CAJ | 학술논문

目的研究骨髓间充质干细胞(MSCs)对小鼠Lewis肺癌皮下移植瘤的影响.方法自C57BL/6小鼠骨髓分离MSCs,制备单细胞悬液并于体外传代培养,取第4～5代细胞用于实验.56只C57BL/6小鼠经Lewis肺癌细胞皮下接种建立小鼠肺癌皮下移植瘤模型,根据MSCs给予时间分为D0组(接种同时给予MSCs)和D10组(接种后第10天给予MSCs),其中D0组分为3个亚组(n=8):组1单纯接种肿瘤细胞,组2肿瘤细胞和MSCs共同接种,组3肿瘤细胞接种及尾静脉注射MSCs;D10组分为4个亚组(n=8):组4(肿瘤细胞接种及瘤体内注射MSCs)及其等量PBS对照组(组5),组6(肿瘤细胞接种及尾静脉注射MSCs)及其等量PBS对照组(组7).观察各组移植肿瘤的生长情况,包括肿瘤形成时间及不同时间点的瘤体大小,并进行组间分析比较.结果与组1和组3比较,组2的肿瘤形成时间明显缩短(P<0.05);而各时间点三组瘤体大小比较,差异无统计学意义(P>0.05).组4的瘤体显著大于其对照组(P<0.05);而组6与其对照组的瘤体大小比较,差异无统计学意义(P>0.05).结论 MSCs与Lewis细胞同时接种可加速小鼠肺癌皮下移植瘤形成,而成瘤后MSCs瘤体内注射具有促进移植瘤生长的作用.
목적 연구골수간충질간세포(MSCs)대소서Lewis폐암피하이식류적영향.방법 자C57BL/6소서골수분리MSCs,제비단세포현액병우체외전대배양,취제4～5대세포용우실험.56지C57BL/6소서경Lewis폐암세포피하접충건립소서폐암피하이식류모형,근거MSCs급여시간분위D0조(접충동시급여MSCs)화D10조(접충후제10천급여MSCs),기중D0조분위3개아조(n=8):조1단순접충종류세포,조2종류세포화MSCs공동접충,조3종류세포접충급미정맥주사MSCs;D10조분위4개아조(n=8):조4(종류세포접충급류체내주사MSCs)급기등량PBS대조조(조5),조6(종류세포접충급미정맥주사MSCs)급기등량PBS대조조(조7).관찰각조이식종류적생장정황,포괄종류형성시간급불동시간점적류체대소,병진행조간분석비교.결과 여조1화조3비교,조2적종류형성시간명현축단(P<0.05);이각시간점삼조류체대소비교,차이무통계학의의(P>0.05).조4적류체현저대우기대조조(P<0.05);이조6여기대조조적류체대소비교,차이무통계학의의(P>0.05).결론 MSCs여Lewis세포동시접충가가속소서폐암피하이식류형성,이성류후MSCs류체내주사구유촉진이식류생장적작용.
Objective To investigate the effect of mesenchymal stem cells (MSCs) on subcutaneous xenograft tumors in mice with Lewis lung cancer. Methods MSCs isolated from bone marrow of C57BL/6 mice were made into single cell suspension and were cultured in vitro. The cells of the 4th to 5th passage were used for the subsequent experiments. Fifty six C57BL/6 mice were inoculated subcutaneously with Lewis lung cancer cells, and were grouped into Group D0 (MSCs were given simultaneously with inoculation)and Group D10(MSCs were given 10 d after inoculation). Group D0 included three subgroups (n=8): Group 1 with inoculation of tumor cells, Group 2 with inoculation of tumor cells and MSCs, and Group 3 with inoculation of tumor cells and tail intravenous injection of MSCs. Group D10 included four groups: Group 4 with inoculation of tumor cells and injection of MSCs in tumors, Group 5 with equivalent PBS (the control of Group 4), Group 6 with inoculation of tumor cells and tail intravenous injection of MSCs, and Group 7 with equivalent PBS (the control of Group 6). The time of tumor formation and the volume of tumor were observed and compared among the groups. ResultsIn Group D0, earlier onset of tumor development was observed in Group 2 as compared to Group 1 and Group 3 (P<0.05), while there was no significant difference on the volume of tumor in the three groups (P>0.05). In Group D10, the volume of tumors were larger in Group 4 compared to the control (P<0.05), while there was no significant difference on the volume of tumors between Group 6 and the control (P>0.05). Conclusion Inoculating mixture of MSCs and Lewis lung cancer cells accelerates tumor formation,and injection of MSCs in tumors stimulates the growth of tumors.