眼科研究
眼科研究
안과연구
CHINESE OPHTHALMIC RESEARCH
2009年
12期
1095-1099
,共5页
侯正玉%卞小芸%宋秀君%左艳霞
侯正玉%卞小蕓%宋秀君%左豔霞
후정옥%변소예%송수군%좌염하
bcl-2%bax%0.2％酒石酸溴莫尼定%视神经夹挫伤
bcl-2%bax%0.2％酒石痠溴莫尼定%視神經夾挫傷
bcl-2%bax%0.2％주석산추막니정%시신경협좌상
bcl-2%bax%brimonidine tartrate%optic nerve crush injury
目的 观察0.2%酒石酸溴莫尼定对视神经夹挫伤大鼠视网膜形态及bcl-2/bax表达的影响,探讨其作用机制.方法 雌性SD大鼠90只随机分为正常组、模型组、治疗组,每组30只.60只大鼠用视神经钳夹法制作SD大鼠视神经夹挫伤模型,治疗组30只鼠给予0.2%酒石酸溴莫尼定点眼,于实验的l、3、5、7、14、21 d处死大鼠,用苏木精-伊红染色计数各组鼠视网膜神经节细胞(RGCs)的数量,用透射电镜观察和比较各组鼠视网膜的超微结构改变,免疫组织化学染色检测鼠视网膜中bcl-2及bax的表达.结果 正常组视网膜结构正常,治疗组较模型组视网膜形态损伤减轻.造模后3～21 d,模型组和治疗组较正常对照组RGCs数量明显减少(P<0.05),但治疗组较模型组RGCs数量明显增加(P<0.01).造模后5～7 d,治疗组bcl-2在鼠视网膜中的表达量较模型组明显增加(P<0.01),但bax表达量明显减少(P<0.01).模型组和治疗组的bcl-2在鼠视网膜中的表达量较正常组增加但bax表达量明显减少(P<0.05).结论 0.2%酒石酸溴莫尼定对大鼠视神经夹挫伤有一定的治疗作用,其机制与抑制凋亡有关.
目的 觀察0.2%酒石痠溴莫尼定對視神經夾挫傷大鼠視網膜形態及bcl-2/bax錶達的影響,探討其作用機製.方法 雌性SD大鼠90隻隨機分為正常組、模型組、治療組,每組30隻.60隻大鼠用視神經鉗夾法製作SD大鼠視神經夾挫傷模型,治療組30隻鼠給予0.2%酒石痠溴莫尼定點眼,于實驗的l、3、5、7、14、21 d處死大鼠,用囌木精-伊紅染色計數各組鼠視網膜神經節細胞(RGCs)的數量,用透射電鏡觀察和比較各組鼠視網膜的超微結構改變,免疫組織化學染色檢測鼠視網膜中bcl-2及bax的錶達.結果 正常組視網膜結構正常,治療組較模型組視網膜形態損傷減輕.造模後3～21 d,模型組和治療組較正常對照組RGCs數量明顯減少(P<0.05),但治療組較模型組RGCs數量明顯增加(P<0.01).造模後5～7 d,治療組bcl-2在鼠視網膜中的錶達量較模型組明顯增加(P<0.01),但bax錶達量明顯減少(P<0.01).模型組和治療組的bcl-2在鼠視網膜中的錶達量較正常組增加但bax錶達量明顯減少(P<0.05).結論 0.2%酒石痠溴莫尼定對大鼠視神經夾挫傷有一定的治療作用,其機製與抑製凋亡有關.
목적 관찰0.2%주석산추막니정대시신경협좌상대서시망막형태급bcl-2/bax표체적영향,탐토기작용궤제.방법 자성SD대서90지수궤분위정상조、모형조、치료조,매조30지.60지대서용시신경겸협법제작SD대서시신경협좌상모형,치료조30지서급여0.2%주석산추막니정점안,우실험적l、3、5、7、14、21 d처사대서,용소목정-이홍염색계수각조서시망막신경절세포(RGCs)적수량,용투사전경관찰화비교각조서시망막적초미결구개변,면역조직화학염색검측서시망막중bcl-2급bax적표체.결과 정상조시망막결구정상,치료조교모형조시망막형태손상감경.조모후3～21 d,모형조화치료조교정상대조조RGCs수량명현감소(P<0.05),단치료조교모형조RGCs수량명현증가(P<0.01).조모후5～7 d,치료조bcl-2재서시망막중적표체량교모형조명현증가(P<0.01),단bax표체량명현감소(P<0.01).모형조화치료조적bcl-2재서시망막중적표체량교정상조증가단bax표체량명현감소(P<0.05).결론 0.2%주석산추막니정대대서시신경협좌상유일정적치료작용,기궤제여억제조망유관.
Objective Present study aimed to research the mechanism of the use of 0.2% brimonidine tartrate eye drops in the treatment of optic nerve crush injury of rat.MethodsAnimal models of optic nerve crush injury were created in 60 SD female rats by clipping the exposed optic nerve at 2 mm in retrobulbar for 6 seconds with 78 grams of reverse forceps.The successful model was identified as Marcus-gun pupil without bleeding of fundus after operation.The animals were randomly assigned to model group and brimonidine treating group,and another 30 normal SD rats were used as the normal control group.The 0.2% brimonidine tartrate eye drops was topically administered at 2 hours before operation and after operation twice per day in the brimonidine treating group.The retinas from 18 rats were isolated after 3,7,21 days for RGCs counting by H&E staining,and the retinal ultrastructure was examined under the transmission electron microscope.The retinas from the other 72 SD rats (including normal,model and brimonidine groups) were prepared for the detection of bcl-2 and bax using immunohistochemistry l,3,5,7,14,21 days after the operation.ResultsNormal and almost normal retina structure was exhibited in rats of the normal group and brimonidine treating group,but disorder of cellular arragement and decrease of retinal thickness were found in the model rats under the optical microscope.The RGCs counting was significantly different among the three groups from 3 days through 21 days after operation with the considerably declination in the model group and brimonidine treating group compared with the normal control group (P＜0.05-0.01).However,that of the brimonidine treating group was obviously increased in comparison with the model group (P＜0.01).The expression of bax in rat retina was obviously reduced (P＜0.01),but the expression of bcl-2 was increased in brimonidine group compared with the model group from 5 through 7 days after operation (P＜0.01).ConclusionThe 0.2% brimonidine tartrate eye drops has a preventive effect on optic nerve crush injury of rat,and its inhibition on apoptosis is one of the mechanisms.
