中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
1期
30-32
,共3页
郭启仓%赵长全%邬鹏宇%张宇%陈玲%陈晨%蒲国华%张春青
郭啟倉%趙長全%鄔鵬宇%張宇%陳玲%陳晨%蒲國華%張春青
곽계창%조장전%오붕우%장우%진령%진신%포국화%장춘청
热休克蛋白%去甲肾上腺素%供心保护%超氧化物岐化酶%丙二醛
熱休剋蛋白%去甲腎上腺素%供心保護%超氧化物岐化酶%丙二醛
열휴극단백%거갑신상선소%공심보호%초양화물기화매%병이철
HSP70%Norepinephrine%Donor heart myocardial protection%SOD%MDA
目的 研究去甲肾上腺素预处理诱导内源性热休克蛋白70( HSP70)表达规律,探讨内源性HSP70对离体供心的保护作用,以寻求更为有效的延长供心保存的方法.方法 将60只SD大鼠按随机数字法分为实验组(腹腔注射去甲肾上腺素)和对照组(腹腔注射生理盐水),均对SD大鼠行腹腔注射,每24小时给药一次,共给药6次.2组均于最后1次给药24h后采用免疫组织化学法分别于供心离体后5个时间点(0、4、6、8、10 h)观察左心室前壁心肌组织的HSP70表达情况以及超氧化物歧化酶(SOD)和丙二醛(MDA)的含量.结果 随着时间的延长,2组HSP70表达逐渐下降,与对照组比较,实验组同时间点的HSP70表达量明显增高,组间差异有统计学意义(P＜0.01).实验组的SOD活性明显高于各时间点相对应的对照组；但随着供心保存时间的延长,活性逐渐下降；实验组保存10 h后明显下降,而对照组保存6h下降明显.各时间点实验组心肌组织中MDA的含量明显低于对照组,组间差异有统计学意义[(0.35±0.09)μmol/g比(1.98±0.55) μmol/g,(0.35±0.09)μmol/g比(2.13±0.57) μmol/g,(0.52±0.10) μmol/g比(2.94±0.55)μmol/g,(1.28±0.24) μmol/g比(4.02 ±0.97) μmol/g,(2.70±0.41) μmol/g比(5.31±1.34)mol/g,P＜0.01].实验组SOD比对照组同时间点的差异有统计学意义[(250.87±61.23) U/mg比(146.50±40.37) U/mg,(246.36 ±57.42) U/mg比(143.28±39.21) U/mg,( 235.85±54.33) U/mg比(118.46±35.81) U/mg,(205.61±43.26) U/mg比(79.37±21.65) U/mg,( 112.03±31.75) U/mg比(50.26±14.98) U/mg,P＜0.01].结论 去甲肾上腺素预处理诱导心肌HSP70高表达对供心具有明显的保护效应,其MDA含量降低、SOD活性升高,增强心肌抗氧自由基损伤能力,从而发挥其对供心心肌细胞保护作用.
目的 研究去甲腎上腺素預處理誘導內源性熱休剋蛋白70( HSP70)錶達規律,探討內源性HSP70對離體供心的保護作用,以尋求更為有效的延長供心保存的方法.方法 將60隻SD大鼠按隨機數字法分為實驗組(腹腔註射去甲腎上腺素)和對照組(腹腔註射生理鹽水),均對SD大鼠行腹腔註射,每24小時給藥一次,共給藥6次.2組均于最後1次給藥24h後採用免疫組織化學法分彆于供心離體後5箇時間點(0、4、6、8、10 h)觀察左心室前壁心肌組織的HSP70錶達情況以及超氧化物歧化酶(SOD)和丙二醛(MDA)的含量.結果 隨著時間的延長,2組HSP70錶達逐漸下降,與對照組比較,實驗組同時間點的HSP70錶達量明顯增高,組間差異有統計學意義(P＜0.01).實驗組的SOD活性明顯高于各時間點相對應的對照組；但隨著供心保存時間的延長,活性逐漸下降；實驗組保存10 h後明顯下降,而對照組保存6h下降明顯.各時間點實驗組心肌組織中MDA的含量明顯低于對照組,組間差異有統計學意義[(0.35±0.09)μmol/g比(1.98±0.55) μmol/g,(0.35±0.09)μmol/g比(2.13±0.57) μmol/g,(0.52±0.10) μmol/g比(2.94±0.55)μmol/g,(1.28±0.24) μmol/g比(4.02 ±0.97) μmol/g,(2.70±0.41) μmol/g比(5.31±1.34)mol/g,P＜0.01].實驗組SOD比對照組同時間點的差異有統計學意義[(250.87±61.23) U/mg比(146.50±40.37) U/mg,(246.36 ±57.42) U/mg比(143.28±39.21) U/mg,( 235.85±54.33) U/mg比(118.46±35.81) U/mg,(205.61±43.26) U/mg比(79.37±21.65) U/mg,( 112.03±31.75) U/mg比(50.26±14.98) U/mg,P＜0.01].結論 去甲腎上腺素預處理誘導心肌HSP70高錶達對供心具有明顯的保護效應,其MDA含量降低、SOD活性升高,增彊心肌抗氧自由基損傷能力,從而髮揮其對供心心肌細胞保護作用.
