CAJ | 학술논문

目的建立人冠状病毒( HCoV)-NL63、HCoV-HKU1、HCoV-OC43、HCoV-229E的实时荧光定量PCR法(FQ-PCR),调查HCoV在福州地区急性呼吸道感染(ARTI)儿童中的流行情况.方法收集2006年10月-2007年4月、2007年10月-2008年4月、2008年10月-2009年4月连续3个冬春季ARTI儿童鼻咽标本共538份.其中急性上呼吸道感染(AURTI) 289例,急性下呼吸道感染(ALRTI)249例.建立分别对HCoV-NL63、HCoV-HKU1、HCoV-OC43、HCoV229E特异的FQ-PCR法.计数资料采用x2检验.结果建立了检测HCoV-NL63、HCoV-HKU1、HCoV-OC43、HCoV229E特异的FQ-PCR法,批内变异系数(CV)值与批间CV值均≤1.6％.538份标本中,共检出冠状病毒阳性41份,占7.6％.其中HCoV-NL63阳性8份,占1.5％,含AURTI 1例,ALRTI 7例；HCoV-229E阳性5份,占o.9％,含AURTI 1例,ALRTI 4例；HCoV-HKU1阳性6份,占1.1％,含AURTI 1例,ALRTI 5例；HCoV-OC43阳性22份,占4.1％,含AURTI 13例,ALRTI 9例.各型HCoV在各年份间的阳性率差异无统计学意义(P＞0.05).HCoV-OC43阳性率显著高于HCoV-NL63、HCoV-229E和HCoV-HKU1(x2=6.721,10.979,9.387;均P＜0.01).4种HCoV在连续3个冬春季节均有检出.结论 HCoV-NL63、HCoV-HKU1、HCoV-OC43和HCoV-229E可能是冬春季福州地区ARTI患儿的病毒病原,对其进行临床检测有利于ARTI的病原学诊断与流行病学研究.
목적 건립인관상병독( HCoV)-NL63、HCoV-HKU1、HCoV-OC43、HCoV-229E적실시형광정량PCR법(FQ-PCR),조사HCoV재복주지구급성호흡도감염(ARTI)인동중적류행정황.방법 수집2006년10월-2007년4월、2007년10월-2008년4월、2008년10월-2009년4월련속3개동춘계ARTI인동비인표본공538빈.기중급성상호흡도감염(AURTI) 289례,급성하호흡도감염(ALRTI)249례.건립분별대HCoV-NL63、HCoV-HKU1、HCoV-OC43、HCoV229E특이적FQ-PCR법.계수자료채용x2검험.결과 건립료검측HCoV-NL63、HCoV-HKU1、HCoV-OC43、HCoV229E특이적FQ-PCR법,비내변이계수(CV)치여비간CV치균≤1.6％.538빈표본중,공검출관상병독양성41빈,점7.6％.기중HCoV-NL63양성8빈,점1.5％,함AURTI 1례,ALRTI 7례；HCoV-229E양성5빈,점o.9％,함AURTI 1례,ALRTI 4례；HCoV-HKU1양성6빈,점1.1％,함AURTI 1례,ALRTI 5례；HCoV-OC43양성22빈,점4.1％,함AURTI 13례,ALRTI 9례.각형HCoV재각년빈간적양성솔차이무통계학의의(P＞0.05).HCoV-OC43양성솔현저고우HCoV-NL63、HCoV-229E화HCoV-HKU1(x2=6.721,10.979,9.387;균P＜0.01).4충HCoV재련속3개동춘계절균유검출.결론 HCoV-NL63、HCoV-HKU1、HCoV-OC43화HCoV-229E가능시동춘계복주지구ARTI환인적병독병원,대기진행림상검측유리우ARTI적병원학진단여류행병학연구.
Objective To establish real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) for human coronaviruses(HCoV)-NL63,HCoV-HKU1,HCoV-OC43 and HCoV-229E,and to investigate the prevalence of the four coronaviruses in children with acute respiratory tract infection(ARTI) in Fuzhou area.Methods Totally of 538 nasopharyngeal swabs were collected from pediatric patients with ARTI,including 289 specimens from children with acute upper respiratory tract infection (AURTI) and 249 from acute lower respiratory tract infection (AURTI) during three consecutive winter-spring seasons from December to April of 2006 - 2009 in Fuzhou area.All the specimens were subjected to FQ-PCR specific for HCoV-NL63,HCoV-HKU1,HCoV-OC43 and HCoV-229E,respectively.The enumeration data were analyzed by chi square test.Results The FQ-PCR methods were established for detecting HCoV-NL63,HCoV-HKU1,HCoV-OCA3 and HCoV-229E.The intraassay coefficient of variation (CV) and interassay CV were both ≤ 1.6％.The coronaviruses were detected in 41 (7.6％) children with ARTI,including HCoV-NL63 in 8 (1.5％)children (1 with AURTI,7 with ALRTI),HCoV-229E in 5 (0.9％; 1 with AURTI,4 with ALRTI),HCoV-HKU1 in 6 (1.1％; 1 with AURTI,5 with ALRTI),and HCoV-OC43 in 22(4.1％; 13 with AURTI,9 with ALRTI).The four coronaviruses were detected during each of the three winter-spring seasons and the positive rates of different periods were not significantly different (P＞0.05).The HCoV-OC43 positive rate was significantly higher than HCoV-NL63,HCoV-229E and HCoV-HKU1 (x2 =6.721,10.979,9.387; respectively; all P＜0.01).Conclusions It is suggested that the four coronaviruses might be important virus pathogens in children with ARTI in Fuzhou,China.And detection of them is needed for etiology and epidemiology evaluations for children with ARTI.