CAJ | 학술논문

目的探讨HIF-1α基因沉默对A549肺癌细胞放射敏感性及裸鼠移植瘤生长的影响.方法采用克隆形成实验,评价A549/HIF-1α(－)细胞及A549细胞对X射线的敏感性.将A549/HIF-1α(－)细胞及A549细胞接种于BALB/C雄性裸小鼠,观察肿瘤生长情况.免疫组织化学法检测肿瘤内HIF-1α蛋白的表达及瘤内微血管密度.结果 HIF-1α基因沉默在常氧条件下放射增敏比(SER)为1.06,乏氧条件下为1.65.A549/HIF-1α(－)组移植瘤体积明显小于A549组,A549/HIF-1α(－)组肿瘤细胞HIF-1α蛋白表达显著下降,A549组肿瘤微血管密度为19.83±4.09,而A549/HIF-1α(－)组为11.61±3.04(F=15.57,P＜0.05).结论 HIF-1α基因沉默可以逆转乏氧诱导的A549肺癌细胞对X射线敏感性的降低,并延缓裸鼠移植瘤的生长,使HIF-1α蛋白表达下降和血管生成减少.
목적 탐토HIF-1α기인침묵대A549폐암세포방사민감성급라서이식류생장적영향.방법 채용극륭형성실험,평개A549/HIF-1α(－)세포급A549세포대X사선적민감성.장A549/HIF-1α(－)세포급A549세포접충우BALB/C웅성라소서,관찰종류생장정황.면역조직화학법검측종류내HIF-1α단백적표체급류내미혈관밀도.결과 HIF-1α기인침묵재상양조건하방사증민비(SER)위1.06,핍양조건하위1.65.A549/HIF-1α(－)조이식류체적명현소우A549조,A549/HIF-1α(－)조종류세포HIF-1α단백표체현저하강,A549조종류미혈관밀도위19.83±4.09,이A549/HIF-1α(－)조위11.61±3.04(F=15.57,P＜0.05).결론 HIF-1α기인침묵가이역전핍양유도적A549폐암세포대X사선민감성적강저,병연완라서이식류적생장,사HIF-1α단백표체하강화혈관생성감소.
Objective To observe the radiosensitivity by targeting HIF-lα in human lung cancer and the effects on tumor growth in nude mice.Methods Radiosensitivity of A549 and A549/HIF-1α ( － ) cells were tested by clonogenic forming assay.A549/HIF-1 α( － ) cells and A549 cells were injected into the male BALB/C nude mice.Tumor growth was observed.The expression of HIF-1α and microvessel density were detected by immunohistochemistry method.Results SERs of HIF-1α gene silencing were 1.03 in normoxia and 1.65 in hypoxia.The sizes of tumor xenografts derived from A549/HIF-1α( － ) cells were significantly reduced compared to those of the xenografts derived from A549 cells.HIF-1 α protein staining result showed a dramatic decrease in tumors from A549/HIF-1α ( － ) mice.The microvessel densities (MVD) were 19.83 ± 4.09 in A549 group and 11.61 ±3.04 in A549/HIF-lα (-)group(F=15.57,P ＜0.05 ).Conclusions Hypoxia-induced radio-resistance in lung cancer A549 cells could be reversed by silencing the HIF-1α.It also retards the growth of tumor xenografts,decreases HIF-1α expression and reduces the vascularity.