中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2011年
6期
437-442
,共6页
邵家松%孟德峰%岳毅刚%周海%花鸣春%张敏
邵傢鬆%孟德峰%嶽毅剛%週海%花鳴春%張敏
소가송%맹덕봉%악의강%주해%화명춘%장민
环磷酰胺%口服%瘢痕,肥大性
環燐酰胺%口服%瘢痕,肥大性
배린선알%구복%반흔,비대성
Cyclophosphamide%Feeding%Cicatrix,hypertrophic
目的 通过饲服环磷酰胺观察其对兔耳早期瘢痕的影响,以探讨临床应用口服免疫抑制剂防治早期瘢痕的可行性.方法 以32只新西兰白兔兔耳建立增生性瘢痕动物模型,并将其随机分成4组,即蒸馏水组(A组)、5mg·kg-1·d-1环磷酰胺组(B组)、10 mg·kg-1·d-1组(C组)及30mg·kg-1·d-1(D组).在制作模型前及灌胃后14、28 d时分别采血观察白细胞、淋巴细胞变化.28 d时取组织HE染色、VG染色法观察瘢痕增生指数(HI)、成纤维细胞密度(NA)、胶原纤维面密度(AA)数值变化.各组数据(HI、NA、AA)比较采用方差分析,方差不齐时作秩和检验.结果 14 d时,A、B、C、D组白细胞数量分别为(8.62 +0.58)×109/L、(4.48±0.41)×109/L、(2.7±0.26)×109/L、(1.33±0.27)×109/L,淋巴细胞数量分别为(3.11±0.21)×109/L、(1.67 +0.16)×109/L、(0.42 +0.10)×109/L、(0.40 +0.09)×109/L;28 d时,A、B、C、D组白细胞数量分别为(8.63±0.53)× 109/L、(5.10±0.27)×109/L、(3.10±0.26)× 109/L、( 1.98±0.20)×109/L,淋巴细胞数量分别为(3.06±0.16)×109/L、( 2.08±0.14)×109/L、(0.96±0.19)×109/L、(0.14±0.07)×109/L,实验各剂量组(B、C、D组)白细胞数量和淋巴细胞数量下降,且随药物剂量的增加而下降更明显,呈负相关,各组间比较差异有统计学意义(P＜0.05).A、B、C、D组HI值分别为3.02±0.24、2.59±0.43、2.06±0.19、1.63±0.11,AA值分别为40.49±2.07、35.29±1.99、28.36±1.87、24.99±1.82,NA值分别为4570.5±259.3、4222.5±199.6、3540.3±170.3、3341.4±228.8,对照组与实验各剂量组的HI、AA、NA数值之间比较差异有统计学意义(P＜0.01),且随剂量的增加,除NA值C和D组比较差异无统计学意义外,其余实验各剂量组HI、AA、NA值变小更明显,呈负相关,各组之间比较差异有统计学意义(P＜0.05).结论 饲服环磷酰胺在抑制白细胞和淋巴细胞的同时能明显抑制瘢痕增生,对兔耳早期增生性瘢痕有明显的防治作用.
目的 通過飼服環燐酰胺觀察其對兔耳早期瘢痕的影響,以探討臨床應用口服免疫抑製劑防治早期瘢痕的可行性.方法 以32隻新西蘭白兔兔耳建立增生性瘢痕動物模型,併將其隨機分成4組,即蒸餾水組(A組)、5mg·kg-1·d-1環燐酰胺組(B組)、10 mg·kg-1·d-1組(C組)及30mg·kg-1·d-1(D組).在製作模型前及灌胃後14、28 d時分彆採血觀察白細胞、淋巴細胞變化.28 d時取組織HE染色、VG染色法觀察瘢痕增生指數(HI)、成纖維細胞密度(NA)、膠原纖維麵密度(AA)數值變化.各組數據(HI、NA、AA)比較採用方差分析,方差不齊時作秩和檢驗.結果 14 d時,A、B、C、D組白細胞數量分彆為(8.62 +0.58)×109/L、(4.48±0.41)×109/L、(2.7±0.26)×109/L、(1.33±0.27)×109/L,淋巴細胞數量分彆為(3.11±0.21)×109/L、(1.67 +0.16)×109/L、(0.42 +0.10)×109/L、(0.40 +0.09)×109/L;28 d時,A、B、C、D組白細胞數量分彆為(8.63±0.53)× 109/L、(5.10±0.27)×109/L、(3.10±0.26)× 109/L、( 1.98±0.20)×109/L,淋巴細胞數量分彆為(3.06±0.16)×109/L、( 2.08±0.14)×109/L、(0.96±0.19)×109/L、(0.14±0.07)×109/L,實驗各劑量組(B、C、D組)白細胞數量和淋巴細胞數量下降,且隨藥物劑量的增加而下降更明顯,呈負相關,各組間比較差異有統計學意義(P＜0.05).A、B、C、D組HI值分彆為3.02±0.24、2.59±0.43、2.06±0.19、1.63±0.11,AA值分彆為40.49±2.07、35.29±1.99、28.36±1.87、24.99±1.82,NA值分彆為4570.5±259.3、4222.5±199.6、3540.3±170.3、3341.4±228.8,對照組與實驗各劑量組的HI、AA、NA數值之間比較差異有統計學意義(P＜0.01),且隨劑量的增加,除NA值C和D組比較差異無統計學意義外,其餘實驗各劑量組HI、AA、NA值變小更明顯,呈負相關,各組之間比較差異有統計學意義(P＜0.05).結論 飼服環燐酰胺在抑製白細胞和淋巴細胞的同時能明顯抑製瘢痕增生,對兔耳早期增生性瘢痕有明顯的防治作用.
