CAJ | 학술논문

目的探讨姜黄素对人增生性瘢痕(hyperplastic scar)成纤维细胞(fibroblast,FB)增殖及胶原合成的影响,以寻找治疗人增生性瘢痕的有效药物.方法应用组织块法分离培养瘢痕和正常真皮组织的FB,在倒置相差显微镜下观察细胞形态,应用细胞计数方法绘制细胞生长曲线,测定细胞生长速度;采用不同浓度的姜黄素(0、12.5、25、50和100 μmol/L)刺激瘢痕FB,采用四氮甲基唑盐比色法(MTT法)检测姜黄素对FB生长的抑制效应,采用RT-PCR检测姜黄素对FBcxl(Ⅰ)前胶原基因的转录活性的影响.结果细胞生长曲线显示:增生性瘢痕FB倍增时间约为5天,正常皮肤FB后细胞倍增时间为4天,两者比较,差异有统计学意义(P<0.05);MTT检测结果显示:12.5 μmol/L姜黄素作用于增生性瘢痕FB,表现为增殖倾向,其光吸收值(A值)显著地高于正常对照组.而在25～100 μmol/L的浓度范围内,随着姜黄素药物浓度的增加,其抑制作用逐渐增强,50 μmol/L和100 μmol/L姜黄素能显著抑制瘢痕FB增殖(P<0.05);此外,50 μmol/L和100 μmol/L姜黄素能够显著抑制瘢痕FB Ⅰ型胶原合成(P<0.01).结论较高浓度的姜黄素能有效抑制增生性瘢痕FB增殖和Ⅰ型胶原合成,对增生性瘢痕可能具有治疗作用.
목적 탐토강황소대인증생성반흔(hyperplastic scar)성섬유세포(fibroblast,FB)증식급효원합성적영향,이심조치료인증생성반흔적유효약물.방법 응용조직괴법분리배양반흔화정상진피조직적FB,재도치상차현미경하관찰세포형태,응용세포계수방법회제세포생장곡선,측정세포생장속도;채용불동농도적강황소(0、12.5、25、50화100 μmol/L)자격반흔FB,채용사담갑기서염비색법(MTT법)검측강황소대FB생장적억제효응,채용RT-PCR검측강황소대FBcxl(Ⅰ)전효원기인적전록활성적영향.결과 세포생장곡선현시:증생성반흔FB배증시간약위5천,정상피부FB후세포배증시간위4천,량자비교,차이유통계학의의(P<0.05);MTT검측결과현시:12.5 μmol/L강황소작용우증생성반흔FB,표현위증식경향,기광흡수치(A치)현저지고우정상대조조.이재25～100 μmol/L적농도범위내,수착강황소약물농도적증가,기억제작용축점증강,50 μmol/L화100 μmol/L강황소능현저억제반흔FB증식(P<0.05);차외,50 μmol/L화100 μmol/L강황소능구현저억제반흔FB Ⅰ형효원합성(P<0.01).결론 교고농도적강황소능유효억제증생성반흔FB증식화Ⅰ형효원합성,대증생성반흔가능구유치료작용.
Objective To seek an effective drug for treatment of human hyperplastic scar through studying the effects of curcumin on fibroblast growth and collagen synthesis. Methods Fibroblasts derived from scar tissue and from normal epidermal tissue were isolated and cultured separately with tissue-block method, their morphology were observed under invert phase contrast microscope, their growth curve was drawn respectively to determine the speed of growth. Then, fibroblasts from scar were stimulated with curcumin in different concentrations (0, 12.5, 25, 50 and 100 μmol/L) for detecting the inhibitory effect of curcumin on growth of fibroblasts using MTT methods and that on activity of precollagen α-1 gene transcription in fibroblast was detected by RTPCR. Results The cell growth curve showed that double-multiplying time was 5 days in fibroblasts from scar and 4 days in those from normal dermis, showing significant difference between them (P<0.05). MTT showed that curcumin in 12.5 μmol/L showed a cell proliferation enhancing trend, and its absorbance value was significantly higher than that in the normal group, but the effect turned to inhibition when concentration increased to over 25-100 μmol/L, and became significant inhibition at concentration of 50 and 100 μmol/L. Besides, curcumin also showed markedly inhibition on collagen type Ⅰ synthesis in fibroblasts (P<0.01). Conclusion High concentration curcumin can inhibit effectively the fibroblast proliferation and collagen Ⅰ synthesis in hyperplastic scar, therefore, may has therapeutic effect on the disease in human being.