中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2011年
9期
637-640
,共4页
孙爱丽%倪一虹%关广聚%邓浩萍%刘元涛%陈诗鸿%孙福敦%庄向华%胡晓燕%邓景惕
孫愛麗%倪一虹%關廣聚%鄧浩萍%劉元濤%陳詩鴻%孫福敦%莊嚮華%鬍曉燕%鄧景惕
손애려%예일홍%관엄취%산호평%류원도%진시홍%손복돈%장향화%호효연%산경척
糖尿病肾病%白蛋白尿%尿胞外体%Th1细胞%Th2细胞
糖尿病腎病%白蛋白尿%尿胞外體%Th1細胞%Th2細胞
당뇨병신병%백단백뇨%뇨포외체%Th1세포%Th2세포
Diabetic nephropathy%Albuminuria%Urinary exosomes%Th1 cells%Th2 cells
目的 探讨2型糖尿病(DM)患者尿胞外体1型辅助性T细胞/2型辅助性T细胞(Th1/Th2)的变化与2型糖尿病肾病(DN)发生发展的相关性.方法 选取120例2型糖尿病患者及健康对照组30例为对象,根据尿白蛋白肌酐比(UACR),2型糖尿病患者分为糖尿病非肾病组(DM,40例,UACR＜30 mg/gCr)、微量白蛋白尿组(DN1,50例,UACR≥30 ～300mg/gCr)和临床白蛋白尿组(DN2,30例,UACR＞300 mg/gCr).用特异性单克隆抗体提纯尿胞外体.用酶联免疫吸附法( ELISA)检测尿胞外体干扰素γ(IFN-γ)和白细胞介素4(IL-4)水平.用多元逐步回归方法分析尿胞外体IFN-γ/IL-4比值与糖化血红蛋白(HbA1c)、胆固醇(CH)、UACR、血肌酐(Scr)、尿素氮(BUN)相关性.结果 DM、DN1、DN2组胞外体Th1/Th2水平显著高于健康对照组(0.8089 ±0.2458、0.8993±0.3515、0.8571±0.2470比0.6198±0.1769,均P＜0.01).DN1组胞外体Th 1/Th2显著高于DM组(P＜0.01).尿胞外体IFN-γ/IL-4与UACR(r=0.213,P=0.015)、BUN( r=0.292,P=0.001)呈正相关.逐步多元回归分析显示,BUN是尿胞外体IFN-γ/IL-4的独立影响因素(β=0.246,P=0.006).结论 尿胞外体Th1/Th2漂移与2型糖尿病肾病密切相关,可能在糖尿病早期肾病发病过程中起重要的作用.
目的 探討2型糖尿病(DM)患者尿胞外體1型輔助性T細胞/2型輔助性T細胞(Th1/Th2)的變化與2型糖尿病腎病(DN)髮生髮展的相關性.方法 選取120例2型糖尿病患者及健康對照組30例為對象,根據尿白蛋白肌酐比(UACR),2型糖尿病患者分為糖尿病非腎病組(DM,40例,UACR＜30 mg/gCr)、微量白蛋白尿組(DN1,50例,UACR≥30 ～300mg/gCr)和臨床白蛋白尿組(DN2,30例,UACR＞300 mg/gCr).用特異性單剋隆抗體提純尿胞外體.用酶聯免疫吸附法( ELISA)檢測尿胞外體榦擾素γ(IFN-γ)和白細胞介素4(IL-4)水平.用多元逐步迴歸方法分析尿胞外體IFN-γ/IL-4比值與糖化血紅蛋白(HbA1c)、膽固醇(CH)、UACR、血肌酐(Scr)、尿素氮(BUN)相關性.結果 DM、DN1、DN2組胞外體Th1/Th2水平顯著高于健康對照組(0.8089 ±0.2458、0.8993±0.3515、0.8571±0.2470比0.6198±0.1769,均P＜0.01).DN1組胞外體Th 1/Th2顯著高于DM組(P＜0.01).尿胞外體IFN-γ/IL-4與UACR(r=0.213,P=0.015)、BUN( r=0.292,P=0.001)呈正相關.逐步多元迴歸分析顯示,BUN是尿胞外體IFN-γ/IL-4的獨立影響因素(β=0.246,P=0.006).結論 尿胞外體Th1/Th2漂移與2型糖尿病腎病密切相關,可能在糖尿病早期腎病髮病過程中起重要的作用.