목적 연구거갑신상선소예처리유도내원성열휴극단백70( HSP70)표체규률,탐토내원성HSP70대리체공심적보호작용,이심구경위유효적연장공심보존적방법.방법 장60지SD대서안수궤수자법분위실험조(복강주사거갑신상선소)화대조조(복강주사생리염수),균대SD대서행복강주사,매24소시급약일차,공급약6차.2조균우최후1차급약24h후채용면역조직화학법분별우공심리체후5개시간점(0、4、6、8、10 h)관찰좌심실전벽심기조직적HSP70표체정황이급초양화물기화매(SOD)화병이철(MDA)적함량.결과 수착시간적연장,2조HSP70표체축점하강,여대조조비교,실험조동시간점적HSP70표체량명현증고,조간차이유통계학의의(P＜0.01).실험조적SOD활성명현고우각시간점상대응적대조조；단수착공심보존시간적연장,활성축점하강；실험조보존10 h후명현하강,이대조조보존6h하강명현.각시간점실험조심기조직중MDA적함량명현저우대조조,조간차이유통계학의의[(0.35±0.09)μmol/g비(1.98±0.55) μmol/g,(0.35±0.09)μmol/g비(2.13±0.57) μmol/g,(0.52±0.10) μmol/g비(2.94±0.55)μmol/g,(1.28±0.24) μmol/g비(4.02 ±0.97) μmol/g,(2.70±0.41) μmol/g비(5.31±1.34)mol/g,P＜0.01].실험조SOD비대조조동시간점적차이유통계학의의[(250.87±61.23) U/mg비(146.50±40.37) U/mg,(246.36 ±57.42) U/mg비(143.28±39.21) U/mg,( 235.85±54.33) U/mg비(118.46±35.81) U/mg,(205.61±43.26) U/mg비(79.37±21.65) U/mg,( 112.03±31.75) U/mg비(50.26±14.98) U/mg,P＜0.01].결론 거갑신상선소예처리유도심기HSP70고표체대공심구유명현적보호효응,기MDA함량강저、SOD활성승고,증강심기항양자유기손상능력,종이발휘기대공심심기세포보호작용.
Objective To investigate protective effect of endogenous Heat Shock Protein(HSP) 70 in vitro for donor heart,and to find a more effective method to extend the donor heart preservation by pretreatment of Norepinephrine.Methods Sixty rats were divided into two groups randomly( thirty in each group).Experimental group:SD rats were injected Norepinephrine by introperitoneal once every 24 hours,which were administered 6 times.Control group: SD rats were injected normal saline by introperitoneal once every 24 hours,which were administered 6 times.SD rats in each group were killed for collecting myocardium tissue after twenty-four hours from the last injection.The expression of myocardial HSP70,superoxide dismutase(SOD) activity and malondialdehyde(MDA) contents were checked by immunohistochemistry.Results Compared with the control group,the expression of HSP70 was higher in experimental group at the five time points [ ( 9.36 ± 2.53 ) ％ vs ( 3.59 ± 1.01 ) ％,( 9.09 ± 2.34) ％ vs (3.24±0.87)％,(8.65 ±2.11)％ vs(2.08 ±0.56)％,(6.99 ± 1.77)％ vs(1.06 ±0.26)％,(2.80 ±0.65 )％ vs(0.25 ±0.05 )％,P ＜0.01 ].In the experimental group,the SOD activity in the myocardial tissue decreased significantly after preservation of ten hours,which equaled to the six-hours in the control group.Compared with the control group,the contents of MDA in the experimental group had a lower expression at the five time points,which showed a continuous increasing tendency following the preserved time.In the experimental group,the MDA contents in the myocardial tissue after being preserved ten hours had a significantly increase at the five time points [ (0.349 ± 0.092 ) nmol/mg vs ( 1.98 ± 0.55 ) nmol/mg,( 0.349 ± 0.092 ) nmol/mg vs ( 2.13 ± 0.57 ) nmol/mg,(0.525 ± 0.102 ) nmol/mg vs ( 2.94 ± 0.55 ) nmol/mg,( 1.283 ± 0.241 ) nmol/mg vs ( 4.02 ± 0.97 ) nmol/mg,(2.70 ± 0.415 ) nmol/mg vs ( 5.31 ± 1.34) nmol/ mg,P ＜ 0.01 ].The activity of SOD in the experimental group had a higher expression at the five time points[ (250.87 ± 61.23) U/mg vs( 146.50 ± 40.37) U/mg,(246.36 ± 57.42) U/mg vs ( 143.28 ± 39.21 ) U/mg,(235.85 ± 54.33 ) U/mg vs ( 118.46 ± 35.81 ) U/mg,(205.61 ± 43.26 ) U/mgvs (79.37 ± 21.65 ) U/mg,( 112.03 ± 31.75 ) U/mg vs ( 50.26 ± 14.98 ) U/mg,P ＜ 0.01 ],which showed a continuous decreasing tendency following the preserved time.Conclusions The HSP70 expression in myocardium is high with Norepinephrine preconditioning,which is a beneficial protection for donor heart by decreased contents of MDA,increased SOD activity and increased myocardial anti-oxidation.
