中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2010年
11期
678-683
,共6页
倪伟嘉%李玉成%吴家媛%倪龙兴
倪偉嘉%李玉成%吳傢媛%倪龍興
예위가%리옥성%오가원%예룡흥
牙乳头%微孔过滤器%细胞分化%牙本质再生
牙乳頭%微孔過濾器%細胞分化%牙本質再生
아유두%미공과려기%세포분화%아본질재생
Dental papilla%Micropore filters%Cell differentiation%Dentin regeneration
目的 观察SD大鼠牙乳头细胞在复合有转化生长因子β1(transforming growth factor-β1,TGF-β1)的微孔滤膜上形成牙本质样结构的能力,为组织工程化具有一定规则形状的牙本质提供实验依据.方法 取传代培养的第二代SD大鼠牙乳头细胞消化、离心,所得细胞团与复合有TGF-β1的微孔滤膜相结合,体外培养6 d或体内移植2周,观察附合物上细胞生长和硬组织形成情况.结果 体外培养观察到靠近微孔滤膜的牙乳头细胞发生极化,呈高柱状沿滤膜排列,并有细胞突起伸人材料的多孔结构中,牙本质涎蛋白(dentin sialoprotein,DSP)与牙本质基质蛋白1(dentin matrix protein-1,DMP-1)表达阳性;体内移植2周移植物可见管状基质沿滤膜表面沉积,扫描电镜可见厚度基本一致的管状牙本质样结构,DSP、DMP-1在高柱状细胞、管状基质和邻近的牙乳头细胞中均有表达.结论 复合有TGF-β1的微孔滤膜能够有效趋化和诱导成牙本质细胞前体细胞在其表面分化,并均匀分泌基质,矿化形成结构规则的牙本质样结构.
目的 觀察SD大鼠牙乳頭細胞在複閤有轉化生長因子β1(transforming growth factor-β1,TGF-β1)的微孔濾膜上形成牙本質樣結構的能力,為組織工程化具有一定規則形狀的牙本質提供實驗依據.方法 取傳代培養的第二代SD大鼠牙乳頭細胞消化、離心,所得細胞糰與複閤有TGF-β1的微孔濾膜相結閤,體外培養6 d或體內移植2週,觀察附閤物上細胞生長和硬組織形成情況.結果 體外培養觀察到靠近微孔濾膜的牙乳頭細胞髮生極化,呈高柱狀沿濾膜排列,併有細胞突起伸人材料的多孔結構中,牙本質涎蛋白(dentin sialoprotein,DSP)與牙本質基質蛋白1(dentin matrix protein-1,DMP-1)錶達暘性;體內移植2週移植物可見管狀基質沿濾膜錶麵沉積,掃描電鏡可見厚度基本一緻的管狀牙本質樣結構,DSP、DMP-1在高柱狀細胞、管狀基質和鄰近的牙乳頭細胞中均有錶達.結論 複閤有TGF-β1的微孔濾膜能夠有效趨化和誘導成牙本質細胞前體細胞在其錶麵分化,併均勻分泌基質,礦化形成結構規則的牙本質樣結構.
목적 관찰SD대서아유두세포재복합유전화생장인자β1(transforming growth factor-β1,TGF-β1)적미공려막상형성아본질양결구적능력,위조직공정화구유일정규칙형상적아본질제공실험의거.방법 취전대배양적제이대SD대서아유두세포소화、리심,소득세포단여복합유TGF-β1적미공려막상결합,체외배양6 d혹체내이식2주,관찰부합물상세포생장화경조직형성정황.결과 체외배양관찰도고근미공려막적아유두세포발생겁화,정고주상연려막배렬,병유세포돌기신인재료적다공결구중,아본질연단백(dentin sialoprotein,DSP)여아본질기질단백1(dentin matrix protein-1,DMP-1)표체양성;체내이식2주이식물가견관상기질연려막표면침적,소묘전경가견후도기본일치적관상아본질양결구,DSP、DMP-1재고주상세포、관상기질화린근적아유두세포중균유표체.결론 복합유TGF-β1적미공려막능구유효추화화유도성아본질세포전체세포재기표면분화,병균균분비기질,광화형성결구규칙적아본질양결구.
Objective To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β1 (TGF-β1). Methods The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass.The cell mass was seeded on the millipore filter combined with TGF-β1. The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed. Results A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein(DSP) and dentin matrix protein-1 ( DMP-1 ) were positive in these cells.After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter. Conclusions The millipore filter combined with TGF-β1 could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.
