CAJ | 학술논문

选取三峡库区自然消落带野生狗牙根(XC)和非消落带野生狗牙根(FC)为研究对象,采用三重复裂区设计试验,主区为两品种,副区为6个不同深度水淹处理(0,1,2,5,10m和15m),分析了各处理植株几种酶活性和碳水化合物含量的变化情况.结果显示:①不同生境狗牙根受深淹胁迫后,丙二醛(MDA)都呈递增趋势,表明狗牙根在深淹胁迫下受到了不同程度的膜脂过氧化伤害,且随胁迫程度的增加受伤害增大.②超氧化物歧化酶(SOD)、过氧化物酶(POD)、谷胱甘肽还原酶(GR)活性和醇脱氢酶(ADH)活性较对照都有所增加;XC处理POD和SOD的最大值出现在5m深水淹处理,而FC出现在2m深处理,且XC 5米深淹处理GR值较对照和其他处理明显大;各受淹处理地下茎可溶性糖含量和淀粉含量都保持在较高水平.③从抗氧化酶活性和ADH活性的变化规律,以及淹水胁迫下植株的能量代谢情况,初步得出XC与FC都具有一定的耐淹性,且XC较FC具更耐深淹的能力,表明狗牙根作为禾本科植物遗传上具有一定的耐淹性,自然消落带狗牙根因长期生长于水淹胁迫环境,耐淹能力得到了进一步强化.从狗牙根对深淹胁迫的生理响应上,证明狗牙根是宜用于三峡水库消落带植被恢复的物种.
선취삼협고구자연소락대야생구아근(XC)화비소락대야생구아근(FC)위연구대상,채용삼중복렬구설계시험,주구위량품충,부구위6개불동심도수엄처리(0,1,2,5,10m화15m),분석료각처리식주궤충매활성화탄수화합물함량적변화정황.결과현시:①불동생경구아근수심엄협박후,병이철(MDA)도정체증추세,표명구아근재심엄협박하수도료불동정도적막지과양화상해,차수협박정도적증가수상해증대.②초양화물기화매(SOD)、과양화물매(POD)、곡광감태환원매(GR)활성화순탈경매(ADH)활성교대조도유소증가;XC처리POD화SOD적최대치출현재5m심수엄처리,이FC출현재2m심처리,차XC 5미심엄처리GR치교대조화기타처리명현대;각수엄처리지하경가용성당함량화정분함량도보지재교고수평.③종항양화매활성화ADH활성적변화규률,이급엄수협박하식주적능량대사정황,초보득출XC여FC도구유일정적내엄성,차XC교FC구경내심엄적능력,표명구아근작위화본과식물유전상구유일정적내엄성,자연소락대구아근인장기생장우수엄협박배경,내엄능력득도료진일보강화.종구아근대심엄협박적생리향응상,증명구아근시의용우삼협수고소락대식피회복적물충.
A submergence experiment was conducted on Bermudagrasses originally distributed in the water-level-fluctuation zone (XC) and non-water-level-fluctuation zone (FC) of the Three Gorges Reservoir Area. Six treatments with respective submerged depth of 0, 1, 2, 5, 10, 15m were carried out to investigate the physiological responses of Bermudagrasses. The results indicated that: ①Malondialdehyde (MDA) content increased significantly with the increase of submergence depth,, indicating that Bermudagrass suffered the damage of membrane lipid peroxidation under the submergence condition;. ②The activities of superoxide dismutase (SOD), peroxide (POD), glutathione reductase (GR) and alcohol dehydrogenase (ADH) in the roots of Bermudagrass were higher than that of un-submerged control. The maximum of POD, SOD and GR activities of XC appeared at 5 meters in submergence depth, and FC at 2 meters. There were relative higher soluble sugar and starch contents by the submergence treatment. ③Bermudagrasses originally growing in the natural water-level-fluctuation zone (XC) could endure much deeper submergence than FC by the characteristics of antioxidative enzymatic activities, ADH activity and energy metabolism. The results implies that Bermudagrass has submergence-tolerant instinct, and the submergence-tolerant ability of XC was strengthened by the long-time periodic submergence in its natural habitats. Bermudagrass could be a promising species for revegetation of the water-level-fluctuation zone in the Three Gorges Reservoir Area.