CAJ | 학술논문

AtLH基因是BcpLH基因在拟南芥(Arapsis thaliana L.)中的同源基因,含有两个编码双链RNA结合蛋白的结构域.在大白菜叶球发育过程中,BcpLH基因与包叶的卷曲有关.为研究AtLH的基因对叶卷曲这一重要生物学现象的调控作用,构建了35S:AtLH基因的正义表达载体并转化拟南芥.与野生型比较页言,转基因植株的花和叶中AtLH的表达量有显著增加,成为AtLH基因过量的植株.这些植株的莲座叶向外或向下卷曲,呈现明显的偏上性生长;而且抽苔和开花时间延迟;在营养生长期其短缩茎的叶腑处着生数个侧茎,表现为顶端优势减弱;在生殖生长期二级花序减少使得主花序更加发达,表现为顶端优势增强,转基因植株对激素的敏感性改变,IAA剌激根生长的作用增强,ABA抑制根生长的作用减弱.由此可见,AtLH基因的过量表达可引起转基因植株的叶片向下卷曲.
AtLH기인시BcpLH기인재의남개(Arapsis thaliana L.)중적동원기인,함유량개편마쌍련RNA결합단백적결구역.재대백채협구발육과정중,BcpLH기인여포협적권곡유관.위연구AtLH적기인대협권곡저일중요생물학현상적조공작용,구건료35S:AtLH기인적정의표체재체병전화의남개.여야생형비교혈언,전기인식주적화화협중AtLH적표체량유현저증가,성위AtLH기인과량적식주.저사식주적련좌협향외혹향하권곡,정현명현적편상성생장;이차추태화개화시간연지;재영양생장기기단축경적협부처착생수개측경,표현위정단우세감약;재생식생장기이급화서감소사득주화서경가발체,표현위정단우세증강,전기인식주대격소적민감성개변,IAA랄격근생장적작용증강,ABA억제근생장적작용감약.유차가견,AtLH기인적과량표체가인기전기인식주적협편향하권곡.
AtLH gene of Arabidopsis is a BcpLH(leafy head) homolog of Chinese cabbage, which encodes a double-stranded RNA-binding protein related to the curvature of folding leaf leading to the formation of leafy head. In order to elucidate the regulatory function of AtLHin the development of leaf curvature, we made a construct of 35S::AtLH and transformed it to Arabidopsis. In transgenic plants for sense-AtLH,transcripts of AtLH gene were increased significantly in leaves and flowers, giving rise to the AtLHoverexpressed plants in which the rosette leaves curved downward or outward in a manner of enhanced epinastic growth. Compared with normal plants, bolting and flowering time of the transgenic plants was significantly delayed. Moreover, the apical dominance of transgenic plants was weaker in vegetative shoots since more axillary shoots emerged from axil of rosette leaves, while stronger in flowering shoots because fewer cauline inflorescences were observed on the main inflorescence. In other aspects, these transgenic plants exhibited an increase in root-stimulating response to IAA and decrease in root-inhibitory reaction on ABA. It indicates that overexpression of AtLH causes downward curvature of transgenic plants.