CAJ | 학술논문

目的探讨组织间Duffy抗原/趋化因子受体(DARC)在下肢静脉高压炎症反应中的作用及其可能的机制.方法采用股动-静脉瘘建立大鼠后肢静脉高压模型,36只SD大鼠数字随机分为pcDNA3.1-DARC质粒组、pcDNA3.1空质粒组和对照组.术后14 d和42 d,采用实时定量聚合酶链反应(Real-PCR)和蛋白印迹(Western blotting)检测组织间DARC mRNA和蛋白的表达；酶联免疫吸附测定法检测血清白细胞介素(IL)-8的含量；TUNEL法和HE染色检测组织凋亡和白细胞浸润情况.结果随着静脉高压时间的延长,组织间DARC表达量、组织凋亡及白细胞浸润程度在pcDNA3.1-DARC质粒组和pcDNA3.1空质粒组均呈增加的趋势,同期各组间比较,pcDNA3.1-DARC质粒组明显高于pcDNA3.1空质粒组和对照组,差异均有统计学意义(均P＜0.05)；血清IL-8含量在pcDNA3.1-DARC质粒组和pcDNA3.1空质粒组均表现为降低的趋势,同期各组间比较,pcDNA3.-DARC质粒组明显低于pcDNA3.1空质粒组,但两组均高于对照组,差异均有统计学意义(均P＜0.05).结论组织间DARC表达与静脉高压组织的炎症程度呈正相关,可能通过加强白细胞的黏附和迁移促进静脉高压炎症反应的发展.
목적 탐토조직간Duffy항원/추화인자수체(DARC)재하지정맥고압염증반응중적작용급기가능적궤제.방법 채용고동-정맥루건립대서후지정맥고압모형,36지SD대서수자수궤분위pcDNA3.1-DARC질립조、pcDNA3.1공질립조화대조조.술후14 d화42 d,채용실시정량취합매련반응(Real-PCR)화단백인적(Western blotting)검측조직간DARC mRNA화단백적표체；매련면역흡부측정법검측혈청백세포개소(IL)-8적함량；TUNEL법화HE염색검측조직조망화백세포침윤정황.결과 수착정맥고압시간적연장,조직간DARC표체량、조직조망급백세포침윤정도재pcDNA3.1-DARC질립조화pcDNA3.1공질립조균정증가적추세,동기각조간비교,pcDNA3.1-DARC질립조명현고우pcDNA3.1공질립조화대조조,차이균유통계학의의(균P＜0.05)；혈청IL-8함량재pcDNA3.1-DARC질립조화pcDNA3.1공질립조균표현위강저적추세,동기각조간비교,pcDNA3.-DARC질립조명현저우pcDNA3.1공질립조,단량조균고우대조조,차이균유통계학의의(균P＜0.05).결론 조직간DARC표체여정맥고압조직적염증정도정정상관,가능통과가강백세포적점부화천이촉진정맥고압염증반응적발전.
Objective To explore the role and mechanism of Duffy antigen receptor for chemokines (DARC) of tissue in promoting the inflammatory reaction of the limb with venous hypertension.Methods Femoral arteriovenous fistula was surgically created to establish the rat model of venous hypertension.A total of 36 SD rats were randomly divided into pcDNA3.1-DARC ( Group A),empty plasmid of pcDNA3.1 (Group B) and control (Group C) groups.The animals were sacrificed at Days 14 and 42 post-operation respectively.The expressions of DARC at the RNA and protein level were detected by real-time polymerase chain reaction (Real-PCR) and Western blotting.And the serum level of interleukin (IL)-8 was detected by enzyme linked immunosorbent assay (ELISA) and the degrees of apoptosis and leukocytic infiltration of local tissue were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and hematoxylin and eosin (HE) staining.Results With the elapsing time of venous hypertension,the DARC expression in tissue,the extent of apoptosis and leukocytic infiltration in tissue showed an increasing trend in Groups A and B.Group A was obviously higher than Group B during the corresponding period.And the differences were statistically significant (P ＜ 0.05).The serum levels of IL-8 of Groups A and B showed a decreasing trend.And Group A was obviously lower than Group B. Both groups were higher than the control group. The differences were statistically significant ( P ＜ 0.05 ).Conclusion The level of DARC in tissue and the degree of inflammatory reaction of venous hypertension have a positive correlation.And DARC may promote the development of venous hypertension inflammation through augmenting the adhesion and migration of leukocytes.