목적 통과사복배린선알관찰기대토이조기반흔적영향,이탐토림상응용구복면역억제제방치조기반흔적가행성.방법 이32지신서란백토토이건립증생성반흔동물모형,병장기수궤분성4조,즉증류수조(A조)、5mg·kg-1·d-1배린선알조(B조)、10 mg·kg-1·d-1조(C조)급30mg·kg-1·d-1(D조).재제작모형전급관위후14、28 d시분별채혈관찰백세포、림파세포변화.28 d시취조직HE염색、VG염색법관찰반흔증생지수(HI)、성섬유세포밀도(NA)、효원섬유면밀도(AA)수치변화.각조수거(HI、NA、AA)비교채용방차분석,방차불제시작질화검험.결과 14 d시,A、B、C、D조백세포수량분별위(8.62 +0.58)×109/L、(4.48±0.41)×109/L、(2.7±0.26)×109/L、(1.33±0.27)×109/L,림파세포수량분별위(3.11±0.21)×109/L、(1.67 +0.16)×109/L、(0.42 +0.10)×109/L、(0.40 +0.09)×109/L;28 d시,A、B、C、D조백세포수량분별위(8.63±0.53)× 109/L、(5.10±0.27)×109/L、(3.10±0.26)× 109/L、( 1.98±0.20)×109/L,림파세포수량분별위(3.06±0.16)×109/L、( 2.08±0.14)×109/L、(0.96±0.19)×109/L、(0.14±0.07)×109/L,실험각제량조(B、C、D조)백세포수량화림파세포수량하강,차수약물제량적증가이하강경명현,정부상관,각조간비교차이유통계학의의(P＜0.05).A、B、C、D조HI치분별위3.02±0.24、2.59±0.43、2.06±0.19、1.63±0.11,AA치분별위40.49±2.07、35.29±1.99、28.36±1.87、24.99±1.82,NA치분별위4570.5±259.3、4222.5±199.6、3540.3±170.3、3341.4±228.8,대조조여실험각제량조적HI、AA、NA수치지간비교차이유통계학의의(P＜0.01),차수제량적증가,제NA치C화D조비교차이무통계학의의외,기여실험각제량조HI、AA、NA치변소경명현,정부상관,각조지간비교차이유통계학의의(P＜0.05).결론 사복배린선알재억제백세포화림파세포적동시능명현억제반흔증생,대토이조기증생성반흔유명현적방치작용.
Objective To investigate the feasibility of prevention and treatment of early scar through observing the effect of feeding immunosuppressive drug cyclophosphamide on rabbit ears hypertrophic scar tissue in early stage.Methods Thirty-two Rabbit ears were used to establish animal models for hypertrophic scar and randomly divided into four groups:group of distilled water( A )、group of cyclophosphamide 5 mg·kg-1 ·d-1 ( B)、group of 10 mg·kg-1 ·d -1 ( C)、group of 30 mg·kg-1 ·d -1 (D).Before animal models were built and after administration for 14 days,28 days,leukocytes and lymphocytes were detected.After 28 days,specimens were harvested and underwent HE staining and VG staining in order to assess HI,NA,AA value changes.The data (HI,NA,AA) from each group were compared by analysis of variance,and the variance for the rank sum test when missing.Results On the 14th day,the number of leukocytes in group A,B,C,D were (8.62 ±0.58) × 109/L,(4.48 ±0.41) × 109/L,(2.7±0.26) × 109/L,( 1.33 ± 0.27 ) × 109/L; the number of lymphocytes in group A,B,C,D were ( 3.11 ±0.21) ×109/L,(1.67 ±0.16) × 109/L,(0.42 ±0.10) × 109/L,(0.40 ±0.09) × 109/L.On the 28th day,the number of leukocytes in group A,B,C,D was (8.63 +0.53) × 109/L,(5.10 ±0.27) × 109/L,(3.10 ± 0.26) × 109/L,( 1.98 ± 0.20) × 109/L; the number of lymphocytes A,B,C,D was ( 3.06 ±0.16) × 109/L,(2.08 ±0.14) × 109/L,(0.96 ±0.19) × 109/L,(0.14 ±0.07) × 109/L.On the 14th day and 28th day,the number of leukocytes and lymphocytes in experimental groups was reduced,showing a negative relation with cyclophosphamide dose ( P ＜ 0.05 ).The HI in group of A,B,C,D was 3.02 ±0.24,2.59 ± 0.43,2.06 ± 0.19,1.63 ± 0.11 ; the AA was 40.49 ± 2.07,35.29 ± 1.99,28.36 ± 1.87,24.99 ± 1.82; the NA was 4570.5 ± 259.3,4222.5 ± 199.6,3540.3 ± 170.3,3341.4 ± 228.8.The difference in HI,AA,NA between control group and any of the experimental groups was statistically significant (P ＜0.01 ).Each group,with the dose increased,except NA content of group C and D,the HI,AA,NA was more smaller,negative correlation,the difference was statistically significant(P ＜0.05).Conclusions Feeding cyclophosphamide can inhibit leukocytes and lymphocytes number,so as to inhibit the proliferative activity of hypertrophic scar.It has significant effect on prevention of hypertrophic scar on rabbit ears in early stage.
