兰州大学学报（医学版）
蘭州大學學報（醫學版）
란주대학학보（의학판）
JOURNAL OF LANZHOU UNIVERSITY(MEDICAL SCIENCES)
2009年
2期
51-53
,共3页
曹芳%李耀曾%杜兴%夏华
曹芳%李耀曾%杜興%夏華
조방%리요증%두흥%하화
二次膜分离%藏牦牛血%凝血酶%凝血酶原
二次膜分離%藏牦牛血%凝血酶%凝血酶原
이차막분리%장모우혈%응혈매%응혈매원
double-membrane separation%blood from Tibet yak%thrombin%thrombinogen
目的 探讨二次膜分离提取藏牦牛血凝血酶的工艺.方法 根据藏牦牛血凝血酶原及其凝血酶的分子量,进行二次膜分离技术提取藏牦牛血凝血酶.结果 采用二次膜分离技术提取藏牦牛血凝血酶.其比活力与活力回收分别比有机溶剂沉淀法提高了76.72 U/mg蛋白质和35.0%;其比活力比一次膜分离法提高了41.14 U/mg蛋白质.结论 工艺可靠,方法可行,适宜于中小企业的生产.
目的 探討二次膜分離提取藏牦牛血凝血酶的工藝.方法 根據藏牦牛血凝血酶原及其凝血酶的分子量,進行二次膜分離技術提取藏牦牛血凝血酶.結果 採用二次膜分離技術提取藏牦牛血凝血酶.其比活力與活力迴收分彆比有機溶劑沉澱法提高瞭76.72 U/mg蛋白質和35.0%;其比活力比一次膜分離法提高瞭41.14 U/mg蛋白質.結論 工藝可靠,方法可行,適宜于中小企業的生產.
목적 탐토이차막분리제취장모우혈응혈매적공예.방법 근거장모우혈응혈매원급기응혈매적분자량,진행이차막분리기술제취장모우혈응혈매.결과 채용이차막분리기술제취장모우혈응혈매.기비활력여활력회수분별비유궤용제침정법제고료76.72 U/mg단백질화35.0%;기비활력비일차막분리법제고료41.14 U/mg단백질.결론 공예가고,방법가행,괄의우중소기업적생산.
Objective To establish crafts to extract thrombin from the blood of the Tibet yak by double-membrane separation. Methods According to molecular weights of blood thrombino- gen and thrombin of Tibetan yak,thrombin from the blood of the Tibet yak was extracted by double-membrane separation. Results The specific activity and activity recollection of thrombin were improved by 76.72 U/mgpro and 35.0% respectively when compared with organic solvent precipitation method,and the specific activity of thrombin was improved by 41.14 U/mg pro when compared with single membrane separation. Conclusion The craft is credible and feasible,the and can be used for the production of small and medium-sized scales.
