中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
4期
260-263
,共4页
杜海科%宋福成%周欣%李贺%张健鹏
杜海科%宋福成%週訢%李賀%張健鵬
두해과%송복성%주흔%리하%장건붕
博莱霉素%苦杏仁甙%光谱法,质量,基质辅助激光解吸电离
博萊黴素%苦杏仁甙%光譜法,質量,基質輔助激光解吸電離
박래매소%고행인대%광보법,질량,기질보조격광해흡전리
Bleomycin%Aamygdlin%Spectrometry,mass,matrix-assisted laser desorption-ionisation
目的 观察苦杏仁甙对博莱霉素诱导的肺纤维化大鼠血清生物标志物的影响.方法 将72只Wistar大鼠随机分为对照组、博莱霉素组和苦杏仁甙组,每组24只.分别观察7、14、28 d.博莱霉素组和苦杏仁甙组气管内一次性注入博莱霉素5 mg/kg,对照组气管内注入等体积生理盐水,同时苦杏仁甙组大鼠每天腹腔注射苦杏仁苷15 mg/kg.取肺组织制作石蜡切片,天狼猩红染色法结合偏振光显微镜观察肺组织I、Ⅲ型胶原的表达.表面增强激光解吸附离子化飞行时间质谱(SELDI-TOF MS)结合弱阳离子蛋白芯片检测各组大鼠混合血清差异蛋白的表达.结果 肺组织石蜡切片天狼猩红染色显示肺纤维化动物模犁肺组织存在持续纤维化反应.模型建立7、14、28 d后,3组均检测到4种差异表达的蛋白,其质荷比分别为3530.7,7043.5,8332.6和9068.0.与对照组比较,博莱霉素模型组7和28 d时质荷比为3530.7的血清蛋白标志物峰强度比值在>2,在7、14、28 d时质荷比为7043.5,8332.6和9068.0的血清蛋白标志物峰强度比值均>2,有明显差异.与博莱霉素组比较,苦杏仁甙组质荷比为7043.5(7 d),质荷比为8332.6(28 d)和9068.0(14 d)的血清蛋白标志物峰强度比值均>2,有明显差异.结论 苦杏仁甙可抑制博莱霉素诱导的大鼠血清蛋白标志物峰强度的增加.
目的 觀察苦杏仁甙對博萊黴素誘導的肺纖維化大鼠血清生物標誌物的影響.方法 將72隻Wistar大鼠隨機分為對照組、博萊黴素組和苦杏仁甙組,每組24隻.分彆觀察7、14、28 d.博萊黴素組和苦杏仁甙組氣管內一次性註入博萊黴素5 mg/kg,對照組氣管內註入等體積生理鹽水,同時苦杏仁甙組大鼠每天腹腔註射苦杏仁苷15 mg/kg.取肺組織製作石蠟切片,天狼猩紅染色法結閤偏振光顯微鏡觀察肺組織I、Ⅲ型膠原的錶達.錶麵增彊激光解吸附離子化飛行時間質譜(SELDI-TOF MS)結閤弱暘離子蛋白芯片檢測各組大鼠混閤血清差異蛋白的錶達.結果 肺組織石蠟切片天狼猩紅染色顯示肺纖維化動物模犛肺組織存在持續纖維化反應.模型建立7、14、28 d後,3組均檢測到4種差異錶達的蛋白,其質荷比分彆為3530.7,7043.5,8332.6和9068.0.與對照組比較,博萊黴素模型組7和28 d時質荷比為3530.7的血清蛋白標誌物峰彊度比值在>2,在7、14、28 d時質荷比為7043.5,8332.6和9068.0的血清蛋白標誌物峰彊度比值均>2,有明顯差異.與博萊黴素組比較,苦杏仁甙組質荷比為7043.5(7 d),質荷比為8332.6(28 d)和9068.0(14 d)的血清蛋白標誌物峰彊度比值均>2,有明顯差異.結論 苦杏仁甙可抑製博萊黴素誘導的大鼠血清蛋白標誌物峰彊度的增加.
목적 관찰고행인대대박래매소유도적폐섬유화대서혈청생물표지물적영향.방법 장72지Wistar대서수궤분위대조조、박래매소조화고행인대조,매조24지.분별관찰7、14、28 d.박래매소조화고행인대조기관내일차성주입박래매소5 mg/kg,대조조기관내주입등체적생리염수,동시고행인대조대서매천복강주사고행인감15 mg/kg.취폐조직제작석사절편,천랑성홍염색법결합편진광현미경관찰폐조직I、Ⅲ형효원적표체.표면증강격광해흡부리자화비행시간질보(SELDI-TOF MS)결합약양리자단백심편검측각조대서혼합혈청차이단백적표체.결과 폐조직석사절편천랑성홍염색현시폐섬유화동물모리폐조직존재지속섬유화반응.모형건립7、14、28 d후,3조균검측도4충차이표체적단백,기질하비분별위3530.7,7043.5,8332.6화9068.0.여대조조비교,박래매소모형조7화28 d시질하비위3530.7적혈청단백표지물봉강도비치재>2,재7、14、28 d시질하비위7043.5,8332.6화9068.0적혈청단백표지물봉강도비치균>2,유명현차이.여박래매소조비교,고행인대조질하비위7043.5(7 d),질하비위8332.6(28 d)화9068.0(14 d)적혈청단백표지물봉강도비치균>2,유명현차이.결론 고행인대가억제박래매소유도적대서혈청단백표지물봉강도적증가.
Objective To evaluate effect of amygdlin on expression of four biomarkers in the animal model of pulmonary fibrosis induced by bleomycin.Methods Rats were given one dose (5 mg/kg)of bleomyem in bleomycin-treated groups，amygdlin-treated groups and saline in controls by intratrachealinstil.lation exposed surglcally,The amygdlin-treated groups rats were treated with intraperitoneal injeetion of amygdlin(15 mg·kg~(-1)·day~(-1)).The rats were sacrificed 7，14 and 28 days after bleomycin administration.Polarized light microscopy and Image-Pro Plus detected I and Ⅲcollagen expressed in Paraffin-embedded lung sections stained with Sirius red.Surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS)with weak cationic proteinchip(CMiO)detected differentially expressed proteins in the pooled serum samples of all groups.Results Consistent fibrotic responses were found in all bleomycin and amygdlin-tread groups.On the 7th，14th and 28th day after bleomycin or saline instillation.four differentially expressed proteins were detected in the pooled serum of all groups rats，consisting of 4 proteins with mass/charge ratio of 3530.7，7043.5，8332.6 and 9068.0，respectively.Compared with control groups，pmtein peaks intensity ratio with mass/charge ratio of 3530.7 on 7，28 d and 7043.5，8332.6 and 9068.0 on 7.14 and 28 d was>2 in bleomycin-treated groups.Compared with amygdlin-treated groups，protein peaks intensitv with mass/charge ratio of 3530.7 at 7，14，28 d had no change almost，but protein peaks intensitv ratio with mass/charge ratio of 7043.5 at 7 d，8332.6 on 28 d and 9068.0 on 14 d was>2 in bleomycin-tread grouDs.All the four protein peaks intensity had no change almost at other point.Conclusion Amygdlin may reduce the bleom-ycin-induced increase of differentially expressed protein peak intensities in rat serum.
