中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
12期
1469-1471
,共3页
沈婵%饶艳%王焱林%张宗泽%王成夭%李卉%王赟
瀋嬋%饒豔%王焱林%張宗澤%王成夭%李卉%王赟
침선%요염%왕염림%장종택%왕성요%리훼%왕빈
异氟醚%心肌再灌注损伤%NF-κB
異氟醚%心肌再灌註損傷%NF-κB
이불미%심기재관주손상%NF-κB
Isoflurane%Myocardial reperfusion injury%NF-kappa B
目的 探讨乳化异氟醚预处理对大鼠心肌缺血再灌注时NF-κB活性的影响.方法 健康雄性SD大鼠48只,体重230～280 g,随机分为4组(n=12):假手术组(S组)仅穿线不结扎;缺血再灌注组(IR组)结扎左冠状动脉前降支30 min,恢复灌注120 min制备心肌缺血再灌注损伤模型;脂肪乳剂组(L组)经股静脉输注30%脂肪乳剂4 ml·kg-1·h-1 30 min后制备模型;乳化异氟醚组(EI组)经股静脉输注乳化异氟醚4 ml·kg-1·h-130 min,洗脱15 min后制备模型.于再灌注120 min时采集股动脉血样,采用ELISA法测定血清心肌肌钙蛋白Ⅰ(cTnI)、IL-6浓度及磷酸肌酸激酶同工酶(CK-MB)活性,随后处死大鼠取心脏,采用Western blot法测定心肌细胞NF-κB活性,电镜下观察心肌细胞的超微结构.结果 与S组比较,IR组、EI组和L组心肌细胞NF-κB活性升高,血清IL-6、cTnI浓度及CK-MB活性升高(P＜0.05或0.01);与IR组比较,ET组心肌细胞NF-κB活性降低,血清IL-6、cTnI浓度及CK-MB活性降低(P＜0.05);电镜结果显示:EI组心肌细胞损伤程度较IR组和L组减轻.结论 乳化异氟醚预处理可通过降低NF-κB活性抑制炎性反应,从而减轻大鼠心肌缺血再灌注损伤.
目的 探討乳化異氟醚預處理對大鼠心肌缺血再灌註時NF-κB活性的影響.方法 健康雄性SD大鼠48隻,體重230～280 g,隨機分為4組(n=12):假手術組(S組)僅穿線不結扎;缺血再灌註組(IR組)結扎左冠狀動脈前降支30 min,恢複灌註120 min製備心肌缺血再灌註損傷模型;脂肪乳劑組(L組)經股靜脈輸註30%脂肪乳劑4 ml·kg-1·h-1 30 min後製備模型;乳化異氟醚組(EI組)經股靜脈輸註乳化異氟醚4 ml·kg-1·h-130 min,洗脫15 min後製備模型.于再灌註120 min時採集股動脈血樣,採用ELISA法測定血清心肌肌鈣蛋白Ⅰ(cTnI)、IL-6濃度及燐痠肌痠激酶同工酶(CK-MB)活性,隨後處死大鼠取心髒,採用Western blot法測定心肌細胞NF-κB活性,電鏡下觀察心肌細胞的超微結構.結果 與S組比較,IR組、EI組和L組心肌細胞NF-κB活性升高,血清IL-6、cTnI濃度及CK-MB活性升高(P＜0.05或0.01);與IR組比較,ET組心肌細胞NF-κB活性降低,血清IL-6、cTnI濃度及CK-MB活性降低(P＜0.05);電鏡結果顯示:EI組心肌細胞損傷程度較IR組和L組減輕.結論 乳化異氟醚預處理可通過降低NF-κB活性抑製炎性反應,從而減輕大鼠心肌缺血再灌註損傷.
목적 탐토유화이불미예처리대대서심기결혈재관주시NF-κB활성적영향.방법 건강웅성SD대서48지,체중230～280 g,수궤분위4조(n=12):가수술조(S조)부천선불결찰;결혈재관주조(IR조)결찰좌관상동맥전강지30 min,회복관주120 min제비심기결혈재관주손상모형;지방유제조(L조)경고정맥수주30%지방유제4 ml·kg-1·h-1 30 min후제비모형;유화이불미조(EI조)경고정맥수주유화이불미4 ml·kg-1·h-130 min,세탈15 min후제비모형.우재관주120 min시채집고동맥혈양,채용ELISA법측정혈청심기기개단백Ⅰ(cTnI)、IL-6농도급린산기산격매동공매(CK-MB)활성,수후처사대서취심장,채용Western blot법측정심기세포NF-κB활성,전경하관찰심기세포적초미결구.결과 여S조비교,IR조、EI조화L조심기세포NF-κB활성승고,혈청IL-6、cTnI농도급CK-MB활성승고(P＜0.05혹0.01);여IR조비교,ET조심기세포NF-κB활성강저,혈청IL-6、cTnI농도급CK-MB활성강저(P＜0.05);전경결과현시:EI조심기세포손상정도교IR조화L조감경.결론 유화이불미예처리가통과강저NF-κB활성억제염성반응,종이감경대서심기결혈재관주손상.
Objective To investigate the effects of emulsified isoflurane preconditioning on myocardial NF-κB activity during ischemia-reperfusion(I/R)in rats.Methods Forty-eight healthy male SD rats weighing 230-280 g were randomly divided into 4 groups with 12 animals in each group: sham operation group(group S),I/R group,lipid emulsion + I/R group(group L)and emulsified isoflurane + I/R group(group EI).In group I/R,EI and L,myocardial I/R was produced by occlusion of left coronary anterior descending artery(LAD)for 30 min followed by 120 min reperfusion.In group L,30% lipid emulsion 4 ml·kg-1 ·h-1 was infused intravenously for 30 min before myocardial I/R.In group EI,emulsified isoflurane 4 ml· kg- 1 · h- 1 was infused intravenously for 30 min followed by 15 min washout before myocardial I/R.In group S and I/R,normal saline was given instead.Blood samples were taken from femoral artery at the end of 120 min reperfusion for determination of serum cTnI and IL-6 concentrations and CK-MB activity by ELISA.The rats were then killed and the myocardial tissues were taken for determination of NF-κB activity by Western blot and observation of the ultrastructure by electron microscopy.Results The NF-κB activity,serum cTnI and IL-6 concentrations and CK-MB activity were significantly higher in group I/R,EI and L than in group S(P ＜ 0.05 or 0.01),while lower in group EI than in group I/R(P ＜ 0.05).Microscopic examination showed that emulsified isoflurane significantly attenuated the histopathological changes in group EI.Conclusion Emulsified isoflurane pretreatment can attenuate myocardial I/R injury through decreasing the NF-κB activity and inhibiting inflammatory response in rats.
