中国医药
中國醫藥
중국의약
CHINA MEDICINE
2008年
8期
449-451
,共3页
心肌缺血再灌注%生长激素%凋亡%能量代谢
心肌缺血再灌註%生長激素%凋亡%能量代謝
심기결혈재관주%생장격소%조망%능량대사
Myocardial ischemia repeffusion%Growth hormone%Apoptosis%Energy metabolism
目的 研究生长激素预处理对大鼠心肌缺血再灌注后心肌细胞凋亡及能量代谢的影响.方法 42只大鼠采用完全随机设计方法分为3组,假手术组14只(假手术24h)、心肌缺血再灌注组14只、生长激素组14只,后2组均缺血30min,再灌注24h,其中生长激素组的每只大鼠术前皮下肌肉注射生长激素1U/kg,连续7d,其中第7次皮下注射于手术前进行.前2组每只大鼠相应皮下肌肉注射生理盐水0.5ml/d.每组中均抽取8只大鼠,以TUNEL法检测缺血区心肌细胞凋亡情况,每组中剩余的抽取5只大鼠,以高效液相色谱法测定缺血区心肌三磷酸腺苷(ATP)并进行心肌组织病理、电镜超微结构观察.结果 与假手术组比较.心肌缺血再灌注组再灌注24h后缺血区心肌细胞凋亡率和ATP消耗程度均明显上升(P＜0.05),心肌组织病理和超微结构改变明显;与心肌缺血再灌注组比较,生长激素组再灌注24h后缺血区心肌细胞凋亡率和A11P消耗程度均明显改善(P＜0.05),心肌组织病理和超微结构改变明显减轻.结论 生长激素能减少心肌缺血再灌注后心肌细胞凋亡和ATP的消耗,说明生长激素对心肌缺血再灌注后的心肌具有保护作用.
目的 研究生長激素預處理對大鼠心肌缺血再灌註後心肌細胞凋亡及能量代謝的影響.方法 42隻大鼠採用完全隨機設計方法分為3組,假手術組14隻(假手術24h)、心肌缺血再灌註組14隻、生長激素組14隻,後2組均缺血30min,再灌註24h,其中生長激素組的每隻大鼠術前皮下肌肉註射生長激素1U/kg,連續7d,其中第7次皮下註射于手術前進行.前2組每隻大鼠相應皮下肌肉註射生理鹽水0.5ml/d.每組中均抽取8隻大鼠,以TUNEL法檢測缺血區心肌細胞凋亡情況,每組中剩餘的抽取5隻大鼠,以高效液相色譜法測定缺血區心肌三燐痠腺苷(ATP)併進行心肌組織病理、電鏡超微結構觀察.結果 與假手術組比較.心肌缺血再灌註組再灌註24h後缺血區心肌細胞凋亡率和ATP消耗程度均明顯上升(P＜0.05),心肌組織病理和超微結構改變明顯;與心肌缺血再灌註組比較,生長激素組再灌註24h後缺血區心肌細胞凋亡率和A11P消耗程度均明顯改善(P＜0.05),心肌組織病理和超微結構改變明顯減輕.結論 生長激素能減少心肌缺血再灌註後心肌細胞凋亡和ATP的消耗,說明生長激素對心肌缺血再灌註後的心肌具有保護作用.
목적 연구생장격소예처리대대서심기결혈재관주후심기세포조망급능량대사적영향.방법 42지대서채용완전수궤설계방법분위3조,가수술조14지(가수술24h)、심기결혈재관주조14지、생장격소조14지,후2조균결혈30min,재관주24h,기중생장격소조적매지대서술전피하기육주사생장격소1U/kg,련속7d,기중제7차피하주사우수술전진행.전2조매지대서상응피하기육주사생리염수0.5ml/d.매조중균추취8지대서,이TUNEL법검측결혈구심기세포조망정황,매조중잉여적추취5지대서,이고효액상색보법측정결혈구심기삼린산선감(ATP)병진행심기조직병리、전경초미결구관찰.결과 여가수술조비교.심기결혈재관주조재관주24h후결혈구심기세포조망솔화ATP소모정도균명현상승(P＜0.05),심기조직병리화초미결구개변명현;여심기결혈재관주조비교,생장격소조재관주24h후결혈구심기세포조망솔화A11P소모정도균명현개선(P＜0.05),심기조직병리화초미결구개변명현감경.결론 생장격소능감소심기결혈재관주후심기세포조망화ATP적소모,설명생장격소대심기결혈재관주후적심기구유보호작용.
Objective To study the effect of growth hormone preconditioning on apoptosis and energy me tabolism in mrs with experimental myocardial ischemia reperfusion injury.Methods Forty-two rats were divided randomly into three groups:sham operated group(n=14,sham operated for 24h),IR group(n=14) and GH group(n=14).30 min ischemiafollowing by 24h reperfusion were performed in IR group and GH group.The rata in GH group were treated with subcutaneous injection of GH 0.1 U/kg body weisht per day for 7d and seventh injection was administered before the surgery.Each rat from otller groups was treated with subcutaneous injection of normal saline 0.5 ml per day.Eight rats were randomly taken from each group and terrainal deoxynueleotidyl trarm ferase mediated dUTP fluoreseein nick and labeling(TUNEL)was used to detect the changes of apoptosis.Five rats were randomly taken from each group and ATP of ischemic myocardium was measured by high-performance liquid chromatography(HPLC).Histopathology and electronic ultrastrueture of myocardial tissue were observed.Remrs Apoptosis index(AI) and depletion of ATP increased remarkably in IR group than that of sham operated group 24h after reperfusion (P＜0.05).111e rein/irkabie changes of myocardial histopathology and ultrastructure were ob served in IR group.Compared to IR group.AI and depletion of ATP in GH group were better than that of IR group 24h after reperfusion(P＜0.05),and the changes of myocardial histopathology and uhrastrueture were alleviated in GH group.Conclusion The AI and depletion of ATP were lessened due to usage of GH which indieates that GH can protect myocardium after IR.
