CAJ | 학술논문

目的通过检测溃疡性结肠炎(UC)患者外周血中IL-22及相关CD4+T细胞亚群的表达,探讨其在UC发病机制中的可能作用.方法以我院住院治疗的35例UC患者及35例健康对照者为研究对象,应用ELISA法检测外周血血浆中IL-22的含量,采用流式细胞术检测Th1、Th17、Th22细胞亚群的比例,并分析其与疾病活动度的关系.结果 UC组患者血浆中IL-22的含量为(354.12±104.22) pg/ml,显著高于对照组(P＜0.05),其中重度患者增高明显；UC组患者外周血Th17细胞比例[(2.36±0.94)％]显著高于对照组(P＜0.05),其中,中、重度患者增高明显；UC组患者外周血Th22细胞比例[(2.27±0.87)％]显著高于对照组(P＜0.05),其中,重度患者增高明显；而UC组患者Th1细胞比例与对照组比较尤明显差异.结论 UC患者外周血中IL-22水平、Th17、Th22细胞比例明显升高,且与疾病活动度密切相关.
목적 통과검측궤양성결장염(UC)환자외주혈중IL-22급상관CD4+T세포아군적표체,탐토기재UC발병궤제중적가능작용.방법 이아원주원치료적35례UC환자급35례건강대조자위연구대상,응용ELISA법검측외주혈혈장중IL-22적함량,채용류식세포술검측Th1、Th17、Th22세포아군적비례,병분석기여질병활동도적관계.결과 UC조환자혈장중IL-22적함량위(354.12±104.22) pg/ml,현저고우대조조(P＜0.05),기중중도환자증고명현；UC조환자외주혈Th17세포비례[(2.36±0.94)％]현저고우대조조(P＜0.05),기중,중、중도환자증고명현；UC조환자외주혈Th22세포비례[(2.27±0.87)％]현저고우대조조(P＜0.05),기중,중도환자증고명현；이UC조환자Th1세포비례여대조조비교우명현차이.결론 UC환자외주혈중IL-22수평、Th17、Th22세포비례명현승고,차여질병활동도밀절상관.
Objective To detect the expression of interlenkin-22 (IL-22) and relative CD4+ T cell subsets in patients with ulcerative colitis (UC),and to explore their roles in the pathogenesis of UC.Methods Thirty-five adult UC patients were enrolled in this study and 35 healthy subjects were taken as control.Plasma IL-22 level was quantified by ELISA.The percentages of Th1,Th17 and Th22 cells in peripheral blood were determined by flow cytometry.The relationships of these results and disease activity were analyzed.Results Plasma IL-22 levels in UC patients [ (354.12±104.22 ) pg/ml ]were significantly higher than that of healthy controls ( P＜0.05 ),and the levels increased significantly in severely active patients.The percentages of Th17 cells in UC patients [ (2.36±0.94) ％ ]were elevated compared to healthy controls ( P＜0.05 ),and the percentages increased significantly in moderately active and severely active patients.The percentages of Th22 cells in UC patients [ (2.27±0.87 ) ％ ]were elevated compared to healthy controls (P±0.05),and the percentages increased significantly in severely active patients.The percentage of Th1 cells was not significantly different between UC patients and normal controls.Conclusion Our resuits demonstrate elevated IL-22 correlated to Th17 and Th22 cells may play an important role in the immunopathogenesis of UC.