中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2010年
1期
36-39
,共4页
薛丽英%张祥宏%李学民%李月红%丁涛%米建民%王俊灵%严霞%温实%邢欣%崔晋峰
薛麗英%張祥宏%李學民%李月紅%丁濤%米建民%王俊靈%嚴霞%溫實%邢訢%崔晉峰
설려영%장상굉%리학민%리월홍%정도%미건민%왕준령%엄하%온실%형흔%최진봉
食管鳞状细胞癌%CD40%COX-2%微血管密度
食管鱗狀細胞癌%CD40%COX-2%微血管密度
식관린상세포암%CD40%COX-2%미혈관밀도
Esophageal squamous cell carcinoma%CD40%COX-2%MVD
目的:探讨CD40和COX-2在食管鳞状细胞癌组织中的表达情况、临床病理意义及其与肿瘤血管生成的关系.方法:采用免疫组织化学方法研究79例食管癌和28例正常食管黏膜上皮组织中CD40和COX-2的表达情况,根据CD34表达计算食管癌组织平均微血管密度(MVD);分析CD40、COX-2的表达与食管癌发生部位、肿瘤大小、分化程度、MVD及淋巴结转移的关系.同时采用免疫细胞化学染色及Western Blot方法检测食管癌Ecal09细胞和原代培养的正常食管上皮细胞CD40和COX-2在蛋白水平上表达的差异.结果:免疫组化结果表明.食管癌组织中CD40和COX-2的阳性表达率均明显高于正常食管鳞状上皮(54.43%vs 10.71%,69.62%vs 17.86%,P均<0.05).CD40在食管癌中的表达与淋巴结转移有关,伴有淋巴结转移者CD40阳性表达率明显高于无淋巴结转移者(70.37%vs 46.15%,P<0.05),但CD40表达与其他临床病理特征无明显相关.COX-2在食管癌中的阳性表达与肿瘤大小、部位、分化程度、有无淋巴结转移等均无明显相关(P>0.05).CD40与COX-2在食管癌组织中表达明显相关(P<0.05),相关系数(φ)为0.446.食管癌组织中MVD明显高于正常食管组织(25.02±5.52 vs 12.09±4.55.P<0.05).CD40和COX-2阳性表达组食管癌组织MVD明显高于阴性表达组(26.37±6.02 vs 22.58±5.25,P<0.05).体外研究结果表明,食管癌Eca109细胞CD40和COX-2表达均高于正常食管上皮细胞(P<0.05).结论:CD40表达与食管鳞状细胞癌的发生、发展密切相关,CD40可能通过影响COX-2的表达促进食管癌组织微血管生成.
目的:探討CD40和COX-2在食管鱗狀細胞癌組織中的錶達情況、臨床病理意義及其與腫瘤血管生成的關繫.方法:採用免疫組織化學方法研究79例食管癌和28例正常食管黏膜上皮組織中CD40和COX-2的錶達情況,根據CD34錶達計算食管癌組織平均微血管密度(MVD);分析CD40、COX-2的錶達與食管癌髮生部位、腫瘤大小、分化程度、MVD及淋巴結轉移的關繫.同時採用免疫細胞化學染色及Western Blot方法檢測食管癌Ecal09細胞和原代培養的正常食管上皮細胞CD40和COX-2在蛋白水平上錶達的差異.結果:免疫組化結果錶明.食管癌組織中CD40和COX-2的暘性錶達率均明顯高于正常食管鱗狀上皮(54.43%vs 10.71%,69.62%vs 17.86%,P均<0.05).CD40在食管癌中的錶達與淋巴結轉移有關,伴有淋巴結轉移者CD40暘性錶達率明顯高于無淋巴結轉移者(70.37%vs 46.15%,P<0.05),但CD40錶達與其他臨床病理特徵無明顯相關.COX-2在食管癌中的暘性錶達與腫瘤大小、部位、分化程度、有無淋巴結轉移等均無明顯相關(P>0.05).CD40與COX-2在食管癌組織中錶達明顯相關(P<0.05),相關繫數(φ)為0.446.食管癌組織中MVD明顯高于正常食管組織(25.02±5.52 vs 12.09±4.55.P<0.05).CD40和COX-2暘性錶達組食管癌組織MVD明顯高于陰性錶達組(26.37±6.02 vs 22.58±5.25,P<0.05).體外研究結果錶明,食管癌Eca109細胞CD40和COX-2錶達均高于正常食管上皮細胞(P<0.05).結論:CD40錶達與食管鱗狀細胞癌的髮生、髮展密切相關,CD40可能通過影響COX-2的錶達促進食管癌組織微血管生成.
