CAJ | 학술논문

观察蛋白酪氨酸激酶抑制剂Genistein对人肺腺癌细胞株A549细胞侵袭能力的影响,探讨Genistein抑制肺癌细胞侵袭的可能机制.以不同浓度Genistein(20 μmol/L和40 μmol/L)作用于A549细胞3 d 后,分别用基质胶侵袭模型、黏附基质分析、Transwell小室趋化运动模型、细胞骨架蛋白染色及RT-PCR法来研究药物处理后细胞侵袭、黏附、运动、聚合型骨架蛋白(F-actin)以及基质金属蛋白酶基因表达的改变.经Genistein处理后,A549细胞的F-actin聚合减少,侵袭能力明显下降,趋化运动能力降低,基质金属蛋白酶抑制剂(TIMP-1)基因相对表达量增加,但黏附率没有降低.Genistein可降低肺癌细胞的迁移、侵袭能力.F-actin聚合减少,TIMP-1的相对表达量增加,可能是Genistein抑制肺癌细胞侵袭的机制之一.
관찰단백락안산격매억제제Genistein대인폐선암세포주A549세포침습능력적영향,탐토Genistein억제폐암세포침습적가능궤제.이불동농도Genistein(20 μmol/L화40 μmol/L)작용우A549세포3 d 후,분별용기질효침습모형、점부기질분석、Transwell소실추화운동모형、세포골가단백염색급RT-PCR법래연구약물처리후세포침습、점부、운동、취합형골가단백(F-actin)이급기질금속단백매기인표체적개변.경Genistein처리후,A549세포적F-actin취합감소,침습능력명현하강,추화운동능력강저,기질금속단백매억제제(TIMP-1)기인상대표체량증가,단점부솔몰유강저.Genistein가강저폐암세포적천이、침습능력.F-actin취합감소,TIMP-1적상대표체량증가,가능시Genistein억제폐암세포침습적궤제지일.
The effects of tyrosine protein kinase inhibitor, Genistein, on invasion ability of human lung adenocarcinoma cell line A549 were observed and its possible mechanism was investigated. Three days after A549 cells were reacted with different concentration Genistein (20 μmol/L and 40 μmol/L) the changes of invasion, adhesion, movement and migration, as well as expression of F-actin as well as matrix metalloproteinase genes were studied by matrigel invasion models, adhesion matrix analysis, Transwell chamber chemotactic movement models, cytoskeleton protein staining and RT-PCR respectively after the medicine treatment. The results showed that after treated with Genistein, polymerization of F-actin of A549 cells declined, invasive and moving abilities were significantly reduced, chemotactic movement abilities declined, relative expression of matrix metalloproteinase inhibitor (TIMP-1) increased, however, no reduction of adhesion ratio was found. Therefore, Genistein could decrease the lung cancer cell to migrate and the ability of invasion. The reduction of F-actin polymerization and the increment of relative expression of TIMP-1 might be one of the mechanisms of Genistein to inhibit lung cancer cells to invade.