CAJ | 학술논문

[目的]研究陕西祖师麻药材化学成分HPLC指纹图谱,为科学评价、有效控制药材质量提供可靠方法.[方法]采用VP ODS C_(18)(4.6 mm×250.0 mm,5μm)色谱柱,甲醇-水(0.02%磷酸),梯度洗脱,流速1.0 ml/min,检测波长326 nm,柱温30 ℃,进样量10 μl,测定祖师麻药材HPLC指纹图谱.[结果]根据聚类分析结果,选用10批陕西产祖师麻药材,建立了祖师麻药材指纹图谱共有模式,显示13个共有峰,指认了祖师麻甲素、紫丁香苷 2个特征峰.[结论]HPLC指纹图谱中各峰分离度较好,特征明显,方法的精密度、重现性试验的RSD值小于5%,可作为陕西祖师麻药材质量控制标准.
[목적]연구협서조사마약재화학성분HPLC지문도보,위과학평개、유효공제약재질량제공가고방법.[방법]채용VP ODS C_(18)(4.6 mm×250.0 mm,5μm)색보주,갑순-수(0.02%린산),제도세탈,류속1.0 ml/min,검측파장326 nm,주온30 ℃,진양량10 μl,측정조사마약재HPLC지문도보.[결과]근거취류분석결과,선용10비협서산조사마약재,건립료조사마약재지문도보공유모식,현시13개공유봉,지인료조사마갑소、자정향감 2개특정봉.[결론]HPLC지문도보중각봉분리도교호,특정명현,방법적정밀도、중현성시험적RSD치소우5%,가작위협서조사마약재질량공제표준.
[Objective] The research aimed to study HPLC fingerprints of Zushima in Shaanxi Province for offering a method on the quality control and assessment.[Method] HPLC fingerprints of Zushima were established. Separations and determinations were carried out by linear gradient elution under following conditions:VP ODS C_(18) column (4.6 mm×250.0 mm,5 μm), methanol -0.02% phosphoric acid solution as mobile phase, detection wavelength of UV at 326 nm, flow rate of 1.0 ml/min, column temperature of 30 ℃. [Result] Based on the result of clustering analysis, 10 samples of Zushima in Shaanxi Province were used to evaluate the whole HPLC spectrums. 13 common peaks were identified as the characteristic fingerprints of Zushima. [Conclusion]The established HPLC fingerprints had desirable precision, producibility, stability, and could be used for the quality control of Zushima in Shaanxi Province.