목적:탐토CD40화COX-2재식관린상세포암조직중적표체정황、림상병리의의급기여종류혈관생성적관계.방법:채용면역조직화학방법연구79례식관암화28례정상식관점막상피조직중CD40화COX-2적표체정황,근거CD34표체계산식관암조직평균미혈관밀도(MVD);분석CD40、COX-2적표체여식관암발생부위、종류대소、분화정도、MVD급림파결전이적관계.동시채용면역세포화학염색급Western Blot방법검측식관암Ecal09세포화원대배양적정상식관상피세포CD40화COX-2재단백수평상표체적차이.결과:면역조화결과표명.식관암조직중CD40화COX-2적양성표체솔균명현고우정상식관린상상피(54.43%vs 10.71%,69.62%vs 17.86%,P균<0.05).CD40재식관암중적표체여림파결전이유관,반유림파결전이자CD40양성표체솔명현고우무림파결전이자(70.37%vs 46.15%,P<0.05),단CD40표체여기타림상병리특정무명현상관.COX-2재식관암중적양성표체여종류대소、부위、분화정도、유무림파결전이등균무명현상관(P>0.05).CD40여COX-2재식관암조직중표체명현상관(P<0.05),상관계수(φ)위0.446.식관암조직중MVD명현고우정상식관조직(25.02±5.52 vs 12.09±4.55.P<0.05).CD40화COX-2양성표체조식관암조직MVD명현고우음성표체조(26.37±6.02 vs 22.58±5.25,P<0.05).체외연구결과표명,식관암Eca109세포CD40화COX-2표체균고우정상식관상피세포(P<0.05).결론:CD40표체여식관린상세포암적발생、발전밀절상관,CD40가능통과영향COX-2적표체촉진식관암조직미혈관생성.
Objective:To explore the putative role of CD40 and COX-2 expression in human esophageal squamous cell carcinoma(ESCC)and to analyze their possible relationship with angiogenesis in ESCC.Methods:The expression of CD40 and COX-2 was detected in 79 ESCC and 28 normal esophageal epithelial tissue samples with immunohistochemical staining.The microvessel density by CD34 was determined and the clinicopathological significance of CD40 and COX-2 expression in ESCC was analyzed.In addition,the expression of CD40 and COX-2 in esophageal squamous cell carcinoma cell line Eca109 and primary cultured normal esophageal epithelial cells in vivo was comparatively studied with immunocytochemical staining andWestern blot.Results:Compared with that in normal epithelial tissues,the expression of CD40 and COX-2 in ESCC was significantly higher(54.43%vs 10.71%:69.62%vs 17.86%:respectively,P<0.05).The positive expression rate of CD40 in ESCC cases with lymph node metastasis was significantly higher than that in those without lymph node metastasis(70.37%vs 46.1 5%.P<0.05).No correlation was found between CD40 expression and patient age,sex,tumor location,size and differentiation of tumors.No clinicopathological significanca of COX-2 expression in ESCC was found.There was a positive correlation between CD40 expression and COX-2 expression in esophageal squamous cell carcinoma(P<0.05,φ=0.446).The mean MVD value in ESCC was significantly higher than that in normal esophageal tissue(25.02±5.52 vs 12.09±4.55,P<0.05).MVD value in ESCC was closely correlated with lymph node metastasis.The mean MVD value in ESCC cases with positive CD40 and COX-2 expression was higher than that in those with negative CD40 and COX-2expression(26.37±6.02 vs 22.58±5.25.P<0.05).Western blot results showed that CD40 and COX-2 expression in Eca109 was higher than that in cultured normal esophageal epithelial cells (P<0.05).Conclusion:The expression of CD40 is involved in the carcinogenesis and progression of esophageal squamous cell carcinoma.CD40 may play an important role in the angiogenesis of ESCC through promoting COX-2 expression.
