CAJ | 학술논문

[目的] 从自然界中筛选高效耐冷苯酚降解菌, 并且研究其降解特性.[方法]采用富集培养法,用液相色谱法和分光光度法分析菌株降解苯酚的性能.[结果] 从常州城北污水处理厂的污水曝气池中,分离6到1株能以苯酚为唯一碳源生长的耐冷细菌Phe311菌株.经16 S rDNA基因序列分析,该菌被鉴定为假单胞菌.Phe311在6 ℃ 96 h内对300 mg/L 苯酚降解率达96.4%.Phe311降解苯酚的最适条件为pH值7.0,30 ℃,接种量1%.[结论] 菌株Phe311在最适条件下72 h内可以完全降解苯酚.
[목적] 종자연계중사선고효내랭분분강해균, 병차연구기강해특성.[방법]채용부집배양법,용액상색보법화분광광도법분석균주강해분분적성능.[결과] 종상주성북오수처리엄적오수폭기지중,분리6도1주능이분분위유일탄원생장적내랭세균Phe311균주.경16 S rDNA기인서렬분석,해균피감정위가단포균.Phe311재6 ℃ 96 h내대300 mg/L 분분강해솔체96.4%.Phe311강해분분적최괄조건위pH치7.0,30 ℃,접충량1%.[결론] 균주Phe311재최괄조건하72 h내가이완전강해분분.
[Objective] The research aimed to isolate the cold resistant bacteria, which could degrade phenol.Its degradative ability was studied.[Method] Enrichment method was adopted.HPLC and spectrophotometer were used to study the capability.[Result] From waste water of Changzhou wastewater treatment plant, a strain Phe311 was isolated, which could degrade phenol and use phenol as the sole carbon source.Through 16 S rDNA genetical analysis, Phe311 was identified as Pseudomonas Migula.The degradative rate for phenol at 300 mg/L was 96.4% in 96 hours at 6 ℃.The optimum condition was as follows: 30 ℃, pH value 7.0, with inoculating quantity at 1%.[Conclusion] The strain Phe311 could degrade phenol completely in 72 hours.