CAJ | 학술논문

目的对中国贵州雷山苗族和三都水族群体胱硫醚-β-合酶基因C699T进行多态性分析,计算其基因频率,获取相应的遗传信息.方法采用nest-PCR和变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术寻找突变基因,对突变基因的PCR产物进行基因直接测序,并与文献报道的各种人群的相应数据进行比较.结果雷山苗族和三都水族群体的胱硫醚-β-合酶基因699T等位基因频率分别为5.1%和5.4%.结论雷山苗族和三都水族两个群体间胱硫醚-β-合酶基因699T等位基因差异无统计学意义,但它们均显著低于黑种人和白种人群体.
목적 대중국귀주뇌산묘족화삼도수족군체광류미-β-합매기인C699T진행다태성분석,계산기기인빈솔,획취상응적유전신식.방법 채용nest-PCR화변성제도응효전영(denaturing gradient gel electrophoresis,DGGE)기술심조돌변기인,대돌변기인적PCR산물진행기인직접측서,병여문헌보도적각충인군적상응수거진행비교.결과 뇌산묘족화삼도수족군체적광류미-β-합매기인699T등위기인빈솔분별위5.1%화5.4%.결론 뇌산묘족화삼도수족량개군체간광류미-β-합매기인699T등위기인차이무통계학의의,단타문균현저저우흑충인화백충인군체.
Objective To investigate the polymorphism of Cystathionine β-synthase in Miao ethnic and Shui ethnic population of Guizhou in China, and to obtain polymorphism information in Chinese minority.Methods The polymorphism C699T of Cystathionine β-synthase gene was amplified with nest-PCR and genotypes were determined with denaturing gradient gel electrophoresis (DGGE) and gene sequencing. Results The T allele frequencies at the site of CBS 699 of Miao minority in Leishan county and Shui population in Sandu county was 5.1% and 5.4%, respectively. Conclusion There is no statistical difference in terms of the genotype frequencies of C699T in two minority groups, but the results showed that significant difference was present between these two minorities group and White, Black ethics for the frequency of C699T mutation in the CBS gene.