CAJ | 학술논문

背景:乙二醛的毒性较低,主要表现为对眼及呼吸道粘膜的刺激作用.近年研究表明,它还有致癌性和致突变性,并与脂质过氧化作用关系密切,可诱发活性氧自由基产生. 目的:通过观察乙二醛染毒小鼠全血及组织中谷胱甘肽过氧化物酶 (GSH-Px)及超氧化物歧化酶 (SOD)活力的变化,探讨乙二醛对抗氧化酶活力的影响. 设计:完全随机设计,安慰剂对照. 地点和对象:参与者均来自沈阳市卫生系统.选用健康成年昆明种小鼠 32只,雌雄各半,由中国医科大学实验动物部提供. 干预:分为实验组(高、中、低剂量组)和对照组分别给小鼠腹腔注射不同浓度的乙二醛,对照组注射生理盐水, 24 h后经摘眼球法采集血样,将小鼠颈椎脱臼处死后取心、肝、肾、和脑组织,并制成匀浆. 主要观察指标:分别采用二硫双硝基苯甲酸( DTNB)法及亚硝酸盐法测定全血及组织中 GSH-Px及 SOD活力. 结果:全血和肾脏的低、中、高剂量组 GSH Px活力显著低于对照组,心、脑、肾的高剂量组 SOD活力(μ kat/g)分别为 2132± 404, 878± 302, 1730± 123,对照组 SOD活力(μ kat/g)分别为 1519± 346, 418± 76, 1463± 172,两组比较,差异有极显著性意义( q值分别为 9.81, 13.06, 4.97, P< 0.01),肝脏的低、中剂量组 SOD活力与对照组相比,差异具有显著性意义( P< 0.05). 结论:乙二醛对全血及肾脏中 GSH-Px活力的影响主要表现为抑制作用,对肝脏和脑中 GSH-Px活力的影响主要为应激性增高.乙二醛对组织中 SOD活力的影响主要表现为应激性增高趋势.
배경:을이철적독성교저,주요표현위대안급호흡도점막적자격작용.근년연구표명,타환유치암성화치돌변성,병여지질과양화작용관계밀절,가유발활성양자유기산생. 목적:통과관찰을이철염독소서전혈급조직중곡광감태과양화물매 (GSH-Px)급초양화물기화매 (SOD)활력적변화,탐토을이철대항양화매활력적영향. 설계:완전수궤설계,안위제대조. 지점화대상:삼여자균래자침양시위생계통.선용건강성년곤명충소서 32지,자웅각반,유중국의과대학실험동물부제공. 간예:분위실험조(고、중、저제량조)화대조조분별급소서복강주사불동농도적을이철,대조조주사생리염수, 24 h후경적안구법채집혈양,장소서경추탈구처사후취심、간、신、화뇌조직,병제성균장. 주요관찰지표:분별채용이류쌍초기분갑산( DTNB)법급아초산염법측정전혈급조직중 GSH-Px급 SOD활력. 결과:전혈화신장적저、중、고제량조 GSH Px활력현저저우대조조,심、뇌、신적고제량조 SOD활력(μ kat/g)분별위 2132± 404, 878± 302, 1730± 123,대조조 SOD활력(μ kat/g)분별위 1519± 346, 418± 76, 1463± 172,량조비교,차이유겁현저성의의( q치분별위 9.81, 13.06, 4.97, P< 0.01),간장적저、중제량조 SOD활력여대조조상비,차이구유현저성의의( P< 0.05). 결론:을이철대전혈급신장중 GSH-Px활력적영향주요표현위억제작용,대간장화뇌중 GSH-Px활력적영향주요위응격성증고.을이철대조직중 SOD활력적영향주요표현위응격성증고추세.
BACKGROUND:In the past,it has been thought that the toxicity of glyoxal is low and mainly associated with stimulation in the eyes and the mucosa of the respiratory tract.It has been suggested that glyoxal is a carcinogen and mutagen in recent studies.In addition,it has a close relationship with lipid peroxidation,and it may increase the formation of active oxygenic products.OBJECTIVE:To study effects of glyoxal on anti oxidase in mice by observing the activity changes of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) in whole blood and tissues after exposure to glyoxal.DESIGN:Mice were divided into treatment groups and negative control group by completely random design.The placebo control was used in this study.SETTING AND PARTICIPANTS:Participants were recruited from healthy system in Shenyang.A total of 32 Kunming mice,with equal ratio of male and female,were provided by the Department of Laboratory Animal,China Medical University.INTERVENTION:The mice were given glyoxal by intraperitoneal injection and they were anesthetized and blood was taken by excising the eyeballs after they were administrated for 24 hours.The animals were killed and their hearts,liver,brain and kidney were taken out.The tissues were prepared for seriflux before being determined.MAIN OUTCOME MEASURE:Activities of GSH-Px and SOD in whole blood and tissues were determined by dithiobinitrobenzoic acid(DTNB) and nitrite assay repectively.RESULTS:In whole blood and kidney,GSH-Px activity of low, mid and high dose groups was significantly lower than that of the control.SOD activities(μ kat/g) of heart,brain and kidney protein in high dose group were 2132± 404,878± 302,1730± 123 respectively,and in the control groups were 1519± 346,418± 76,1463± 172 respectively.There was a significant difference between the dose groups and the control groups(q=9.81,13.06,4.97,P< 0.01).SOD activities of liver protein in the low and mid dose group were significantly different from that of the control group(P< 0.05).CONCLUSION:Glyoxal exposure inhibits the GSH-Px activity in whole blood and kidney,while increases in liver, and also increases SOD activity in tissues.