CAJ | 학술논문

通过分析低氧环境下真鲷（P.major♀）×黑鲷（S.macrocephalus♂）杂交子一代体内酶活性的变化评价其耐低氧能力.将杂交子一代置于溶氧量为（1.83±0.27）mg.L-1的水体中,胁迫0、6、12和24h后,取其肝脏、鳃和肌肉检测乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和总抗氧化能力（T-AOC）.结果显示：低氧胁迫后LDH活性在肌肉和肝脏中分别升高25.7%和17.6%;脏中SOD活性升高20.2%,随胁迫时间延长活性下降至初始水平;CAT活性下降44.3%～62.4%;T-AOC活力也呈先升后降趋势.说明杂交子一代具有一定的低氧耐受能力,但长时间低氧胁迫会对其造成氧化损伤.
통과분석저양배경하진조（P.major♀）×흑조（S.macrocephalus♂）잡교자일대체내매활성적변화평개기내저양능력.장잡교자일대치우용양량위（1.83±0.27）mg.L-1적수체중,협박0、6、12화24h후,취기간장、새화기육검측유산탈경매（LDH）、초양화물기화매（SOD）、과양화경매（CAT）활성화총항양화능력（T-AOC）.결과현시：저양협박후LDH활성재기육화간장중분별승고25.7%화17.6%;장중SOD활성승고20.2%,수협박시간연장활성하강지초시수평;CAT활성하강44.3%～62.4%;T-AOC활력야정선승후강추세.설명잡교자일대구유일정적저양내수능력,단장시간저양협박회대기조성양화손상.
To examine hypoxia-induced stress at metabolic and antioxidant enzyme levels,we exposed the P.major♀×S.macrocephalus♂ F1 to（1.83±0.27） mg·L-1 to the dissolved oxygen.After 0,6,12 and 24 h exposure,samples of liver,gill,and muscle tissue are extracted and subsequently analyzed to determine the lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,catalase（CAT）,and total anti-oxidative capability（T-AOC）.Hypoxia facilitates increasing the LDH content in the muscle（25.7%） and liver（17.6%）.The activity of SOD returns to control level after an initial increase（20.2%） in liver.Hypoxia decreases the CAT content in the liver（44.3%～62.4%）.T-AOC activity significantly increases in the liver（33.8%～54.1%） and gill（239%）,but decreases during longer hypoxia exposure.The results show that the hybrid has a certain degree of hypoxia tolerance,but its long hypoxia stress may cause oxidative damage.