中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2000年
3期
161-163
,共3页
环孢菌素%抗体,单克隆%免疫耐受
環孢菌素%抗體,單剋隆%免疫耐受
배포균소%항체,단극륭%면역내수
Cyclosporine%Antibodies,monoclonal%Irmnune tolerance
目的 探讨抗原特异性无能T淋巴细胞的诱导及其耐受特性.方法 在体外建立抗原递呈细胞-T淋巴细胞-B淋巴细胞反应系统,利用环孢素A(CsA)与抗B7-1单克隆抗体联用阻断B7:CD28共刺激途径,采用3H-TdR法检测T淋巴细胞增殖,酶联免疫吸附试验(ELISA法)测定B淋巴细胞分泌的抗体.结果 CsA与抗B7-1单克隆抗体联用可诱致T淋巴细胞无能,无能T淋巴细胞不产生白细胞介素2(IL-2);外源性IL-2能阻止T淋巴细胞无能的诱导,但不能逆转这一耐受状态.结论 CsA与抗B7-1单克隆抗体联用可以诱导T淋巴细胞的抗原特异性耐受.
目的 探討抗原特異性無能T淋巴細胞的誘導及其耐受特性.方法 在體外建立抗原遞呈細胞-T淋巴細胞-B淋巴細胞反應繫統,利用環孢素A(CsA)與抗B7-1單剋隆抗體聯用阻斷B7:CD28共刺激途徑,採用3H-TdR法檢測T淋巴細胞增殖,酶聯免疫吸附試驗(ELISA法)測定B淋巴細胞分泌的抗體.結果 CsA與抗B7-1單剋隆抗體聯用可誘緻T淋巴細胞無能,無能T淋巴細胞不產生白細胞介素2(IL-2);外源性IL-2能阻止T淋巴細胞無能的誘導,但不能逆轉這一耐受狀態.結論 CsA與抗B7-1單剋隆抗體聯用可以誘導T淋巴細胞的抗原特異性耐受.
목적 탐토항원특이성무능T림파세포적유도급기내수특성.방법 재체외건립항원체정세포-T림파세포-B림파세포반응계통,이용배포소A(CsA)여항B7-1단극륭항체련용조단B7:CD28공자격도경,채용3H-TdR법검측T림파세포증식,매련면역흡부시험(ELISA법)측정B림파세포분비적항체.결과 CsA여항B7-1단극륭항체련용가유치T림파세포무능,무능T림파세포불산생백세포개소2(IL-2);외원성IL-2능조지T림파세포무능적유도,단불능역전저일내수상태.결론 CsA여항B7-1단극륭항체련용가이유도T림파세포적항원특이성내수.
Objectives To study the induction and tolerance properties of antigen-specific T cell anergy.Methods APC:T:B cell reactive system was established in vitro.T cell proliferation was analyzed by using 3H-TdR incorporation in the blockade of B7/CD28 with B7-1 mAb and CsA.B cell antibody production was assayed by using ELISA Results Antigen-specific T cell anergy can be successfuily induced by the combination of CsA and B7-lmAb in vitro.Anergic T cell can not produce IL-2.but can secrete IL4.Anergic T cells can inhibit MLR.B cell proliferation and specific antibody production.Exogenous IL-2 can prevent T celt anergic induction,but can not reverse the state of tolerance.Conclusions Antigenspecific T cell anergy could be induced by CsA-combined with B7-1 mAb,which would provide theoretical evidence for prevention of transplantation rejection.
