中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2010年
10期
595-598,前插7
,共5页
刘红臻%钟敬泉%李景莎%苏国英%王静%张运
劉紅臻%鐘敬泉%李景莎%囌國英%王靜%張運
류홍진%종경천%리경사%소국영%왕정%장운
心室纤颤%缝隙连接蛋白Cx43%缝隙连接改造剂ZP123
心室纖顫%縫隙連接蛋白Cx43%縫隙連接改造劑ZP123
심실섬전%봉극련접단백Cx43%봉극련접개조제ZP123
Ventricular fibrillation%Connexin 43%Rotigaptide
目的 观察心室纤颤(室颤)发生后缝隙连接蛋白Cx43的表达以及缝隙连接改造剂ZP123对Cx43表达的影响.方法 按照随机数字表法将30只家猪分为假手术组、模型组和ZP123干预组,每组10只.以80 V电压持续刺激动物5 s诱发室颤;致颤前15 min ZP123组给予ZP123 1μg/kg静脉推注+ZP12310μg·kg-1·h-1微量泵泵入;模型组泵入生理盐水50 ml;假手术组动物不致颤也不补液.室颤持续8 min后开胸取左心室游离壁心肌,用免疫荧光结合激光共聚焦显微镜技术检测Cx43的分布及水平,用蛋白质免疫印迹法(Western blotting)定量检测Cx43蛋白表达.结果 假手术组Cx43荧光信号强,分布均匀;模型组Cx43荧光信号弱,呈不均一分布;ZP123干预组Cx43荧光信号增强,不均一分布减轻.与假手术组比较,模型组心室肌组织Cx43荧光信号面积百分比、积分吸光度(A)值及蛋白表达均明显下降[面积百分比:(0.64±0.36)%比(1.27±0.19)%,积分A值:15 201±2 613比30 634±4 975,Cx43蛋白表达:0.72±0.08比0.97±0.07,均P＜0.05];与模型组比较,ZP123干预组Cx43表达[面积百分比(0.96±0.16)%,积分A值22 100±4 404,Cx43蛋白表达0.82±0.04]均明显升高(均P＜0.05).结论 室颤发生时心肌组织Cx43表达减少;应用ZP123可减少或逆转Cx43的降解.
目的 觀察心室纖顫(室顫)髮生後縫隙連接蛋白Cx43的錶達以及縫隙連接改造劑ZP123對Cx43錶達的影響.方法 按照隨機數字錶法將30隻傢豬分為假手術組、模型組和ZP123榦預組,每組10隻.以80 V電壓持續刺激動物5 s誘髮室顫;緻顫前15 min ZP123組給予ZP123 1μg/kg靜脈推註+ZP12310μg·kg-1·h-1微量泵泵入;模型組泵入生理鹽水50 ml;假手術組動物不緻顫也不補液.室顫持續8 min後開胸取左心室遊離壁心肌,用免疫熒光結閤激光共聚焦顯微鏡技術檢測Cx43的分佈及水平,用蛋白質免疫印跡法(Western blotting)定量檢測Cx43蛋白錶達.結果 假手術組Cx43熒光信號彊,分佈均勻;模型組Cx43熒光信號弱,呈不均一分佈;ZP123榦預組Cx43熒光信號增彊,不均一分佈減輕.與假手術組比較,模型組心室肌組織Cx43熒光信號麵積百分比、積分吸光度(A)值及蛋白錶達均明顯下降[麵積百分比:(0.64±0.36)%比(1.27±0.19)%,積分A值:15 201±2 613比30 634±4 975,Cx43蛋白錶達:0.72±0.08比0.97±0.07,均P＜0.05];與模型組比較,ZP123榦預組Cx43錶達[麵積百分比(0.96±0.16)%,積分A值22 100±4 404,Cx43蛋白錶達0.82±0.04]均明顯升高(均P＜0.05).結論 室顫髮生時心肌組織Cx43錶達減少;應用ZP123可減少或逆轉Cx43的降解.
목적 관찰심실섬전(실전)발생후봉극련접단백Cx43적표체이급봉극련접개조제ZP123대Cx43표체적영향.방법 안조수궤수자표법장30지가저분위가수술조、모형조화ZP123간예조,매조10지.이80 V전압지속자격동물5 s유발실전;치전전15 min ZP123조급여ZP123 1μg/kg정맥추주+ZP12310μg·kg-1·h-1미량빙빙입;모형조빙입생리염수50 ml;가수술조동물불치전야불보액.실전지속8 min후개흉취좌심실유리벽심기,용면역형광결합격광공취초현미경기술검측Cx43적분포급수평,용단백질면역인적법(Western blotting)정량검측Cx43단백표체.결과 가수술조Cx43형광신호강,분포균균;모형조Cx43형광신호약,정불균일분포;ZP123간예조Cx43형광신호증강,불균일분포감경.여가수술조비교,모형조심실기조직Cx43형광신호면적백분비、적분흡광도(A)치급단백표체균명현하강[면적백분비:(0.64±0.36)%비(1.27±0.19)%,적분A치:15 201±2 613비30 634±4 975,Cx43단백표체:0.72±0.08비0.97±0.07,균P＜0.05];여모형조비교,ZP123간예조Cx43표체[면적백분비(0.96±0.16)%,적분A치22 100±4 404,Cx43단백표체0.82±0.04]균명현승고(균P＜0.05).결론 실전발생시심기조직Cx43표체감소;응용ZP123가감소혹역전Cx43적강해.
Objective To observe changes in connexin 43 (Cx43) after ventricular fibrillation (VF)and the effects of rotigaptide (ZP123) on Cx43. Methods Thirty domestic pigs were randomly assigned to three groups (10 in each group): sham group, model group and ZP123 group. VF was induced by an 80 V AC transthoracic shock for 5 seconds with the use of subcutaneous needles. Before the induction of VF,animals in ZP123 group were administered with ZP123 (1 μg/kg bolus+10 μg · kg-1 · h-1 dissolved in 50 ml normal saline and pumped for 15 minutes). Those in model group received 50 ml normal saline pumped for 15 minutes. For pigs in sham group VF was not induced and no fluid was given. After 8 minutes of VF,animals were euthanized and myocardial tissues were harvested along the long axis of each left ventricular free wall. Immunofluorescence combined with laser scanning confocal microscope was used to detect the distribution of Cx43. Western blotting was used for quantitative determination of Cx43 protein expression.Results Immunofluorescence signals for Cx43 in sham group were strong and regularly distributed. In model group, Cx43 signals were weak and distributed in heterogeneity, while in ZP123 group, Cx43 signals were enhanced and their distribution were much more orderly. Compared with sham group, the percentage area and the optical densities (A value) of Cx43 fluorescence signals and Cx43 protein expression were significantly decreased in model group [the percentage area: (0. 64 ± 0. 36) % vs. (1.27 ± 0. 19) %, A value:15 201±2 613 vs. 30 634±4 975, Cx43 protein expression: 0. 72±0. 08 vs. 0. 97±0. 07, all P＜0. 05]. The level of Cx43 expression in ZP 123 group [the percentage area (0. 96±0. 16)%, A value 22 100±4 404,Cx43 protein expression 0. 82 ±0.04] was much higher than model group (all P＜0.05). Conclusion During VF, down-regulation of myocardial Cx43 expression occurred, which could be attenuated by administration of ZP123.
