied on the rat brain(including 80cm 450g,120 cm 450g and 180cm 400g);the animal model was evaluated by observing the variations of nervous system signs,vital signs,sensorimotor function,and morphological changes of brain tissue in the rats that have received different impacts.Results The significant changes in nervous system signs,vital signs and sensorimotor function had been observed in all rats that were subjected to the different impact.The pathologic changcs of DBI in rats brain could he found in all groups.Moreover,this characteristic changes would have became more significant following the traumatic iaiury when the impact energy was increased.Conclusion The rat model of graded diffuse brain injury have been developed successfully.The moderate impact(120cm 450g)could produce low animal death rate and result in obvious pathologieal alterations in the injured rat brain.This impact condition would be suitable to be used in the study about DBI.
