CAJ | 학술논문

目的探讨CYP3A5、MDR1和COX-2单核苷酸多态性是否与长春瑞滨、铂类药物对晚期非小细胞肺癌(NSCLC)化疗疗效有关.方法对69例采用长春瑞滨、铂类方案化疗的晚期NSCLC患者(中国汉族)的CYP3A5(*3)、MDR1 21外显子上的2677 G>T位点、26外显子3435 C>T位点及其单体型和COX-2启动子区的-1195 G>A位点用限制内切酶片段多态性-多聚酶链反应(RFLP-PCR)的方法进行基因分型,结合临床化疗效果进行分析,采用卡方检验分析各位点基因型与化疗疗效之间的关系.结果 MDR1 3435位点野生纯合子(C/C)患者疗效明显高于杂合子(C/T)和突变纯合子(T/T)患者(P=0.033).MDR1 2677位点野生纯合子(G/G)患者疗效明显高于其他基因型患者(P=0.012).MDR1 2677 G-3435 C单体型患者疗效稍高于其他单体型(P=0.063).CYP3A5*3位点基因型与长春瑞滨、铂类方案疗效无关.COX-2-1195 G>A位点野生型(G/G)疗效稍高于其他基因型(P=0.067),但差异无统计学意义.结论 MDR13435 C>T位点基因型和MDR1 2 677 G>A/T位点基因型可以用于预测长春瑞滨、铂类方案对晚期NSCLC患者的疗效.
목적 탐토CYP3A5、MDR1화COX-2단핵감산다태성시부여장춘서빈、박류약물대만기비소세포폐암(NSCLC)화료료효유관.방법 대69례채용장춘서빈、박류방안화료적만기NSCLC환자(중국한족)적CYP3A5(*3)、MDR1 21외현자상적2677 G>T위점、26외현자3435 C>T위점급기단체형화COX-2계동자구적-1195 G>A위점용한제내절매편단다태성-다취매련반응(RFLP-PCR)적방법진행기인분형,결합림상화료효과진행분석,채용잡방검험분석각위점기인형여화료료효지간적관계.결과 MDR1 3435위점야생순합자(C/C)환자료효명현고우잡합자(C/T)화돌변순합자(T/T)환자(P=0.033).MDR1 2677위점야생순합자(G/G)환자료효명현고우기타기인형환자(P=0.012).MDR1 2677 G-3435 C단체형환자료효초고우기타단체형(P=0.063).CYP3A5*3위점기인형여장춘서빈、박류방안료효무관.COX-2-1195 G>A위점야생형(G/G)료효초고우기타기인형(P=0.067),단차이무통계학의의.결론 MDR13435 C>T위점기인형화MDR1 2 677 G>A/T위점기인형가이용우예측장춘서빈、박류방안대만기NSCLC환자적료효.
Objective To investigate whether genotypes of CYP3A5,MDR1 and cyclooxygenase-2 are associated with the sensitivity of vinorelbine-platinum to NSCLC.Methods The genotypes of CYP3A5(*3),MDR1 (2677G>T at exon 21 and 3435C>T at exon 26 and their haplotypes),cyclooxygenase-2 (-1 195G>A) were determined by RFLP-PCR and chemotherapy responses were analyzed in 69 non-small-celllung cancer (NSCLC) Chinese Han patients.They received a combination chemotherapy of vinorelbine-cispla-tin.Chi-square test was used to investigate the potential association of genotype with chemotherapy response.OR and 95% C1 were calculated.Results The 3435 CC genotype was associated with a significantly betterchemotherapy response compared with the combined 3435 CT and 1Tr genotypes(P=0.033).The 2677 GG genotype was also associated with a significantly better chemotherapy response compared with the combined 2677 GT and IT genotype(P=0.012).Moreover.patients with the 2677 G-3435 C haplotype seemed to have a better response to chemotherapy compared with those with the other haplotypes(P=0.063).CYP3A5*3 was not likely to correlate with sensitivity of vinorelbine-platinum to NSCLC.Cyclooxygenase-2-1195G>A was likely to have better response to vinorelbine but not statistically significant(P=0.067).Conclusion Polymor-Dhisms of MDR1 3435 C>T and MDR1 2677 G>A/T can be used for predicting treatment response to vinorel-bine-cisplatin chemotherapy in NSCLC patients.