浙江大学学报（医学版）
浙江大學學報（醫學版）
절강대학학보（의학판）
JOURNAL OF ZHEJIANG UNIVERSITY MEDICAL SCIENCES
2010年
1期
11-16
,共6页
核胞浆转运蛋白类%氧化性应激%基因表达%反应元件%有机铂化合物%抗药性%肿瘤%谷胱甘肽%肺肿瘤
覈胞漿轉運蛋白類%氧化性應激%基因錶達%反應元件%有機鉑化閤物%抗藥性%腫瘤%穀胱甘肽%肺腫瘤
핵포장전운단백류%양화성응격%기인표체%반응원건%유궤박화합물%항약성%종류%곡광감태%폐종류
Karyopherins%Oxidative stress%Gene expression%Response elements%Organoplatinum compounds%Drug resistance,neoplasm%Glutathione%Lung neoplasms
目的:建立Nrf2低表达的H460细胞株,可增敏奥沙利铂对肿瘤细胞的抗增殖作用,同时为Nrf2-ARE信号通路的研究提供便捷途径.方法:H460细胞转染pRS-hNrf2后经过筛选得到多个单克隆细胞株,通过Western blotting和GSH含量的检测方法分析各细胞株中Nrf2及其调控基因的表达以及GSH含量的变化,用MTS法检测细胞株对抗癌药物的敏感性.结果:H460细胞转染pRS-hNrf2后筛选建立Nrf2低表达的稳定细胞株H460-C9和H460-C13,与对照组相比其GSH含量均有显著降低(P_(C9)= 0.00249,P_(C13)= 0.03944);同时,MTS检测表明,抗癌药物奥沙利铂和阿霉素的抗细胞增殖作用在H460-C9和H460-C13细胞株中明显增强.结论:成功建立Nrf2低表达的H460-C9和H460-C13细胞株,与对照组细胞株相比,低表达Nrf2可增敏奥沙利铂对肿瘤细胞的抗增殖作用.
目的:建立Nrf2低錶達的H460細胞株,可增敏奧沙利鉑對腫瘤細胞的抗增殖作用,同時為Nrf2-ARE信號通路的研究提供便捷途徑.方法:H460細胞轉染pRS-hNrf2後經過篩選得到多箇單剋隆細胞株,通過Western blotting和GSH含量的檢測方法分析各細胞株中Nrf2及其調控基因的錶達以及GSH含量的變化,用MTS法檢測細胞株對抗癌藥物的敏感性.結果:H460細胞轉染pRS-hNrf2後篩選建立Nrf2低錶達的穩定細胞株H460-C9和H460-C13,與對照組相比其GSH含量均有顯著降低(P_(C9)= 0.00249,P_(C13)= 0.03944);同時,MTS檢測錶明,抗癌藥物奧沙利鉑和阿黴素的抗細胞增殖作用在H460-C9和H460-C13細胞株中明顯增彊.結論:成功建立Nrf2低錶達的H460-C9和H460-C13細胞株,與對照組細胞株相比,低錶達Nrf2可增敏奧沙利鉑對腫瘤細胞的抗增殖作用.
목적:건립Nrf2저표체적H460세포주,가증민오사리박대종류세포적항증식작용,동시위Nrf2-ARE신호통로적연구제공편첩도경.방법:H460세포전염pRS-hNrf2후경과사선득도다개단극륭세포주,통과Western blotting화GSH함량적검측방법분석각세포주중Nrf2급기조공기인적표체이급GSH함량적변화,용MTS법검측세포주대항암약물적민감성.결과:H460세포전염pRS-hNrf2후사선건립Nrf2저표체적은정세포주H460-C9화H460-C13,여대조조상비기GSH함량균유현저강저(P_(C9)= 0.00249,P_(C13)= 0.03944);동시,MTS검측표명,항암약물오사리박화아매소적항세포증식작용재H460-C9화H460-C13세포주중명현증강.결론:성공건립Nrf2저표체적H460-C9화H460-C13세포주,여대조조세포주상비,저표체Nrf2가증민오사리박대종류세포적항증식작용.
Objective: To establish a stable H460 cell line with Nrf2 down-regulation to study the role of Nrf2 in Oxaliplatin resistance.Methods: Nrf2 down-regulated H460 cell line was obtained by transfecting cells with pRS-hNrf2 and followed by screening for Nrf2 expression by Western blotting.The cell lines were further characterized by analysing cellular GSH levels and cytotoxicity to anti-cancer drugs with MTS.Results: Nrf2 down-regulated H460 cell lines were established successfully. Cellular GSH level reduced significantly in the H460-C9 and H460-C13 compared to control cells(P_(C9)= 0.00249,P_(C13)= 0.03944). Down-regulation of Nrf2 in H460 sensitized the anti-cancer effect of Oxaliplatin and Doxorubicin.Conclusion: Nrf2 plays an important role in drug resistance.Down regulation of Nrf2 in H460 cell enhances cytotoxicity of Oxaliplatin.