목적 탐토2형당뇨병(DM)환자뇨포외체1형보조성T세포/2형보조성T세포(Th1/Th2)적변화여2형당뇨병신병(DN)발생발전적상관성.방법 선취120례2형당뇨병환자급건강대조조30례위대상,근거뇨백단백기항비(UACR),2형당뇨병환자분위당뇨병비신병조(DM,40례,UACR＜30 mg/gCr)、미량백단백뇨조(DN1,50례,UACR≥30 ～300mg/gCr)화림상백단백뇨조(DN2,30례,UACR＞300 mg/gCr).용특이성단극륭항체제순뇨포외체.용매련면역흡부법( ELISA)검측뇨포외체간우소γ(IFN-γ)화백세포개소4(IL-4)수평.용다원축보회귀방법분석뇨포외체IFN-γ/IL-4비치여당화혈홍단백(HbA1c)、담고순(CH)、UACR、혈기항(Scr)、뇨소담(BUN)상관성.결과 DM、DN1、DN2조포외체Th1/Th2수평현저고우건강대조조(0.8089 ±0.2458、0.8993±0.3515、0.8571±0.2470비0.6198±0.1769,균P＜0.01).DN1조포외체Th 1/Th2현저고우DM조(P＜0.01).뇨포외체IFN-γ/IL-4여UACR(r=0.213,P=0.015)、BUN( r=0.292,P=0.001)정정상관.축보다원회귀분석현시,BUN시뇨포외체IFN-γ/IL-4적독립영향인소(β=0.246,P=0.006).결론 뇨포외체Th1/Th2표이여2형당뇨병신병밀절상관,가능재당뇨병조기신병발병과정중기중요적작용.
Objective To examine the correlation of imbalance of urinary exosome Th1/Th2 with diabetic nephropathy (DN).Methods A total of 120 patients with type 2 diabetes mellitus (DM) and 30 healthy volunteers as control were enrolled in the study.According to urinary albumin/creatinine ratio (UACR),type 2 diabetes mellitus patients were divided into 3 groups:diabetes mellitus without nepbropathy group (DM,n=40,UACR＜30 mg/gCr),microalbuminuria group (DN 1,n=50,UACR-30～300 mg/gCr) and clinicoalbuminuria group (DN 2,n=30,UACR＞300 mg/gCr).Urine exosome-interferon-gamma (IFN-γ) and exosome-interleukin 4 (IL-4) levels were determined by enzyme-linked immunosorbent assay (ELISA).Multiple stepwise linear regression was used to analyze the correlation of exosome-IFN-γ/IL-4 with glycated hemoglobin (HbA1c),cholesterol (CH),UACR,Scr and BUN.Results Th1/Th2 ratio in DM,DN1,DN2 groups was significantly higher than that in healthy group (0.8089±0.2458,0.8993 ±0.3515,0.8571±0.2470 vs 0.6198±0.1769,all P＜0.01).Correlation analysis showed that urinary exosomeIFN-γ/IL-4 ratio was positively correlated with UACR (r=0.213,P=0.015) and BUN (r=0.292,P=0.001).Multiple stepwise linear regression analysis showed that BUN was independent determinants for exosome-IFN-γ/IL-4 (β=0.246,P=0.006).Conclusion The imbalance of urinary exosomeTh1/Th2 is correlated with DN,which may play an important role in the pathogenesis of DN.
