CAJ | 학술논문

目的通过检测在不同肿瘤细胞株和不同病变前列腺组织中的前列腺特异膜抗原(prostate specific membrane antigen,PSMA)及其变异体5(PSMA5) DNA水平,进一步了解和阐明PSMA及PSMA5的生物学特性.方法通过建立的荧光定量逆转录聚合酶链反应(fluorescent quantitation reverse transcriptase polymerase chain reaction,FQ-RT-PCR)检测肿瘤细胞株及不同病变前列腺组织中的PSMA及PSMA5 DNA.结果前列腺癌组织和前列腺增生组织中的PSMA 及PSMA5 DNA 含量均明显高于正常前列腺组织(F=3.40、11.94,均P＜0.05),且在前列腺癌组织中PSMA5 DNA含量明显高于PSMA DNA(P＜0.01).结论 PSMA及PSMA5在不同肿瘤细胞株和前列腺组织中的差异表达,提示了作为前列腺癌的肿瘤标志物PSMA5更具有肿瘤特异性.
목적 통과검측재불동종류세포주화불동병변전렬선조직중적전렬선특이막항원(prostate specific membrane antigen,PSMA)급기변이체5(PSMA5) DNA수평,진일보료해화천명PSMA급PSMA5적생물학특성.방법 통과건립적형광정량역전록취합매련반응(fluorescent quantitation reverse transcriptase polymerase chain reaction,FQ-RT-PCR)검측종류세포주급불동병변전렬선조직중적PSMA급PSMA5 DNA.결과 전렬선암조직화전렬선증생조직중적PSMA 급PSMA5 DNA 함량균명현고우정상전렬선조직(F=3.40、11.94,균P＜0.05),차재전렬선암조직중PSMA5 DNA함량명현고우PSMA DNA(P＜0.01).결론 PSMA급PSMA5재불동종류세포주화전렬선조직중적차이표체,제시료작위전렬선암적종류표지물PSMA5경구유종류특이성.
Objective To understand and illuminate the bionomic characteristics of prostate specific membrane antigen (PSMA) and splicing variant PSMA5, through detecting the DNA levels of them in different tumor cell strains and prostate tissues. Methods The fluorescent quantization reverse transcriptase PCR (FQ-RT-PCR) method built up by our research group was used to detect the PSMA and variant PSMA5 DNA levels in different tumor cell strains and prostate tissues. Results The PSMA and PSMA5 DNA levels in tumor cell strains and pathological prostatic tissues were obviously more than those of the normal prostatic tissues (F=3.40, 11.94, both P＜0.05), and the PSMA5 DNA level was much higher than was the PSMA DNA level in prostatic carcinoma tissues (P＜0.05). Conclusions The different expressions between PSMA and PSMA5 in different tumor cells and prostatic tissues show that PSMA5 is more specific than PSMA as a prostate carcinoma tumor marker.