CAJ | 학술논문

目的探讨外周血CD+4CDhigh25CDlow127调节性T细胞(Treg细胞)水平与非霍奇金淋巴瘤(NHL)的关系.方法采用流式细胞术检测123例初治NHL患者、41名健康成年人外周血CD+4CDhigh25CDlow127 Treg水平,并分析其与临床因素的关系.结果健康成年人外周血CD+4CDhigh25CDlow127 Treg水平中位数为5.0％ (四分位数间距为2.7％),初诊NHL患者中位数为7.2％(四分位数间距为3.8％),两者之间差异有统计学意义(Z=-2.816,P=0.001).初诊NHL患者外周血Treg水平在男性患者中中位数为7.2％(四分位数间距为7.4％),在女性患者中中位数为6.1％(四分位数间距为3.8％),差异有统计学意义(Z=- 2.729,P=0.006)；乳酸脱氢酶(LDH)增高组中位数为7.8％(四分位数间距为5.9％),LDH正常组中位数为7.0％(四分位数间距为3.3％),差异具有统计学意义(Z=- 2.623,P=0.008)；Ⅲ～Ⅳ期患者中位数为7.3％(四分位数间距为53％),Ⅰ ～Ⅱ期患者中位数为5.9％(四分位数间距为3.7％),差异有统计学意义(Z=- 2.057,p=0.04)；而NHL患者在不同年龄、IPI评分、结外病变、体能状况、大包块、病理类型、B症状等之间外周血Treg水平差异无统计学意义(均P＞0.05).结论 NHL患者体内存在免疫抑制,在男性患者、LDH增高患者及分期为Ⅲ～Ⅳ期患者中CD+4CDhigh25CDlow127 Treg水平明显增高.
목적 탐토외주혈CD+4CDhigh25CDlow127조절성T세포(Treg세포)수평여비곽기금림파류(NHL)적관계.방법 채용류식세포술검측123례초치NHL환자、41명건강성년인외주혈CD+4CDhigh25CDlow127 Treg수평,병분석기여림상인소적관계.결과 건강성년인외주혈CD+4CDhigh25CDlow127 Treg수평중위수위5.0％ (사분위수간거위2.7％),초진NHL환자중위수위7.2％(사분위수간거위3.8％),량자지간차이유통계학의의(Z=-2.816,P=0.001).초진NHL환자외주혈Treg수평재남성환자중중위수위7.2％(사분위수간거위7.4％),재녀성환자중중위수위6.1％(사분위수간거위3.8％),차이유통계학의의(Z=- 2.729,P=0.006)；유산탈경매(LDH)증고조중위수위7.8％(사분위수간거위5.9％),LDH정상조중위수위7.0％(사분위수간거위3.3％),차이구유통계학의의(Z=- 2.623,P=0.008)；Ⅲ～Ⅳ기환자중위수위7.3％(사분위수간거위53％),Ⅰ ～Ⅱ기환자중위수위5.9％(사분위수간거위3.7％),차이유통계학의의(Z=- 2.057,p=0.04)；이NHL환자재불동년령、IPI평분、결외병변、체능상황、대포괴、병리류형、B증상등지간외주혈Treg수평차이무통계학의의(균P＞0.05).결론 NHL환자체내존재면역억제,재남성환자、LDH증고환자급분기위Ⅲ～Ⅳ기환자중CD+4CDhigh25CDlow127 Treg수평명현증고.
Objective To explore the relationship between CD+4CDhigh25CDlow127 Treg level of peripheral blood and NHL.Methods The level of peripheral blood CD+4CDhigh25CDlow127 Treg of 123 newly diagnosed NHL and 41 healthy adults were test by flow cytometry.Results The average peripheral blood CD+4CDhigh25CDlow127 Treg level were 5.0 ％ (2.7 ％) and 7.2 ％(3.8 ％) in healthy adults and newly diagnosed NHL patients,respectively.The difference between the two groups was statistically significant(Z=-2.816 P=0.001 ).The peripheral blood Treg level was significantly higher in the male NHL patients than that in the female (7.4％ vs 6.1％,Z=- 2.729,P=0.006).The peripheral blood Treg level was significantly higher in elevated LDH patients than that in normal LDH patients(7.8％ vs 7.0％,Z=-2.623 P=0.008).The peripheral blood Treg level was significantly higher in stage Ⅲ-Ⅳ patients than that in stage Ⅰ -Ⅱ patients (7.3％ vs 5.9％,Z=-2.057 P=0.04). There was no significant relationship between peripheral blood Treg level and age, IPI score,extranodal involvement,performance status,bulky disease,pathologic subtype or B symptom (P ＞0.05).Conclusions NHL patients are in an immunosuppresive statue.Patients of male,with inreased LDH level or in stage Ⅲ-Ⅳ have higher peripheral blood CD+4CDhigh25CDlow127 Treg level.