中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2011年
6期
367-371
,共5页
模拟微重力%三维培养%甲状旁腺%塞尔托利细胞
模擬微重力%三維培養%甲狀徬腺%塞爾託利細胞
모의미중력%삼유배양%갑상방선%새이탁리세포
Weighlessness simulation%3-dimension culture%Parathyroid%Sertoli cells
目的 观察模拟微重力条件下三维培养的大鼠甲状旁腺细胞与同系睾丸塞尔托利细胞联合移植后的分泌功能,以及移植物存活情况.方法 取SD大鼠的甲状旁腺细胞,分别进行常规培养和模拟微重力条件下三维培养;取SD大鼠睾丸塞尔托利细胞,进行常规培养.取Wistar大鼠作为移植受者,制备甲状旁腺功能低下症模型.用随机数字表法将建模成功的Wistar大鼠分为3组:A组大鼠接受常规培养的单纯甲状旁腺细胞移植;B组大鼠接受常规培养的甲状旁腺细胞联合睾丸塞尔托利细胞移植;C组大鼠接受经三维培养的甲状旁腺细胞联合睾丸塞尔托利细胞移植.移植后观察各组移植物的存活情况,并检测移植物的细胞成分,淋巴细胞凋亡及甲状旁腺细胞功能.结果 A组、B组和C组移植物的存活时间依次延长,分别为(17.3±1.6)d、(43.2±2.4)d和(52.5±1.5)d,3组间两两比较,差异均有统计学意义(P＜0.05).C组移植物内甲状旁腺细胞已长入支架材料内部,并与之紧密结合,甲状旁腺激素分泌旺盛;还可见大量散在的、Fas配体表达阳性的睾丸塞尔托利细胞;在移植物与肾实质交界处可见大量淋巴细胞凋亡.结论 模拟微重力条件下三维培养技术与具有免疫赦免作用的睾丸sertoli细胞共同应用于甲状旁腺细胞移植,可以提高移植后甲状旁腺细胞的分泌功能,延长移植物的存活时间.
目的 觀察模擬微重力條件下三維培養的大鼠甲狀徬腺細胞與同繫睪汍塞爾託利細胞聯閤移植後的分泌功能,以及移植物存活情況.方法 取SD大鼠的甲狀徬腺細胞,分彆進行常規培養和模擬微重力條件下三維培養;取SD大鼠睪汍塞爾託利細胞,進行常規培養.取Wistar大鼠作為移植受者,製備甲狀徬腺功能低下癥模型.用隨機數字錶法將建模成功的Wistar大鼠分為3組:A組大鼠接受常規培養的單純甲狀徬腺細胞移植;B組大鼠接受常規培養的甲狀徬腺細胞聯閤睪汍塞爾託利細胞移植;C組大鼠接受經三維培養的甲狀徬腺細胞聯閤睪汍塞爾託利細胞移植.移植後觀察各組移植物的存活情況,併檢測移植物的細胞成分,淋巴細胞凋亡及甲狀徬腺細胞功能.結果 A組、B組和C組移植物的存活時間依次延長,分彆為(17.3±1.6)d、(43.2±2.4)d和(52.5±1.5)d,3組間兩兩比較,差異均有統計學意義(P＜0.05).C組移植物內甲狀徬腺細胞已長入支架材料內部,併與之緊密結閤,甲狀徬腺激素分泌旺盛;還可見大量散在的、Fas配體錶達暘性的睪汍塞爾託利細胞;在移植物與腎實質交界處可見大量淋巴細胞凋亡.結論 模擬微重力條件下三維培養技術與具有免疫赦免作用的睪汍sertoli細胞共同應用于甲狀徬腺細胞移植,可以提高移植後甲狀徬腺細胞的分泌功能,延長移植物的存活時間.
목적 관찰모의미중력조건하삼유배양적대서갑상방선세포여동계고환새이탁리세포연합이식후적분비공능,이급이식물존활정황.방법 취SD대서적갑상방선세포,분별진행상규배양화모의미중력조건하삼유배양;취SD대서고환새이탁리세포,진행상규배양.취Wistar대서작위이식수자,제비갑상방선공능저하증모형.용수궤수자표법장건모성공적Wistar대서분위3조:A조대서접수상규배양적단순갑상방선세포이식;B조대서접수상규배양적갑상방선세포연합고환새이탁리세포이식;C조대서접수경삼유배양적갑상방선세포연합고환새이탁리세포이식.이식후관찰각조이식물적존활정황,병검측이식물적세포성분,림파세포조망급갑상방선세포공능.결과 A조、B조화C조이식물적존활시간의차연장,분별위(17.3±1.6)d、(43.2±2.4)d화(52.5±1.5)d,3조간량량비교,차이균유통계학의의(P＜0.05).C조이식물내갑상방선세포이장입지가재료내부,병여지긴밀결합,갑상방선격소분비왕성;환가견대량산재적、Fas배체표체양성적고환새이탁리세포;재이식물여신실질교계처가견대량림파세포조망.결론 모의미중력조건하삼유배양기술여구유면역사면작용적고환sertoli세포공동응용우갑상방선세포이식,가이제고이식후갑상방선세포적분비공능,연장이식물적존활시간.
Objective To observe the function and survival of parathyroid cells cultured under simulated microgravity condition after cotransplanation of syngeneic allogeneic testicular sertoli cells.Methods Parathyroid cells in SD rats were assigned to flask-culture or bioreactor-culture.Allogeneic testicular sertoli cells in SD rats were cultured by using routine method.The recipients of hypoparathyroidism Wistar rat models were divided into 3 groups randomly:group A,receiving parathyroid cells(cultured with routine method)transplantation only;group B,receiving parathyroid cells and allogeneic testicular sertoli cells(cultured with routine method)transplantation;group C,receiving parathyroid cells(cultured under simulated microgravity condition)and allogeneic testicular sertoli cells transplantation.Allograft survival,change in cell components,apoptosis of infiltrative lymphocytes and parathyroid cells function were analyzed after transplantation respectively.Results The average survival time in group A,B and C was(17.3±1.6),(43.2±2.4)and (52.5±1.5)days,respectively.There was significant difference among group (P<0.05).In group C,parathyroid cells with strong secreting function grew into scaffold materials and adhered to them.FasL-expressing testicular cells and apoptotic lymphocytes were quite evident between allograft and kidney parenchyma.Conclusion Parathyroid cell cultured under simulated microgravity condition enhances its survival and function after cotransplanation of allogeneic testicular sertoli cell with immune privilege.
