CAJ | 학술논문

为了解猪Toll样受体5(TLR5)蛋白的结构特征和进化关系,本研究从肠系膜淋巴结组织总RNA中克隆出猪Toll样受体5基因的cDNA序列.序列全长2 641 bp,其中2 571 bp的开放阅读框编码856个氨基酸残基的猪TLR5,含17.4%的亮氨酸,并有一段19个氨基酸的信号肽序列;同源性分析结果显示,TLR5在进化过程中具有高度保守性,与人、牛、山羊、绵羊、小鼠和大鼠的氨基酸序列同源性分别为77.5%、79.6%、78.3%、81.0%、69.2%和68.9%.蛋白分子结构预测结果表明,该分子由胞外区(642个氨基酸)、跨膜区(23个氨基酸)和胞内区(191个氨基酸)组成,胞外区具有LRR结构域,胞内区具有TIR结构域,表现出典型的TLR家族结构特征.表明猪TLR5分子具有病原分子模式识别和信号传导的作用,这为其结构与功能的进一步研究奠定了基础.
위료해저Toll양수체5(TLR5)단백적결구특정화진화관계,본연구종장계막림파결조직총RNA중극륭출저Toll양수체5기인적cDNA서렬.서렬전장2 641 bp,기중2 571 bp적개방열독광편마856개안기산잔기적저TLR5,함17.4%적량안산,병유일단19개안기산적신호태서렬;동원성분석결과현시,TLR5재진화과정중구유고도보수성,여인、우、산양、면양、소서화대서적안기산서렬동원성분별위77.5%、79.6%、78.3%、81.0%、69.2%화68.9%.단백분자결구예측결과표명,해분자유포외구(642개안기산)、과막구(23개안기산)화포내구(191개안기산)조성,포외구구유LRR결구역,포내구구유TIR결구역,표현출전형적TLR가족결구특정.표명저TLR5분자구유병원분자모식식별화신호전도적작용,저위기결구여공능적진일보연구전정료기출.
In this study porcine toll-like receptor5 (TLR5) gene was cloned from porcine mesenterie lymph nodes (MLNs) by RT-PCR. Sequence analysis showed that the porcine TLR5 cDNA was 2 641 bp in length with an open reading frame encoding 856 amino acids residues, including 17.4 % leucines and a signal peptide of 19 amino acids. The nucleotide sequence of the gene shared 99.7 % identities with the published sequence. The amino acids sequence shared 77.5%, 79.6%, 78.3%, 81.0%, 69.2% and 68.9% homology with that of human, cattle, goat, sheep, mouse and rat, respectively. The structure prediction showed that the porcine TLR5 is a typical type Ⅰ transmembrane protein composed of extracellular, transmembrane and intracellular regions, which contain leucine-rich repeats (LRR) eetodomains and Toll/IL-IR domain (TIR) cytoplsmic domains. These results indicated that the porcine TLR5 could play a crucial role in recognition of pathogen-associated molecular patterns and signal transduction.