CAJ | 학술논문

目的以酯键链接增强基团,制备姜黄素前体,观察其对前列腺癌细胞和正常二倍体细胞生长活性影响的差异.方法叔丁氧羰基(Boc)-苯丙氨酸酯姜黄素单酯作用于17组人前列腺癌DU-145细胞6～24h后,噻唑蓝(MTT)药物敏感实验检测细胞生长活性,流式细胞术检测细胞凋亡和比率,透射电镜观察细胞超微结构改变.1 ～7d后,采用计数法测定细胞生长曲线.同法作用于入主动脉平滑肌(HASMC)细胞,作为对照组.结果 10 ～40μmol/L的Boc-苯丙氨酸酯姜黄素单酯作用于人前列腺癌DU-145细胞6～24h后,DU-145细胞生长抑制率为7.37％～66.87％ (P＜0.05),呈浓度、时间依赖性；部分细胞出现凋亡形态学改变,FSC-SSC散点图可见24 h DU-145细胞凋亡比率分别为37.84％～47.12％ (P ＜0.05).对照组HASMC细胞凋亡比率为0.94％～4.23％(P＜0.05),较同浓度姜黄素降低.结论姜黄素前体化合物能在体外有效诱导人前列腺癌DU-145细胞凋亡,对正常二倍体细胞的抑制作用较低.
목적 이지건련접증강기단,제비강황소전체,관찰기대전렬선암세포화정상이배체세포생장활성영향적차이.방법 숙정양탄기(Boc)-분병안산지강황소단지작용우17조인전렬선암DU-145세포6～24h후,새서람(MTT)약물민감실험검측세포생장활성,류식세포술검측세포조망화비솔,투사전경관찰세포초미결구개변.1 ～7d후,채용계수법측정세포생장곡선.동법작용우입주동맥평활기(HASMC)세포,작위대조조.결과 10 ～40μmol/L적Boc-분병안산지강황소단지작용우인전렬선암DU-145세포6～24h후,DU-145세포생장억제솔위7.37％～66.87％ (P＜0.05),정농도、시간의뢰성；부분세포출현조망형태학개변,FSC-SSC산점도가견24 h DU-145세포조망비솔분별위37.84％～47.12％ (P ＜0.05).대조조HASMC세포조망비솔위0.94％～4.23％(P＜0.05),교동농도강황소강저.결론 강황소전체화합물능재체외유효유도인전렬선암DU-145세포조망,대정상이배체세포적억제작용교저.
Objective To prepare curcumin precursors by toughening genes with ester key,and observe their effects on the growth viability of prostate cancer cells and the normal diploid cells.Methods Seventeen groups of human prostatic cancer DU-145 cells were cultured and incubated with Boc-phenylalanine-curcumin (BPC) for 6-24 h.Cell viability was detected by methyl thiazol tetrazolium (MTT).Cell apoptosis and apoptosis rate were examined by using flow cytometry.Ultrastructural changes of cells were observed under the electronic microscopy.Human prostatic cancer DU-145 cells were cultured and incubated with BPC for 1-7 days.Cell viability was tested by counting process.Human aortic smooth muscle cells (HASMC) served as controls.Results After treatment with 10-40 μmol/L BPC for 6-24 h,the growth inhibition rate of human prostatic cancer DU-145 cells was 7.37％-66.87％ (P＜0.05),and the effects were dose- and time-dependent.Partial cancer cells presented morphological changes of apoptosis.After treatment with 10-40 μmol/L BPC for for 24 h,apoptosis rate was 37.84％ -47.12％ (P ＜ 0.05 ).The apoptosis rate in controls was 0.94％ -4.23％ (P ＜ 0.05).Conclusion The prodrug of curcumin could induce apoptosis of prostatic cancer DU-145 cells,and reduce the side effects on normal diploid cells.